Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | LOXIMVI | 2.0000 | uM | 24 | hr | | Ki-67 | 2059.50 | p-Rb(S807/S811) | 90.66 | p-cJun(S73) | 86.47 | 1 | LOXIMVI-signal3 | B3 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 165.37 | p-Rb(S807/S811) | 90.82 | p-S6(S235/S236 | 359.54 | 1 | WM1552C-signal2 | F6 |
| Vemurafenib | WM1552C | 0.0512 | uM | 24 | hr | | Ki-67 | 1417.80 | p-Rb(S807/S811) | 90.84 | p-cJun(S73) | 153.01 | 2 | WM1552C-signal3 | C7 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 231.93 | p-Rb(S807/S811) | 91.17 | p-S6(S235/S236 | 89.76 | 2 | LOXIMVI-signal2 | G3 |
| Vemurafenib | LOXIMVI | 2.0000 | uM | 24 | hr | | p27 Kip1 | 457.12 | p-Rb(S807/S811) | 91.96 | p-ERK(T202/Y204) | 30.10 | 2 | LOXIMVI-signal1 | C3 | |
| Vemurafenib | LOXIMVI | 2.0000 | uM | 24 | hr | | Ki-67 | 2153.30 | p-Rb(S807/S811) | 92.72 | p-cJun(S73) | 84.91 | 2 | LOXIMVI-signal3 | C3 | |
| Vemurafenib | WM1552C | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 230.57 | p-Rb(S807/S811) | 92.72 | p-S6(S235/S236 | 359.12 | 2 | WM1552C-signal2 | C6 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.8000 | uM | 24 | hr | | Ki-67 | 2249.30 | p-Rb(S807/S811) | 93.65 | p-cJun(S73) | 24.47 | 1 | LOXIMVI-signal3 | F4 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 2.0000 | uM | 24 | hr | | Ki-67 | 2379.60 | p-Rb(S807/S811) | 93.92 | p-cJun(S73) | 26.61 | 1 | LOXIMVI-signal3 | F3 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0512 | uM | 24 | hr | | Ki-67 | 1383.60 | p-Rb(S807/S811) | 94.09 | p-cJun(S73) | 155.54 | 2 | WM1552C-signal3 | G7 |
| Vemurafenib | WM1552C | 0.0512 | uM | 24 | hr | | p27 Kip1 | 262.18 | p-Rb(S807/S811) | 94.16 | p-ERK(T202/Y204) | 164.16 | 2 | WM1552C-signal1 | C7 | |
| Vemurafenib | LOXIMVI | 2.0000 | uM | 24 | hr | | p27 Kip1 | 456.75 | p-Rb(S807/S811) | 94.75 | p-ERK(T202/Y204) | 28.87 | 1 | LOXIMVI-signal1 | B3 | |
| Vemurafenib | WM1552C | 0.0512 | uM | 24 | hr | | p27 Kip1 | 269.92 | p-Rb(S807/S811) | 94.99 | p-ERK(T202/Y204) | 160.06 | 1 | WM1552C-signal1 | B7 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0512 | uM | 24 | hr | | Ki-67 | 1457.20 | p-Rb(S807/S811) | 95.63 | p-cJun(S73) | 146.98 | 1 | WM1552C-signal3 | F7 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 232.69 | p-Rb(S807/S811) | 95.77 | p-S6(S235/S236 | 414.55 | 2 | WM1552C-signal2 | G6 |
| Vemurafenib | WM1552C | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 224.64 | p-Rb(S807/S811) | 95.99 | p-S6(S235/S236 | 373.81 | 2 | WM1552C-signal2 | C7 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 273.71 | p-Rb(S807/S811) | 96.01 | p-ERK(T202/Y204) | 160.08 | 2 | WM1552C-signal1 | G7 |
| Vemurafenib | WM1552C | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 208.13 | p-Rb(S807/S811) | 96.36 | p-S6(S235/S236 | 376.62 | 1 | WM1552C-signal2 | B6 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | Ki-67 | 2211.10 | p-Rb(S807/S811) | 96.70 | p-cJun(S73) | 24.08 | 2 | Colo858-signal3 | G2 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 190.31 | p-Rb(S807/S811) | 96.89 | p-S6(S235/S236 | 375.87 | 1 | WM1552C-signal2 | F7 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 5.0000 | uM | 24 | hr | | Ki-67 | 2814.00 | p-Rb(S807/S811) | 96.96 | p-cJun(S73) | 30.63 | 1 | LOXIMVI-signal3 | F2 |
| Vemurafenib | LOXIMVI | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 203.52 | p-Rb(S807/S811) | 97.55 | p-S6(S235/S236 | 181.29 | 1 | LOXIMVI-signal2 | B3 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 191.70 | p-Rb(S807/S811) | 97.70 | p-S6(S235/S236 | 403.09 | 2 | WM1552C-signal2 | G7 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 229.67 | p-Rb(S807/S811) | 98.04 | p-S6(S235/S236 | 97.15 | 1 | LOXIMVI-signal2 | F3 |
| Vemurafenib | WM1552C | 0.0512 | uM | 24 | hr | | Ki-67 | 1451.30 | p-Rb(S807/S811) | 98.22 | p-cJun(S73) | 157.24 | 1 | WM1552C-signal3 | B7 |