Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | WM-115 | 0.0033 | uM | 24 | hr | | Ki-67 | 783.34 | p-Rb(S807/S811) | 75.14 | p-cJun(S73) | 46.08 | 1 | WM115-signal3 | D10 | |
| JNK-IN-8 | WM-115 | 0.0000 | uM | 24 | hr | | Ki-67 | 735.15 | p-Rb(S807/S811) | 75.29 | p-cJun(S73) | 50.13 | 2 | WM115-signal3 | E11 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0000 | uM | 24 | hr | | Ki-67 | 696.53 | p-Rb(S807/S811) | 75.37 | p-cJun(S73) | 50.95 | 2 | WM115-signal3 | G11 |
| JNK-IN-8 | WM-115 | 0.1280 | uM | 24 | hr | | Ki-67 | 748.11 | p-Rb(S807/S811) | 75.71 | p-cJun(S73) | 27.81 | 2 | WM115-signal3 | E6 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0000 | uM | 24 | hr | | Ki-67 | 782.51 | p-Rb(S807/S811) | 75.83 | p-cJun(S73) | 51.58 | 1 | WM115-signal3 | F11 |
| JNK-IN-8 | WM-115 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 222.05 | p-Rb(S807/S811) | 76.04 | p-ERK(T202/Y204) | 636.67 | 2 | WM115-signal1 | E10 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0082 | uM | 24 | hr | | Ki-67 | 782.06 | p-Rb(S807/S811) | 76.06 | p-cJun(S73) | 46.14 | 1 | WM115-signal3 | F9 |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | Ki-67 | 863.33 | p-Rb(S807/S811) | 76.07 | p-cJun(S73) | 41.68 | 1 | WM115-signal3 | F7 |
| JNK-IN-8 | WM-115 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 260.22 | p-Rb(S807/S811) | 76.23 | p-S6(S235/S236 | 189.51 | 2 | WM115-signal2 | E5 | |
| JNK-IN-8 | WM-115 | 5.0000 | uM | 24 | hr | | Ki-67 | 808.69 | p-Rb(S807/S811) | 76.27 | p-cJun(S73) | 22.54 | 2 | WM115-signal3 | E2 | |
| JNK-IN-8 | WM-115 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 236.12 | p-Rb(S807/S811) | 76.30 | p-S6(S235/S236 | 187.61 | 2 | WM115-signal2 | E7 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 158.64 | p-Rb(S807/S811) | 76.31 | p-S6(S235/S236 | 405.08 | 2 | WM1552C-signal2 | G5 |
| JNK-IN-8 | WM-115 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 205.29 | p-Rb(S807/S811) | 76.65 | p-S6(S235/S236 | 177.08 | 1 | WM115-signal2 | D5 | |
| JNK-IN-8 | WM-115 | 0.0205 | uM | 24 | hr | | Ki-67 | 798.51 | p-Rb(S807/S811) | 76.68 | p-cJun(S73) | 41.54 | 2 | WM115-signal3 | E8 | |
| JNK-IN-8 | WM-115 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 329.54 | p-Rb(S807/S811) | 76.68 | p-S6(S235/S236 | 190.63 | 2 | WM115-signal2 | E2 | |
| JNK-IN-8 | WM-115 | 0.0205 | uM | 24 | hr | | Ki-67 | 755.77 | p-Rb(S807/S811) | 76.94 | p-cJun(S73) | 41.65 | 1 | WM115-signal3 | D8 | |
| Vemurafenib | COLO 858 | 0.1280 | uM | 24 | hr | | Ki-67 | 972.60 | p-Rb(S807/S811) | 76.98 | p-cJun(S73) | 64.88 | 1 | Colo858-signal3 | B6 | |
| JNK-IN-8 | WM-115 | 0.0512 | uM | 24 | hr | | Ki-67 | 781.07 | p-Rb(S807/S811) | 77.07 | p-cJun(S73) | 34.92 | 1 | WM115-signal3 | D7 | |
| JNK-IN-8 | WM-115 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 230.19 | p-Rb(S807/S811) | 77.30 | p-ERK(T202/Y204) | 802.24 | 1 | WM115-signal1 | D2 | |
| JNK-IN-8 | WM-115 | 2.0000 | uM | 24 | hr | | Ki-67 | 790.07 | p-Rb(S807/S811) | 77.44 | p-cJun(S73) | 19.89 | 1 | WM115-signal3 | D3 | |
| JNK-IN-8 | WM-115 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 243.03 | p-Rb(S807/S811) | 77.65 | p-S6(S235/S236 | 178.01 | 2 | WM115-signal2 | E6 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 227.51 | p-Rb(S807/S811) | 77.75 | p-ERK(T202/Y204) | 515.73 | 1 | WM115-signal1 | F8 |
| JNK-IN-8 | WM-115 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 226.04 | p-Rb(S807/S811) | 77.90 | p-ERK(T202/Y204) | 670.95 | 2 | WM115-signal1 | E9 | |
| JNK-IN-8 | WM-115 | 0.1280 | uM | 24 | hr | | Ki-67 | 843.01 | p-Rb(S807/S811) | 77.92 | p-cJun(S73) | 26.33 | 1 | WM115-signal3 | D6 | |
| JNK-IN-8 | WM-115 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 248.53 | p-Rb(S807/S811) | 77.96 | p-S6(S235/S236 | 209.55 | 1 | WM115-signal2 | D2 |