Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | COLO 858 | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 271.62 | p-Rb(S807/S811) | 67.91 | p-S6(S235/S236 | 39.37 | 2 | Colo858-signal2 | G3 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 273.71 | p-Rb(S807/S811) | 96.01 | p-ERK(T202/Y204) | 160.08 | 2 | WM1552C-signal1 | G7 |
| Vemurafenib | WM-115 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 274.41 | p-Rb(S807/S811) | 61.27 | p-S6(S235/S236 | 22.13 | 1 | WM115-signal2 | F2 |
| Vemurafenib | COLO 858 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 274.60 | p-Rb(S807/S811) | 140.30 | p-S6(S235/S236 | 340.20 | 2 | Colo858-signal2 | C7 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 277.07 | p-Rb(S807/S811) | 41.08 | p-ERK(T202/Y204) | 52.64 | 1 | WM115-signal1 | F5 |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 277.38 | p-Rb(S807/S811) | 42.72 | p-ERK(T202/Y204) | 54.07 | 2 | WM115-signal1 | G5 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 277.62 | p-Rb(S807/S811) | 113.74 | p-S6(S235/S236 | 415.75 | 2 | WM1552C-signal2 | G11 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 279.48 | p-Rb(S807/S811) | 87.64 | p-ERK(T202/Y204) | 117.21 | 1 | WM1552C-signal1 | F6 |
| Vemurafenib | LOXIMVI | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 280.25 | p-Rb(S807/S811) | 186.93 | p-S6(S235/S236 | 402.53 | 2 | LOXIMVI-signal2 | C6 | |
| JNK-IN-8 | COLO 858 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 280.73 | p-Rb(S807/S811) | 169.28 | p-S6(S235/S236 | 409.65 | 1 | Colo858-signal2 | D10 | |
| JNK-IN-8 | LOXIMVI | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 281.63 | p-Rb(S807/S811) | 231.63 | p-S6(S235/S236 | 455.97 | 2 | LOXIMVI-signal2 | E8 | |
| Vemurafenib | LOXIMVI | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 282.64 | p-Rb(S807/S811) | 182.20 | p-S6(S235/S236 | 400.81 | 1 | LOXIMVI-signal2 | B6 | |
| JNK-IN-8 | LOXIMVI | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 282.86 | p-Rb(S807/S811) | 236.08 | p-S6(S235/S236 | 480.10 | 2 | LOXIMVI-signal2 | E6 | |
| JNK-IN-8 | LOXIMVI | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 283.24 | p-Rb(S807/S811) | 237.66 | p-S6(S235/S236 | 456.10 | 1 | LOXIMVI-signal2 | D10 | |
| JNK-IN-8 | LOXIMVI | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 283.60 | p-Rb(S807/S811) | 227.06 | p-S6(S235/S236 | 453.19 | 2 | LOXIMVI-signal2 | E7 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 284.31 | p-Rb(S807/S811) | 88.80 | p-ERK(T202/Y204) | 123.78 | 2 | WM1552C-signal1 | G6 |
| Vemurafenib | COLO 858 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 284.31 | p-Rb(S807/S811) | 139.79 | p-S6(S235/S236 | 349.95 | 1 | Colo858-signal2 | B7 | |
| Vemurafenib | WM1552C | 0.1280 | uM | 24 | hr | | p27 Kip1 | 284.96 | p-Rb(S807/S811) | 88.69 | p-ERK(T202/Y204) | 119.29 | 1 | WM1552C-signal1 | B6 | |
| JNK-IN-8 | LOXIMVI | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 285.05 | p-Rb(S807/S811) | 232.52 | p-S6(S235/S236 | 459.23 | 1 | LOXIMVI-signal2 | D7 | |
| JNK-IN-8 | WM1552C | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 285.27 | p-Rb(S807/S811) | 112.32 | p-S6(S235/S236 | 447.61 | 2 | WM1552C-signal2 | E2 | |
| JNK-IN-8 | LOXIMVI | 0.0205 | uM | 24 | hr | | p27 Kip1 | 286.32 | p-Rb(S807/S811) | 204.47 | p-ERK(T202/Y204) | 198.65 | 2 | LOXIMVI-signal1 | E8 | |
| JNK-IN-8 | COLO 858 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 288.25 | p-Rb(S807/S811) | 170.31 | p-S6(S235/S236 | 401.43 | 1 | Colo858-signal2 | D8 | |
| Vemurafenib | LOXIMVI | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 288.44 | p-Rb(S807/S811) | 221.82 | p-S6(S235/S236 | 462.92 | 1 | LOXIMVI-signal2 | B9 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 288.89 | p-Rb(S807/S811) | 152.75 | p-S6(S235/S236 | 379.48 | 1 | Colo858-signal2 | F10 |
| JNK-IN-8 | LOXIMVI | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 290.00 | p-Rb(S807/S811) | 244.26 | p-S6(S235/S236 | 469.02 | 1 | LOXIMVI-signal2 | D6 |