Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | WM1552C | 2.0000 | uM | 24 | hr | | p27 Kip1 | 257.75 | p-Rb(S807/S811) | 110.40 | p-ERK(T202/Y204) | 269.60 | 2 | WM1552C-signal1 | E3 | |
| JNK-IN-8 | WM-115 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 257.79 | p-Rb(S807/S811) | 79.63 | p-S6(S235/S236 | 207.22 | 2 | WM115-signal2 | E3 | |
| JNK-IN-8 | WM1552C | 2.0000 | uM | 24 | hr | | p27 Kip1 | 258.20 | p-Rb(S807/S811) | 113.91 | p-ERK(T202/Y204) | 277.27 | 1 | WM1552C-signal1 | D3 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 258.76 | p-Rb(S807/S811) | 210.04 | p-S6(S235/S236 | 429.29 | 2 | LOXIMVI-signal2 | G8 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 258.89 | p-Rb(S807/S811) | 123.29 | p-S6(S235/S236 | 272.97 | 2 | LOXIMVI-signal2 | G4 |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 259.21 | p-Rb(S807/S811) | 68.62 | p-S6(S235/S236 | 16.39 | 2 | Colo858-signal2 | C2 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 259.78 | p-Rb(S807/S811) | 60.44 | p-S6(S235/S236 | 25.02 | 2 | WM115-signal2 | G2 |
| JNK-IN-8 | WM-115 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 260.22 | p-Rb(S807/S811) | 76.23 | p-S6(S235/S236 | 189.51 | 2 | WM115-signal2 | E5 | |
| JNK-IN-8 | LOXIMVI | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 260.69 | p-Rb(S807/S811) | 241.93 | p-S6(S235/S236 | 501.67 | 2 | LOXIMVI-signal2 | E5 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 260.82 | p-Rb(S807/S811) | 220.20 | p-S6(S235/S236 | 459.82 | 2 | LOXIMVI-signal2 | G9 |
| Vemurafenib | WM-115 | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 261.27 | p-Rb(S807/S811) | 47.45 | p-S6(S235/S236 | 41.16 | 1 | WM115-signal2 | F3 |
| Vemurafenib | WM1552C | 0.0512 | uM | 24 | hr | | p27 Kip1 | 262.18 | p-Rb(S807/S811) | 94.16 | p-ERK(T202/Y204) | 164.16 | 2 | WM1552C-signal1 | C7 | |
| JNK-IN-8 | WM1552C | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 262.28 | p-Rb(S807/S811) | 113.37 | p-S6(S235/S236 | 413.92 | 1 | WM1552C-signal2 | D4 | |
| Vemurafenib | WM-115 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 263.18 | p-Rb(S807/S811) | 37.97 | p-ERK(T202/Y204) | 43.14 | 1 | WM115-signal1 | B5 | |
| Vemurafenib | LOXIMVI | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 264.06 | p-Rb(S807/S811) | 220.85 | p-S6(S235/S236 | 434.18 | 2 | LOXIMVI-signal2 | C9 | |
| Vemurafenib | LOXIMVI | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 265.14 | p-Rb(S807/S811) | 204.08 | p-S6(S235/S236 | 419.00 | 2 | LOXIMVI-signal2 | C8 | |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 265.46 | p-Rb(S807/S811) | 66.87 | p-S6(S235/S236 | 14.48 | 1 | Colo858-signal2 | B2 | |
| JNK-IN-8 | WM-115 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 267.33 | p-Rb(S807/S811) | 79.84 | p-ERK(T202/Y204) | 786.46 | 2 | WM115-signal1 | E2 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 267.75 | p-Rb(S807/S811) | 208.98 | p-S6(S235/S236 | 429.59 | 1 | LOXIMVI-signal2 | F8 |
| JNK-IN-8 | LOXIMVI | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 267.83 | p-Rb(S807/S811) | 225.75 | p-S6(S235/S236 | 426.66 | 1 | LOXIMVI-signal2 | D9 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 268.49 | p-Rb(S807/S811) | 89.43 | p-ERK(T202/Y204) | 154.75 | 1 | WM1552C-signal1 | F7 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 268.49 | p-Rb(S807/S811) | 170.62 | p-S6(S235/S236 | 412.34 | 2 | LOXIMVI-signal2 | G6 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 269.36 | p-Rb(S807/S811) | 224.35 | p-S6(S235/S236 | 447.46 | 1 | LOXIMVI-signal2 | F10 |
| Vemurafenib | WM1552C | 0.0512 | uM | 24 | hr | | p27 Kip1 | 269.92 | p-Rb(S807/S811) | 94.99 | p-ERK(T202/Y204) | 160.06 | 1 | WM1552C-signal1 | B7 | |
| Vemurafenib | WM1552C | 0.1280 | uM | 24 | hr | | p27 Kip1 | 271.43 | p-Rb(S807/S811) | 88.61 | p-ERK(T202/Y204) | 136.09 | 2 | WM1552C-signal1 | C6 |