Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 245.42 | p-Rb(S807/S811) | 98.27 | p-ERK(T202/Y204) | 209.11 | 2 | WM1552C-signal1 | G8 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 268.49 | p-Rb(S807/S811) | 89.43 | p-ERK(T202/Y204) | 154.75 | 1 | WM1552C-signal1 | F7 |
| JNK-IN-8 | WM1552C | 0.1280 | uM | 24 | hr | | p27 Kip1 | 230.98 | p-Rb(S807/S811) | 107.78 | p-ERK(T202/Y204) | 265.42 | 2 | WM1552C-signal1 | E6 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 478.70 | p-Rb(S807/S811) | 48.18 | p-ERK(T202/Y204) | 22.45 | 1 | WM1552C-signal1 | F3 |
| JNK-IN-8 | WM1552C | 2.0000 | uM | 24 | hr | | p27 Kip1 | 257.75 | p-Rb(S807/S811) | 110.40 | p-ERK(T202/Y204) | 269.60 | 2 | WM1552C-signal1 | E3 | |
| Vemurafenib | WM1552C | 0.0000 | uM | 24 | hr | | p27 Kip1 | 243.77 | p-Rb(S807/S811) | 107.70 | p-ERK(T202/Y204) | 254.74 | 1 | WM1552C-signal1 | B11 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 239.37 | p-Rb(S807/S811) | 107.35 | p-ERK(T202/Y204) | 253.29 | 1 | WM1552C-signal1 | F11 |
| Vemurafenib | WM1552C | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 418.63 | p-Rb(S807/S811) | 52.14 | p-ERK(T202/Y204) | 23.96 | 2 | WM1552C-signal1 | G2 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 428.03 | p-Rb(S807/S811) | 51.23 | p-ERK(T202/Y204) | 26.59 | 1 | WM1552C-signal1 | F4 |
| Vemurafenib | WM1552C | 0.1280 | uM | 24 | hr | | p27 Kip1 | 284.96 | p-Rb(S807/S811) | 88.69 | p-ERK(T202/Y204) | 119.29 | 1 | WM1552C-signal1 | B6 | |
| JNK-IN-8 | WM1552C | 0.0033 | uM | 24 | hr | | p27 Kip1 | 241.17 | p-Rb(S807/S811) | 107.27 | p-ERK(T202/Y204) | 256.25 | 2 | WM1552C-signal1 | E10 | |
| Vemurafenib | WM1552C | 0.8000 | uM | 24 | hr | | p27 Kip1 | 440.30 | p-Rb(S807/S811) | 48.95 | p-ERK(T202/Y204) | 28.75 | 2 | WM1552C-signal1 | C4 | |
| JNK-IN-8 | WM1552C | 0.0205 | uM | 24 | hr | | p27 Kip1 | 252.37 | p-Rb(S807/S811) | 110.39 | p-ERK(T202/Y204) | 260.36 | 1 | WM1552C-signal1 | D8 | |
| JNK-IN-8 | WM1552C | 0.0205 | uM | 24 | hr | | p27 Kip1 | 245.39 | p-Rb(S807/S811) | 107.49 | p-ERK(T202/Y204) | 270.03 | 2 | WM1552C-signal1 | E8 | |
| JNK-IN-8 | WM1552C | 0.0082 | uM | 24 | hr | | p27 Kip1 | 239.79 | p-Rb(S807/S811) | 104.64 | p-ERK(T202/Y204) | 258.81 | 1 | WM1552C-signal1 | D9 | |
| Vemurafenib | WM1552C | 0.0082 | uM | 24 | hr | | p27 Kip1 | 242.69 | p-Rb(S807/S811) | 101.68 | p-ERK(T202/Y204) | 236.72 | 1 | WM1552C-signal1 | B9 | |
| Vemurafenib | WM1552C | 0.0512 | uM | 24 | hr | | p27 Kip1 | 262.18 | p-Rb(S807/S811) | 94.16 | p-ERK(T202/Y204) | 164.16 | 2 | WM1552C-signal1 | C7 | |
| Vemurafenib | WM1552C | 2.0000 | uM | 24 | hr | | p27 Kip1 | 473.39 | p-Rb(S807/S811) | 42.61 | p-ERK(T202/Y204) | 22.62 | 1 | WM1552C-signal1 | B3 | |
| Vemurafenib | WM1552C | 5.0000 | uM | 24 | hr | | p27 Kip1 | 433.95 | p-Rb(S807/S811) | 51.26 | p-ERK(T202/Y204) | 22.13 | 2 | WM1552C-signal1 | C2 | |
| Vemurafenib | WM1552C | 0.0205 | uM | 24 | hr | | p27 Kip1 | 249.99 | p-Rb(S807/S811) | 102.20 | p-ERK(T202/Y204) | 212.75 | 2 | WM1552C-signal1 | C8 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 397.69 | p-Rb(S807/S811) | 59.60 | p-ERK(T202/Y204) | 26.88 | 1 | WM1552C-signal1 | F2 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 279.48 | p-Rb(S807/S811) | 87.64 | p-ERK(T202/Y204) | 117.21 | 1 | WM1552C-signal1 | F6 |
| JNK-IN-8 | WM1552C | 5.0000 | uM | 24 | hr | | p27 Kip1 | 304.56 | p-Rb(S807/S811) | 111.24 | p-ERK(T202/Y204) | 317.45 | 1 | WM1552C-signal1 | D2 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 241.65 | p-Rb(S807/S811) | 104.81 | p-ERK(T202/Y204) | 245.66 | 2 | WM1552C-signal1 | G9 |
| JNK-IN-8 | WM1552C | 2.0000 | uM | 24 | hr | | p27 Kip1 | 258.20 | p-Rb(S807/S811) | 113.91 | p-ERK(T202/Y204) | 277.27 | 1 | WM1552C-signal1 | D3 |