Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | WM-115 | 0.0000 | uM | 24 | hr | | Ki-67 | 713.17 | p-Rb(S807/S811) | 74.83 | p-cJun(S73) | 48.37 | 1 | WM115-signal3 | D11 | |
| Vemurafenib | WM-115 | 0.1280 | uM | 24 | hr | | Ki-67 | 771.14 | p-Rb(S807/S811) | 55.68 | p-cJun(S73) | 62.70 | 2 | WM115-signal3 | C6 | |
| Vemurafenib | WM-115 | 0.0205 | uM | 24 | hr | | Ki-67 | 761.10 | p-Rb(S807/S811) | 71.98 | p-cJun(S73) | 49.80 | 2 | WM115-signal3 | C8 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0000 | uM | 24 | hr | | Ki-67 | 782.51 | p-Rb(S807/S811) | 75.83 | p-cJun(S73) | 51.58 | 1 | WM115-signal3 | F11 |
| Vemurafenib | WM-115 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | Ki-67 | 637.76 | p-Rb(S807/S811) | 61.90 | p-cJun(S73) | 19.77 | 2 | WM115-signal3 | G2 |
| Vemurafenib | WM1552C | 5.0000 | uM | 24 | hr | | p27 Kip1 | 446.61 | p-Rb(S807/S811) | 44.76 | p-ERK(T202/Y204) | 19.95 | 1 | WM1552C-signal1 | B2 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 284.31 | p-Rb(S807/S811) | 88.80 | p-ERK(T202/Y204) | 123.78 | 2 | WM1552C-signal1 | G6 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 336.58 | p-Rb(S807/S811) | 69.57 | p-ERK(T202/Y204) | 57.54 | 2 | WM1552C-signal1 | G5 |
| Vemurafenib | WM1552C | 0.0205 | uM | 24 | hr | | p27 Kip1 | 252.92 | p-Rb(S807/S811) | 100.14 | p-ERK(T202/Y204) | 207.93 | 1 | WM1552C-signal1 | B8 | |
| Vemurafenib | WM1552C | 2.0000 | uM | 24 | hr | | p27 Kip1 | 472.02 | p-Rb(S807/S811) | 42.22 | p-ERK(T202/Y204) | 19.71 | 2 | WM1552C-signal1 | C3 | |
| JNK-IN-8 | WM1552C | 5.0000 | uM | 24 | hr | | p27 Kip1 | 313.64 | p-Rb(S807/S811) | 105.04 | p-ERK(T202/Y204) | 304.05 | 2 | WM1552C-signal1 | E2 | |
| JNK-IN-8 | WM1552C | 0.8000 | uM | 24 | hr | | p27 Kip1 | 247.69 | p-Rb(S807/S811) | 115.02 | p-ERK(T202/Y204) | 276.76 | 1 | WM1552C-signal1 | D4 | |
| Vemurafenib | WM1552C | 0.8000 | uM | 24 | hr | | p27 Kip1 | 442.40 | p-Rb(S807/S811) | 51.64 | p-ERK(T202/Y204) | 26.95 | 1 | WM1552C-signal1 | B4 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 231.78 | p-Rb(S807/S811) | 102.69 | p-ERK(T202/Y204) | 234.80 | 1 | WM1552C-signal1 | F10 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 256.65 | p-Rb(S807/S811) | 104.44 | p-ERK(T202/Y204) | 246.25 | 1 | WM1552C-signal1 | F9 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 248.11 | p-Rb(S807/S811) | 100.68 | p-ERK(T202/Y204) | 212.80 | 1 | WM1552C-signal1 | F8 |
| JNK-IN-8 | WM1552C | 0.0000 | uM | 24 | hr | | p27 Kip1 | 244.30 | p-Rb(S807/S811) | 104.67 | p-ERK(T202/Y204) | 252.82 | 1 | WM1552C-signal1 | D11 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 249.58 | p-Rb(S807/S811) | 104.56 | p-ERK(T202/Y204) | 242.42 | 2 | WM1552C-signal1 | G10 |
| Vemurafenib | WM1552C | 0.3200 | uM | 24 | hr | | p27 Kip1 | 349.70 | p-Rb(S807/S811) | 71.79 | p-ERK(T202/Y204) | 64.17 | 2 | WM1552C-signal1 | C5 | |
| Vemurafenib | WM1552C | 0.0082 | uM | 24 | hr | | p27 Kip1 | 241.99 | p-Rb(S807/S811) | 106.90 | p-ERK(T202/Y204) | 245.11 | 2 | WM1552C-signal1 | C9 | |
| Vemurafenib | WM1552C | 0.0033 | uM | 24 | hr | | p27 Kip1 | 240.73 | p-Rb(S807/S811) | 101.89 | p-ERK(T202/Y204) | 242.12 | 2 | WM1552C-signal1 | C10 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 251.50 | p-Rb(S807/S811) | 108.70 | p-ERK(T202/Y204) | 256.82 | 2 | WM1552C-signal1 | G11 |
| JNK-IN-8 | WM1552C | 0.0082 | uM | 24 | hr | | p27 Kip1 | 253.36 | p-Rb(S807/S811) | 110.29 | p-ERK(T202/Y204) | 263.12 | 2 | WM1552C-signal1 | E9 | |
| JNK-IN-8 | WM1552C | 0.0033 | uM | 24 | hr | | p27 Kip1 | 237.48 | p-Rb(S807/S811) | 106.12 | p-ERK(T202/Y204) | 259.42 | 1 | WM1552C-signal1 | D10 | |
| Vemurafenib | WM1552C | 0.1280 | uM | 24 | hr | | p27 Kip1 | 271.43 | p-Rb(S807/S811) | 88.61 | p-ERK(T202/Y204) | 136.09 | 2 | WM1552C-signal1 | C6 |