Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | LOXIMVI | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 265.14 | p-Rb(S807/S811) | 204.08 | p-S6(S235/S236 | 419.00 | 2 | LOXIMVI-signal2 | C8 | |
| JNK-IN-8 | LOXIMVI | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 344.33 | p-Rb(S807/S811) | 248.42 | p-S6(S235/S236 | 524.11 | 1 | LOXIMVI-signal2 | D4 | |
| JNK-IN-8 | LOXIMVI | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 282.86 | p-Rb(S807/S811) | 236.08 | p-S6(S235/S236 | 480.10 | 2 | LOXIMVI-signal2 | E6 | |
| Vemurafenib | LOXIMVI | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 256.68 | p-Rb(S807/S811) | 191.36 | p-S6(S235/S236 | 398.98 | 2 | LOXIMVI-signal2 | C7 | |
| Vemurafenib | LOXIMVI | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 304.49 | p-Rb(S807/S811) | 234.76 | p-S6(S235/S236 | 449.34 | 1 | LOXIMVI-signal2 | B11 | |
| JNK-IN-8 | LOXIMVI | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 291.53 | p-Rb(S807/S811) | 246.99 | p-S6(S235/S236 | 502.86 | 1 | LOXIMVI-signal2 | D5 | |
| Vemurafenib | LOXIMVI | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 290.41 | p-Rb(S807/S811) | 222.56 | p-S6(S235/S236 | 435.26 | 1 | LOXIMVI-signal2 | B10 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 237.24 | p-Rb(S807/S811) | 135.99 | p-S6(S235/S236 | 359.46 | 1 | LOXIMVI-signal2 | F5 |
| JNK-IN-8 | LOXIMVI | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 283.24 | p-Rb(S807/S811) | 237.66 | p-S6(S235/S236 | 456.10 | 1 | LOXIMVI-signal2 | D10 | |
| JNK-IN-8 | LOXIMVI | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 285.05 | p-Rb(S807/S811) | 232.52 | p-S6(S235/S236 | 459.23 | 1 | LOXIMVI-signal2 | D7 | |
| JNK-IN-8 | LOXIMVI | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 254.16 | p-Rb(S807/S811) | 231.99 | p-S6(S235/S236 | 458.18 | 2 | LOXIMVI-signal2 | E9 | |
| JNK-IN-8 | LOXIMVI | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 394.33 | p-Rb(S807/S811) | 290.15 | p-S6(S235/S236 | 433.67 | 2 | LOXIMVI-signal2 | E2 | |
| JNK-IN-8 | LOXIMVI | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 299.94 | p-Rb(S807/S811) | 232.62 | p-S6(S235/S236 | 413.74 | 1 | LOXIMVI-signal2 | D11 | |
| JNK-IN-8 | LOXIMVI | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 260.69 | p-Rb(S807/S811) | 241.93 | p-S6(S235/S236 | 501.67 | 2 | LOXIMVI-signal2 | E5 | |
| JNK-IN-8 | LOXIMVI | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 297.60 | p-Rb(S807/S811) | 258.35 | p-S6(S235/S236 | 550.62 | 2 | LOXIMVI-signal2 | E4 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 306.56 | p-Rb(S807/S811) | 232.04 | p-S6(S235/S236 | 424.09 | 1 | LOXIMVI-signal2 | F11 |
| Vemurafenib | LOXIMVI | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 239.52 | p-Rb(S807/S811) | 154.83 | p-S6(S235/S236 | 375.76 | 1 | LOXIMVI-signal2 | B5 | |
| JNK-IN-8 | LOXIMVI | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 299.84 | p-Rb(S807/S811) | 230.92 | p-S6(S235/S236 | 450.38 | 1 | LOXIMVI-signal2 | D8 | |
| JNK-IN-8 | LOXIMVI | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 368.60 | p-Rb(S807/S811) | 272.58 | p-S6(S235/S236 | 627.74 | 1 | LOXIMVI-signal2 | D3 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 258.76 | p-Rb(S807/S811) | 210.04 | p-S6(S235/S236 | 429.29 | 2 | LOXIMVI-signal2 | G8 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 260.82 | p-Rb(S807/S811) | 220.20 | p-S6(S235/S236 | 459.82 | 2 | LOXIMVI-signal2 | G9 |
| Vemurafenib | LOXIMVI | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 288.44 | p-Rb(S807/S811) | 221.82 | p-S6(S235/S236 | 462.92 | 1 | LOXIMVI-signal2 | B9 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 249.86 | p-Rb(S807/S811) | 181.43 | p-S6(S235/S236 | 413.04 | 1 | LOXIMVI-signal2 | F6 |
| JNK-IN-8 | LOXIMVI | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 281.63 | p-Rb(S807/S811) | 231.63 | p-S6(S235/S236 | 455.97 | 2 | LOXIMVI-signal2 | E8 | |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 218.07 | p-Rb(S807/S811) | 73.50 | p-S6(S235/S236 | 96.41 | 2 | LOXIMVI-signal2 | C2 |