Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | LOXIMVI | 0.1280 | uM | 24 | hr | | p27 Kip1 | 366.51 | p-Rb(S807/S811) | 180.61 | p-ERK(T202/Y204) | 84.29 | 1 | LOXIMVI-signal1 | B6 | |
| Vemurafenib | LOXIMVI | 0.0033 | uM | 24 | hr | | p27 Kip1 | 339.87 | p-Rb(S807/S811) | 207.46 | p-ERK(T202/Y204) | 188.18 | 1 | LOXIMVI-signal1 | B10 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 351.99 | p-Rb(S807/S811) | 215.31 | p-ERK(T202/Y204) | 217.61 | 2 | LOXIMVI-signal1 | G11 |
| JNK-IN-8 | LOXIMVI | 0.0512 | uM | 24 | hr | | p27 Kip1 | 316.14 | p-Rb(S807/S811) | 216.98 | p-ERK(T202/Y204) | 207.82 | 1 | LOXIMVI-signal1 | D7 | |
| Vemurafenib | LOXIMVI | 0.0000 | uM | 24 | hr | | p27 Kip1 | 346.49 | p-Rb(S807/S811) | 215.22 | p-ERK(T202/Y204) | 215.11 | 1 | LOXIMVI-signal1 | B11 | |
| Vemurafenib | LOXIMVI | 2.0000 | uM | 24 | hr | | p27 Kip1 | 456.75 | p-Rb(S807/S811) | 94.75 | p-ERK(T202/Y204) | 28.87 | 1 | LOXIMVI-signal1 | B3 | |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | p27 Kip1 | 487.55 | p-Rb(S807/S811) | 84.67 | p-ERK(T202/Y204) | 31.63 | 2 | LOXIMVI-signal1 | C2 | |
| Vemurafenib | LOXIMVI | 0.0512 | uM | 24 | hr | | p27 Kip1 | 344.62 | p-Rb(S807/S811) | 191.81 | p-ERK(T202/Y204) | 94.25 | 1 | LOXIMVI-signal1 | B7 | |
| JNK-IN-8 | LOXIMVI | 5.0000 | uM | 24 | hr | | p27 Kip1 | 472.18 | p-Rb(S807/S811) | 278.69 | p-ERK(T202/Y204) | 272.54 | 2 | LOXIMVI-signal1 | E2 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 609.38 | p-Rb(S807/S811) | 99.97 | p-ERK(T202/Y204) | 70.02 | 2 | LOXIMVI-signal1 | G2 |
| Vemurafenib | LOXIMVI | 0.0512 | uM | 24 | hr | | p27 Kip1 | 346.34 | p-Rb(S807/S811) | 184.23 | p-ERK(T202/Y204) | 94.79 | 2 | LOXIMVI-signal1 | C7 | |
| Vemurafenib | LOXIMVI | 0.8000 | uM | 24 | hr | | p27 Kip1 | 437.65 | p-Rb(S807/S811) | 139.21 | p-ERK(T202/Y204) | 46.29 | 1 | LOXIMVI-signal1 | B4 | |
| JNK-IN-8 | LOXIMVI | 0.0082 | uM | 24 | hr | | p27 Kip1 | 307.33 | p-Rb(S807/S811) | 208.17 | p-ERK(T202/Y204) | 200.97 | 1 | LOXIMVI-signal1 | D9 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 356.71 | p-Rb(S807/S811) | 166.33 | p-ERK(T202/Y204) | 71.63 | 1 | LOXIMVI-signal1 | F6 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 307.03 | p-Rb(S807/S811) | 198.87 | p-ERK(T202/Y204) | 175.67 | 1 | LOXIMVI-signal1 | F10 |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 210.99 | p-Rb(S807/S811) | 80.53 | p-S6(S235/S236 | 102.92 | 1 | LOXIMVI-signal2 | B2 | |
| Vemurafenib | LOXIMVI | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 280.25 | p-Rb(S807/S811) | 186.93 | p-S6(S235/S236 | 402.53 | 2 | LOXIMVI-signal2 | C6 | |
| Vemurafenib | LOXIMVI | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 318.16 | p-Rb(S807/S811) | 230.07 | p-S6(S235/S236 | 423.94 | 2 | LOXIMVI-signal2 | C11 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 325.81 | p-Rb(S807/S811) | 222.34 | p-S6(S235/S236 | 438.90 | 2 | LOXIMVI-signal2 | G11 |
| Vemurafenib | LOXIMVI | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 248.35 | p-Rb(S807/S811) | 129.91 | p-S6(S235/S236 | 357.36 | 2 | LOXIMVI-signal2 | C4 | |
| Vemurafenib | LOXIMVI | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 282.64 | p-Rb(S807/S811) | 182.20 | p-S6(S235/S236 | 400.81 | 1 | LOXIMVI-signal2 | B6 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 231.93 | p-Rb(S807/S811) | 91.17 | p-S6(S235/S236 | 89.76 | 2 | LOXIMVI-signal2 | G3 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 258.89 | p-Rb(S807/S811) | 123.29 | p-S6(S235/S236 | 272.97 | 2 | LOXIMVI-signal2 | G4 |
| Vemurafenib | LOXIMVI | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 255.47 | p-Rb(S807/S811) | 192.91 | p-S6(S235/S236 | 440.25 | 1 | LOXIMVI-signal2 | B7 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 269.36 | p-Rb(S807/S811) | 224.35 | p-S6(S235/S236 | 447.46 | 1 | LOXIMVI-signal2 | F10 |