Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | LOXIMVI | 0.1280 | uM | 24 | hr | | p27 Kip1 | 297.55 | p-Rb(S807/S811) | 211.55 | p-ERK(T202/Y204) | 210.25 | 2 | LOXIMVI-signal1 | E6 | |
| JNK-IN-8 | LOXIMVI | 0.3200 | uM | 24 | hr | | p27 Kip1 | 306.81 | p-Rb(S807/S811) | 215.56 | p-ERK(T202/Y204) | 200.77 | 2 | LOXIMVI-signal1 | E5 | |
| JNK-IN-8 | COLO 858 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 450.18 | p-Rb(S807/S811) | 173.59 | p-ERK(T202/Y204) | 691.16 | 1 | Colo858-signal1 | D6 | |
| JNK-IN-8 | LOXIMVI | 0.8000 | uM | 24 | hr | | p27 Kip1 | 330.87 | p-Rb(S807/S811) | 223.53 | p-ERK(T202/Y204) | 212.37 | 2 | LOXIMVI-signal1 | E4 | |
| JNK-IN-8 | LOXIMVI | 2.0000 | uM | 24 | hr | | p27 Kip1 | 368.98 | p-Rb(S807/S811) | 251.60 | p-ERK(T202/Y204) | 236.95 | 2 | LOXIMVI-signal1 | E3 | |
| JNK-IN-8 | LOXIMVI | 5.0000 | uM | 24 | hr | | p27 Kip1 | 472.18 | p-Rb(S807/S811) | 278.69 | p-ERK(T202/Y204) | 272.54 | 2 | LOXIMVI-signal1 | E2 | |
| JNK-IN-8 | LOXIMVI | 0.0000 | uM | 24 | hr | | p27 Kip1 | 351.82 | p-Rb(S807/S811) | 222.97 | p-ERK(T202/Y204) | 225.84 | 2 | LOXIMVI-signal1 | E11 | |
| JNK-IN-8 | LOXIMVI | 0.0033 | uM | 24 | hr | | p27 Kip1 | 293.36 | p-Rb(S807/S811) | 201.67 | p-ERK(T202/Y204) | 200.98 | 2 | LOXIMVI-signal1 | E10 | |
| JNK-IN-8 | LOXIMVI | 0.0082 | uM | 24 | hr | | p27 Kip1 | 307.33 | p-Rb(S807/S811) | 208.17 | p-ERK(T202/Y204) | 200.97 | 1 | LOXIMVI-signal1 | D9 | |
| JNK-IN-8 | LOXIMVI | 0.0205 | uM | 24 | hr | | p27 Kip1 | 303.56 | p-Rb(S807/S811) | 209.54 | p-ERK(T202/Y204) | 206.46 | 1 | LOXIMVI-signal1 | D8 | |
| JNK-IN-8 | LOXIMVI | 0.0512 | uM | 24 | hr | | p27 Kip1 | 316.14 | p-Rb(S807/S811) | 216.98 | p-ERK(T202/Y204) | 207.82 | 1 | LOXIMVI-signal1 | D7 | |
| JNK-IN-8 | COLO 858 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 453.72 | p-Rb(S807/S811) | 173.99 | p-ERK(T202/Y204) | 686.67 | 1 | Colo858-signal1 | D5 | |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 1100.60 | p-Rb(S807/S811) | 49.89 | p-ERK(T202/Y204) | 9.41 | 2 | Colo858-signal1 | C2 | |
| JNK-IN-8 | LOXIMVI | 0.1280 | uM | 24 | hr | | p27 Kip1 | 319.20 | p-Rb(S807/S811) | 216.66 | p-ERK(T202/Y204) | 220.23 | 1 | LOXIMVI-signal1 | D6 | |
| JNK-IN-8 | LOXIMVI | 0.3200 | uM | 24 | hr | | p27 Kip1 | 312.28 | p-Rb(S807/S811) | 224.98 | p-ERK(T202/Y204) | 217.75 | 1 | LOXIMVI-signal1 | D5 | |
| JNK-IN-8 | LOXIMVI | 0.8000 | uM | 24 | hr | | p27 Kip1 | 336.96 | p-Rb(S807/S811) | 231.40 | p-ERK(T202/Y204) | 222.11 | 1 | LOXIMVI-signal1 | D4 | |
| JNK-IN-8 | LOXIMVI | 2.0000 | uM | 24 | hr | | p27 Kip1 | 376.92 | p-Rb(S807/S811) | 258.15 | p-ERK(T202/Y204) | 235.58 | 1 | LOXIMVI-signal1 | D3 | |
| JNK-IN-8 | LOXIMVI | 5.0000 | uM | 24 | hr | | p27 Kip1 | 464.53 | p-Rb(S807/S811) | 268.11 | p-ERK(T202/Y204) | 267.97 | 1 | LOXIMVI-signal1 | D2 | |
| JNK-IN-8 | LOXIMVI | 0.0000 | uM | 24 | hr | | p27 Kip1 | 348.21 | p-Rb(S807/S811) | 217.92 | p-ERK(T202/Y204) | 232.50 | 1 | LOXIMVI-signal1 | D11 | |
| JNK-IN-8 | LOXIMVI | 0.0033 | uM | 24 | hr | | p27 Kip1 | 307.47 | p-Rb(S807/S811) | 207.98 | p-ERK(T202/Y204) | 211.16 | 1 | LOXIMVI-signal1 | D10 | |
| Vemurafenib | LOXIMVI | 0.0082 | uM | 24 | hr | | p27 Kip1 | 317.72 | p-Rb(S807/S811) | 203.25 | p-ERK(T202/Y204) | 167.85 | 2 | LOXIMVI-signal1 | C9 | |
| JNK-IN-8 | COLO 858 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 373.77 | p-Rb(S807/S811) | 163.58 | p-S6(S235/S236 | 386.62 | 2 | Colo858-signal2 | E9 | |
| JNK-IN-8 | COLO 858 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 488.74 | p-Rb(S807/S811) | 190.14 | p-ERK(T202/Y204) | 694.83 | 1 | Colo858-signal1 | D4 | |
| Vemurafenib | LOXIMVI | 0.0205 | uM | 24 | hr | | p27 Kip1 | 319.35 | p-Rb(S807/S811) | 195.10 | p-ERK(T202/Y204) | 133.04 | 2 | LOXIMVI-signal1 | C8 | |
| Vemurafenib | LOXIMVI | 0.0512 | uM | 24 | hr | | p27 Kip1 | 346.34 | p-Rb(S807/S811) | 184.23 | p-ERK(T202/Y204) | 94.79 | 2 | LOXIMVI-signal1 | C7 |