Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | LOXIMVI | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 318.16 | p-Rb(S807/S811) | 230.07 | p-S6(S235/S236 | 423.94 | 2 | LOXIMVI-signal2 | C11 | |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 218.07 | p-Rb(S807/S811) | 73.50 | p-S6(S235/S236 | 96.41 | 2 | LOXIMVI-signal2 | C2 | |
| Vemurafenib | LOXIMVI | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 184.72 | p-Rb(S807/S811) | 84.06 | p-S6(S235/S236 | 180.02 | 2 | LOXIMVI-signal2 | C3 | |
| Vemurafenib | LOXIMVI | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 248.35 | p-Rb(S807/S811) | 129.91 | p-S6(S235/S236 | 357.36 | 2 | LOXIMVI-signal2 | C4 | |
| JNK-IN-8 | COLO 858 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 442.59 | p-Rb(S807/S811) | 160.97 | p-ERK(T202/Y204) | 615.32 | 2 | Colo858-signal1 | E10 | |
| Vemurafenib | LOXIMVI | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 282.64 | p-Rb(S807/S811) | 182.20 | p-S6(S235/S236 | 400.81 | 1 | LOXIMVI-signal2 | B6 | |
| Vemurafenib | LOXIMVI | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 239.52 | p-Rb(S807/S811) | 154.83 | p-S6(S235/S236 | 375.76 | 1 | LOXIMVI-signal2 | B5 | |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | Ki-67 | 801.46 | p-Rb(S807/S811) | 44.01 | p-cJun(S73) | 45.24 | 1 | Colo858-signal3 | B2 | |
| Vemurafenib | LOXIMVI | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 237.39 | p-Rb(S807/S811) | 127.65 | p-S6(S235/S236 | 339.13 | 1 | LOXIMVI-signal2 | B4 | |
| Vemurafenib | LOXIMVI | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 203.52 | p-Rb(S807/S811) | 97.55 | p-S6(S235/S236 | 181.29 | 1 | LOXIMVI-signal2 | B3 | |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | Ki-67 | 2209.00 | p-Rb(S807/S811) | 151.07 | p-cJun(S73) | 28.62 | 1 | Colo858-signal3 | B11 | |
| Vemurafenib | COLO 858 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 326.58 | p-Rb(S807/S811) | 162.30 | p-S6(S235/S236 | 402.84 | 1 | Colo858-signal2 | B10 | |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 210.99 | p-Rb(S807/S811) | 80.53 | p-S6(S235/S236 | 102.92 | 1 | LOXIMVI-signal2 | B2 | |
| Vemurafenib | LOXIMVI | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 304.49 | p-Rb(S807/S811) | 234.76 | p-S6(S235/S236 | 449.34 | 1 | LOXIMVI-signal2 | B11 | |
| Vemurafenib | LOXIMVI | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 290.41 | p-Rb(S807/S811) | 222.56 | p-S6(S235/S236 | 435.26 | 1 | LOXIMVI-signal2 | B10 | |
| Vemurafenib | COLO 858 | 0.0033 | uM | 24 | hr | | Ki-67 | 2154.80 | p-Rb(S807/S811) | 145.55 | p-cJun(S73) | 28.64 | 1 | Colo858-signal3 | B10 | |
| JNK-IN-8 | COLO 858 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 436.36 | p-Rb(S807/S811) | 155.67 | p-ERK(T202/Y204) | 648.15 | 1 | Colo858-signal1 | D9 | |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 1014.60 | p-Rb(S807/S811) | 48.01 | p-ERK(T202/Y204) | 18.67 | 2 | Colo858-signal1 | C4 | |
| JNK-IN-8 | COLO 858 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 439.43 | p-Rb(S807/S811) | 169.34 | p-ERK(T202/Y204) | 657.25 | 1 | Colo858-signal1 | D8 | |
| JNK-IN-8 | COLO 858 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 430.28 | p-Rb(S807/S811) | 171.77 | p-ERK(T202/Y204) | 673.40 | 1 | Colo858-signal1 | D7 | |
| Vemurafenib | COLO 858 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 1027.10 | p-Rb(S807/S811) | 46.02 | p-ERK(T202/Y204) | 14.33 | 2 | Colo858-signal1 | C3 | |
| Vemurafenib | COLO 858 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 849.44 | p-Rb(S807/S811) | 79.57 | p-ERK(T202/Y204) | 75.64 | 1 | Colo858-signal1 | B6 | |
| JNK-IN-8 | LOXIMVI | 0.0082 | uM | 24 | hr | | p27 Kip1 | 300.54 | p-Rb(S807/S811) | 201.98 | p-ERK(T202/Y204) | 205.87 | 2 | LOXIMVI-signal1 | E9 | |
| JNK-IN-8 | LOXIMVI | 0.0205 | uM | 24 | hr | | p27 Kip1 | 286.32 | p-Rb(S807/S811) | 204.47 | p-ERK(T202/Y204) | 198.65 | 2 | LOXIMVI-signal1 | E8 | |
| JNK-IN-8 | LOXIMVI | 0.0512 | uM | 24 | hr | | p27 Kip1 | 303.12 | p-Rb(S807/S811) | 207.96 | p-ERK(T202/Y204) | 204.10 | 2 | LOXIMVI-signal1 | E7 |