Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | LOXIMVI | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 318.16 | p-Rb(S807/S811) | 230.07 | p-S6(S235/S236 | 423.94 | 2 | LOXIMVI-signal2 | C11 | |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 218.07 | p-Rb(S807/S811) | 73.50 | p-S6(S235/S236 | 96.41 | 2 | LOXIMVI-signal2 | C2 | |
| Vemurafenib | LOXIMVI | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 184.72 | p-Rb(S807/S811) | 84.06 | p-S6(S235/S236 | 180.02 | 2 | LOXIMVI-signal2 | C3 | |
| Vemurafenib | LOXIMVI | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 248.35 | p-Rb(S807/S811) | 129.91 | p-S6(S235/S236 | 357.36 | 2 | LOXIMVI-signal2 | C4 | |
| Vemurafenib | LOXIMVI | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 249.36 | p-Rb(S807/S811) | 148.83 | p-S6(S235/S236 | 383.67 | 2 | LOXIMVI-signal2 | C5 | |
| Vemurafenib | LOXIMVI | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 280.25 | p-Rb(S807/S811) | 186.93 | p-S6(S235/S236 | 402.53 | 2 | LOXIMVI-signal2 | C6 | |
| Vemurafenib | LOXIMVI | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 256.68 | p-Rb(S807/S811) | 191.36 | p-S6(S235/S236 | 398.98 | 2 | LOXIMVI-signal2 | C7 | |
| Vemurafenib | LOXIMVI | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 265.14 | p-Rb(S807/S811) | 204.08 | p-S6(S235/S236 | 419.00 | 2 | LOXIMVI-signal2 | C8 | |
| Vemurafenib | LOXIMVI | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 264.06 | p-Rb(S807/S811) | 220.85 | p-S6(S235/S236 | 434.18 | 2 | LOXIMVI-signal2 | C9 | |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 1028.10 | p-Rb(S807/S811) | 48.93 | p-ERK(T202/Y204) | 27.49 | 2 | Colo858-signal1 | C5 | |
| Vemurafenib | COLO 858 | 0.0033 | uM | 24 | hr | | Ki-67 | 2240.50 | p-Rb(S807/S811) | 144.95 | p-cJun(S73) | 28.17 | 2 | Colo858-signal3 | C10 | |
| JNK-IN-8 | LOXIMVI | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 281.63 | p-Rb(S807/S811) | 231.63 | p-S6(S235/S236 | 455.97 | 2 | LOXIMVI-signal2 | E8 | |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | Ki-67 | 2259.80 | p-Rb(S807/S811) | 154.64 | p-cJun(S73) | 29.83 | 2 | Colo858-signal3 | C11 | |
| JNK-IN-8 | COLO 858 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 839.15 | p-Rb(S807/S811) | 256.43 | p-ERK(T202/Y204) | 789.95 | 2 | Colo858-signal1 | E2 | |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | Ki-67 | 947.93 | p-Rb(S807/S811) | 51.31 | p-cJun(S73) | 41.01 | 2 | Colo858-signal3 | C2 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 231.93 | p-Rb(S807/S811) | 91.17 | p-S6(S235/S236 | 89.76 | 2 | LOXIMVI-signal2 | G3 |
| Vemurafenib | COLO 858 | 2.0000 | uM | 24 | hr | | Ki-67 | 782.90 | p-Rb(S807/S811) | 45.43 | p-cJun(S73) | 59.46 | 2 | Colo858-signal3 | C3 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 268.49 | p-Rb(S807/S811) | 170.62 | p-S6(S235/S236 | 412.34 | 2 | LOXIMVI-signal2 | G6 |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | Ki-67 | 680.51 | p-Rb(S807/S811) | 40.34 | p-cJun(S73) | 71.07 | 2 | Colo858-signal3 | C4 | |
| JNK-IN-8 | LOXIMVI | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 242.93 | p-Rb(S807/S811) | 227.00 | p-S6(S235/S236 | 449.29 | 2 | LOXIMVI-signal2 | E10 | |
| JNK-IN-8 | LOXIMVI | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 312.66 | p-Rb(S807/S811) | 236.29 | p-S6(S235/S236 | 442.43 | 2 | LOXIMVI-signal2 | E11 | |
| JNK-IN-8 | LOXIMVI | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 394.33 | p-Rb(S807/S811) | 290.15 | p-S6(S235/S236 | 433.67 | 2 | LOXIMVI-signal2 | E2 | |
| JNK-IN-8 | LOXIMVI | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 377.73 | p-Rb(S807/S811) | 274.50 | p-S6(S235/S236 | 619.52 | 2 | LOXIMVI-signal2 | E3 | |
| Vemurafenib | WM1552C | 0.8000 | uM | 24 | hr | | p27 Kip1 | 440.30 | p-Rb(S807/S811) | 48.95 | p-ERK(T202/Y204) | 28.75 | 2 | WM1552C-signal1 | C4 | |
| Vemurafenib | WM1552C | 2.0000 | uM | 24 | hr | | p27 Kip1 | 472.02 | p-Rb(S807/S811) | 42.22 | p-ERK(T202/Y204) | 19.71 | 2 | WM1552C-signal1 | C3 |