Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | Ki-67 | 2138.80 | p-Rb(S807/S811) | 144.45 | p-cJun(S73) | 30.43 | 2 | Colo858-signal3 | C8 | |
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 483.48 | p-Rb(S807/S811) | 154.86 | p-ERK(T202/Y204) | 515.79 | 2 | Colo858-signal1 | C8 | |
| Vemurafenib | WM1552C | 0.0205 | uM | 24 | hr | | p27 Kip1 | 249.99 | p-Rb(S807/S811) | 102.20 | p-ERK(T202/Y204) | 212.75 | 2 | WM1552C-signal1 | C8 | |
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 349.40 | p-Rb(S807/S811) | 166.03 | p-S6(S235/S236 | 385.58 | 2 | Colo858-signal2 | C8 | |
| Vemurafenib | WM-115 | 0.0512 | uM | 24 | hr | | Ki-67 | 678.09 | p-Rb(S807/S811) | 65.24 | p-cJun(S73) | 51.35 | 2 | WM115-signal3 | C7 | |
| Vemurafenib | LOXIMVI | 0.0512 | uM | 24 | hr | | Ki-67 | 2629.00 | p-Rb(S807/S811) | 170.98 | p-cJun(S73) | 89.13 | 2 | LOXIMVI-signal3 | C7 | |
| Vemurafenib | COLO 858 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 274.60 | p-Rb(S807/S811) | 140.30 | p-S6(S235/S236 | 340.20 | 2 | Colo858-signal2 | C7 | |
| Vemurafenib | WM1552C | 0.0512 | uM | 24 | hr | | p27 Kip1 | 262.18 | p-Rb(S807/S811) | 94.16 | p-ERK(T202/Y204) | 164.16 | 2 | WM1552C-signal1 | C7 | |
| Vemurafenib | LOXIMVI | 0.0512 | uM | 24 | hr | | p27 Kip1 | 346.34 | p-Rb(S807/S811) | 184.23 | p-ERK(T202/Y204) | 94.79 | 2 | LOXIMVI-signal1 | C7 | |
| Vemurafenib | WM-115 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 238.56 | p-Rb(S807/S811) | 70.44 | p-ERK(T202/Y204) | 385.55 | 2 | WM115-signal1 | C7 | |
| Vemurafenib | LOXIMVI | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 256.68 | p-Rb(S807/S811) | 191.36 | p-S6(S235/S236 | 398.98 | 2 | LOXIMVI-signal2 | C7 | |
| Vemurafenib | WM1552C | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 224.64 | p-Rb(S807/S811) | 95.99 | p-S6(S235/S236 | 373.81 | 2 | WM1552C-signal2 | C7 | |
| Vemurafenib | COLO 858 | 0.0512 | uM | 24 | hr | | Ki-67 | 1637.20 | p-Rb(S807/S811) | 124.19 | p-cJun(S73) | 36.36 | 2 | Colo858-signal3 | C7 | |
| Vemurafenib | COLO 858 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 597.47 | p-Rb(S807/S811) | 132.94 | p-ERK(T202/Y204) | 291.40 | 2 | Colo858-signal1 | C7 | |
| Vemurafenib | WM1552C | 0.0512 | uM | 24 | hr | | Ki-67 | 1417.80 | p-Rb(S807/S811) | 90.84 | p-cJun(S73) | 153.01 | 2 | WM1552C-signal3 | C7 | |
| Vemurafenib | WM-115 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 217.58 | p-Rb(S807/S811) | 65.73 | p-S6(S235/S236 | 171.08 | 2 | WM115-signal2 | C7 | |
| Vemurafenib | WM-115 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 235.53 | p-Rb(S807/S811) | 54.90 | p-ERK(T202/Y204) | 152.31 | 2 | WM115-signal1 | C6 | |
| Vemurafenib | COLO 858 | 0.1280 | uM | 24 | hr | | Ki-67 | 1050.90 | p-Rb(S807/S811) | 82.58 | p-cJun(S73) | 62.66 | 2 | Colo858-signal3 | C6 | |
| Vemurafenib | WM-115 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 201.09 | p-Rb(S807/S811) | 53.27 | p-S6(S235/S236 | 128.83 | 2 | WM115-signal2 | C6 | |
| Vemurafenib | WM1552C | 0.1280 | uM | 24 | hr | | p27 Kip1 | 271.43 | p-Rb(S807/S811) | 88.61 | p-ERK(T202/Y204) | 136.09 | 2 | WM1552C-signal1 | C6 | |
| Vemurafenib | LOXIMVI | 0.1280 | uM | 24 | hr | | Ki-67 | 2570.00 | p-Rb(S807/S811) | 157.19 | p-cJun(S73) | 85.99 | 2 | LOXIMVI-signal3 | C6 | |
| Vemurafenib | WM1552C | 0.1280 | uM | 24 | hr | | Ki-67 | 1348.40 | p-Rb(S807/S811) | 86.19 | p-cJun(S73) | 162.31 | 2 | WM1552C-signal3 | C6 | |
| Vemurafenib | WM-115 | 0.1280 | uM | 24 | hr | | Ki-67 | 771.14 | p-Rb(S807/S811) | 55.68 | p-cJun(S73) | 62.70 | 2 | WM115-signal3 | C6 | |
| Vemurafenib | LOXIMVI | 0.1280 | uM | 24 | hr | | p27 Kip1 | 364.85 | p-Rb(S807/S811) | 178.03 | p-ERK(T202/Y204) | 76.08 | 2 | LOXIMVI-signal1 | C6 | |
| Vemurafenib | COLO 858 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 904.25 | p-Rb(S807/S811) | 65.93 | p-ERK(T202/Y204) | 58.28 | 2 | Colo858-signal1 | C6 |