Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | WM-115 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 237.86 | p-Rb(S807/S811) | 81.40 | p-ERK(T202/Y204) | 668.60 | 2 | WM115-signal1 | E7 | |
| JNK-IN-8 | WM1552C | 0.1280 | uM | 24 | hr | | p27 Kip1 | 230.98 | p-Rb(S807/S811) | 107.78 | p-ERK(T202/Y204) | 265.42 | 2 | WM1552C-signal1 | E6 | |
| JNK-IN-8 | LOXIMVI | 0.1280 | uM | 24 | hr | | p27 Kip1 | 297.55 | p-Rb(S807/S811) | 211.55 | p-ERK(T202/Y204) | 210.25 | 2 | LOXIMVI-signal1 | E6 | |
| JNK-IN-8 | WM1552C | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 233.60 | p-Rb(S807/S811) | 115.43 | p-S6(S235/S236 | 404.08 | 2 | WM1552C-signal2 | E6 | |
| JNK-IN-8 | WM-115 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 235.79 | p-Rb(S807/S811) | 81.08 | p-ERK(T202/Y204) | 651.65 | 2 | WM115-signal1 | E6 | |
| JNK-IN-8 | COLO 858 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 370.36 | p-Rb(S807/S811) | 181.15 | p-S6(S235/S236 | 424.43 | 2 | Colo858-signal2 | E6 | |
| JNK-IN-8 | LOXIMVI | 0.1280 | uM | 24 | hr | | Ki-67 | 2886.70 | p-Rb(S807/S811) | 203.46 | p-cJun(S73) | 63.66 | 2 | LOXIMVI-signal3 | E6 | |
| JNK-IN-8 | WM-115 | 0.1280 | uM | 24 | hr | | Ki-67 | 748.11 | p-Rb(S807/S811) | 75.71 | p-cJun(S73) | 27.81 | 2 | WM115-signal3 | E6 | |
| JNK-IN-8 | COLO 858 | 0.1280 | uM | 24 | hr | | Ki-67 | 2233.20 | p-Rb(S807/S811) | 162.30 | p-cJun(S73) | 29.52 | 2 | Colo858-signal3 | E6 | |
| JNK-IN-8 | COLO 858 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 429.89 | p-Rb(S807/S811) | 170.46 | p-ERK(T202/Y204) | 675.31 | 2 | Colo858-signal1 | E6 | |
| JNK-IN-8 | WM1552C | 0.1280 | uM | 24 | hr | | Ki-67 | 1589.50 | p-Rb(S807/S811) | 107.97 | p-cJun(S73) | 133.56 | 2 | WM1552C-signal3 | E6 | |
| JNK-IN-8 | LOXIMVI | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 282.86 | p-Rb(S807/S811) | 236.08 | p-S6(S235/S236 | 480.10 | 2 | LOXIMVI-signal2 | E6 | |
| JNK-IN-8 | WM-115 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 243.03 | p-Rb(S807/S811) | 77.65 | p-S6(S235/S236 | 178.01 | 2 | WM115-signal2 | E6 | |
| JNK-IN-8 | WM1552C | 0.3200 | uM | 24 | hr | | p27 Kip1 | 238.39 | p-Rb(S807/S811) | 107.84 | p-ERK(T202/Y204) | 264.88 | 2 | WM1552C-signal1 | E5 | |
| JNK-IN-8 | WM-115 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 232.47 | p-Rb(S807/S811) | 84.23 | p-ERK(T202/Y204) | 633.23 | 2 | WM115-signal1 | E5 | |
| JNK-IN-8 | LOXIMVI | 0.3200 | uM | 24 | hr | | p27 Kip1 | 306.81 | p-Rb(S807/S811) | 215.56 | p-ERK(T202/Y204) | 200.77 | 2 | LOXIMVI-signal1 | E5 | |
| JNK-IN-8 | COLO 858 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 302.44 | p-Rb(S807/S811) | 181.09 | p-S6(S235/S236 | 433.45 | 2 | Colo858-signal2 | E5 | |
| JNK-IN-8 | LOXIMVI | 0.3200 | uM | 24 | hr | | Ki-67 | 2848.50 | p-Rb(S807/S811) | 207.66 | p-cJun(S73) | 57.32 | 2 | LOXIMVI-signal3 | E5 | |
| JNK-IN-8 | WM-115 | 0.3200 | uM | 24 | hr | | Ki-67 | 785.96 | p-Rb(S807/S811) | 71.52 | p-cJun(S73) | 21.74 | 2 | WM115-signal3 | E5 | |
| JNK-IN-8 | WM-115 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 260.22 | p-Rb(S807/S811) | 76.23 | p-S6(S235/S236 | 189.51 | 2 | WM115-signal2 | E5 | |
| JNK-IN-8 | COLO 858 | 0.3200 | uM | 24 | hr | | Ki-67 | 2300.70 | p-Rb(S807/S811) | 172.32 | p-cJun(S73) | 30.64 | 2 | Colo858-signal3 | E5 | |
| JNK-IN-8 | WM1552C | 0.3200 | uM | 24 | hr | | Ki-67 | 1603.10 | p-Rb(S807/S811) | 108.59 | p-cJun(S73) | 104.20 | 2 | WM1552C-signal3 | E5 | |
| JNK-IN-8 | COLO 858 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 445.16 | p-Rb(S807/S811) | 182.28 | p-ERK(T202/Y204) | 673.10 | 2 | Colo858-signal1 | E5 | |
| JNK-IN-8 | LOXIMVI | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 260.69 | p-Rb(S807/S811) | 241.93 | p-S6(S235/S236 | 501.67 | 2 | LOXIMVI-signal2 | E5 | |
| JNK-IN-8 | WM1552C | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 238.67 | p-Rb(S807/S811) | 112.35 | p-S6(S235/S236 | 403.49 | 2 | WM1552C-signal2 | E5 |