Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | Ki-67 | 1608.40 | p-Rb(S807/S811) | 136.81 | p-cJun(S73) | 31.60 | 2 | Colo858-signal3 | G7 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 233.71 | p-Rb(S807/S811) | 136.67 | p-S6(S235/S236 | 375.36 | 2 | LOXIMVI-signal2 | G5 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 237.24 | p-Rb(S807/S811) | 135.99 | p-S6(S235/S236 | 359.46 | 1 | LOXIMVI-signal2 | F5 |
| Vemurafenib | LOXIMVI | 0.3200 | uM | 24 | hr | | Ki-67 | 2387.10 | p-Rb(S807/S811) | 134.09 | p-cJun(S73) | 83.10 | 2 | LOXIMVI-signal3 | C5 | |
| Vemurafenib | COLO 858 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 597.47 | p-Rb(S807/S811) | 132.94 | p-ERK(T202/Y204) | 291.40 | 2 | Colo858-signal1 | C7 | |
| Vemurafenib | LOXIMVI | 0.8000 | uM | 24 | hr | | p27 Kip1 | 434.48 | p-Rb(S807/S811) | 132.48 | p-ERK(T202/Y204) | 43.96 | 2 | LOXIMVI-signal1 | C4 | |
| Vemurafenib | LOXIMVI | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 248.35 | p-Rb(S807/S811) | 129.91 | p-S6(S235/S236 | 357.36 | 2 | LOXIMVI-signal2 | C4 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | Ki-67 | 1684.50 | p-Rb(S807/S811) | 129.01 | p-cJun(S73) | 29.91 | 1 | Colo858-signal3 | F7 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 525.79 | p-Rb(S807/S811) | 128.17 | p-S6(S235/S236 | 40.46 | 2 | Colo858-signal2 | G2 |
| Vemurafenib | LOXIMVI | 0.8000 | uM | 24 | hr | | Ki-67 | 2357.40 | p-Rb(S807/S811) | 128.12 | p-cJun(S73) | 82.54 | 1 | LOXIMVI-signal3 | B4 | |
| Vemurafenib | LOXIMVI | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 237.39 | p-Rb(S807/S811) | 127.65 | p-S6(S235/S236 | 339.13 | 1 | LOXIMVI-signal2 | B4 | |
| Vemurafenib | LOXIMVI | 0.8000 | uM | 24 | hr | | Ki-67 | 2448.30 | p-Rb(S807/S811) | 126.79 | p-cJun(S73) | 81.56 | 2 | LOXIMVI-signal3 | C4 | |
| Vemurafenib | COLO 858 | 0.0512 | uM | 24 | hr | | Ki-67 | 1742.30 | p-Rb(S807/S811) | 126.75 | p-cJun(S73) | 38.16 | 1 | Colo858-signal3 | B7 | |
| Vemurafenib | COLO 858 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 555.61 | p-Rb(S807/S811) | 125.96 | p-ERK(T202/Y204) | 272.64 | 1 | Colo858-signal1 | B7 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 537.76 | p-Rb(S807/S811) | 125.84 | p-ERK(T202/Y204) | 268.22 | 1 | Colo858-signal1 | F7 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 441.68 | p-Rb(S807/S811) | 125.56 | p-ERK(T202/Y204) | 43.30 | 1 | LOXIMVI-signal1 | F5 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 511.00 | p-Rb(S807/S811) | 125.23 | p-ERK(T202/Y204) | 254.95 | 2 | Colo858-signal1 | G7 |
| Vemurafenib | COLO 858 | 0.0512 | uM | 24 | hr | | Ki-67 | 1637.20 | p-Rb(S807/S811) | 124.19 | p-cJun(S73) | 36.36 | 2 | Colo858-signal3 | C7 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 432.08 | p-Rb(S807/S811) | 123.48 | p-ERK(T202/Y204) | 43.03 | 2 | LOXIMVI-signal1 | G5 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 258.89 | p-Rb(S807/S811) | 123.29 | p-S6(S235/S236 | 272.97 | 2 | LOXIMVI-signal2 | G4 |
| JNK-IN-8 | WM1552C | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 228.44 | p-Rb(S807/S811) | 120.65 | p-S6(S235/S236 | 446.76 | 2 | WM1552C-signal2 | E3 | |
| Vemurafenib | WM1552C | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 216.59 | p-Rb(S807/S811) | 117.74 | p-S6(S235/S236 | 410.72 | 2 | WM1552C-signal2 | C11 | |
| Vemurafenib | WM1552C | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 221.69 | p-Rb(S807/S811) | 117.69 | p-S6(S235/S236 | 423.70 | 1 | WM1552C-signal2 | B11 | |
| JNK-IN-8 | WM1552C | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 226.81 | p-Rb(S807/S811) | 116.31 | p-S6(S235/S236 | 435.53 | 1 | WM1552C-signal2 | D3 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 215.02 | p-Rb(S807/S811) | 116.21 | p-S6(S235/S236 | 424.78 | 2 | WM1552C-signal2 | G9 |