Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | Ki-67 | 680.51 | p-Rb(S807/S811) | 40.34 | p-cJun(S73) | 71.07 | 2 | Colo858-signal3 | C4 | |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | Ki-67 | 678.24 | p-Rb(S807/S811) | 45.98 | p-cJun(S73) | 72.90 | 1 | Colo858-signal3 | B4 | |
| Vemurafenib | WM-115 | 0.0512 | uM | 24 | hr | | Ki-67 | 678.09 | p-Rb(S807/S811) | 65.24 | p-cJun(S73) | 51.35 | 2 | WM115-signal3 | C7 | |
| Vemurafenib | WM-115 | 2.0000 | uM | 24 | hr | | Ki-67 | 671.20 | p-Rb(S807/S811) | 44.63 | p-cJun(S73) | 68.10 | 1 | WM115-signal3 | B3 | |
| Vemurafenib | WM-115 | 0.8000 | uM | 24 | hr | | Ki-67 | 666.78 | p-Rb(S807/S811) | 38.55 | p-cJun(S73) | 82.82 | 2 | WM115-signal3 | C4 | |
| Vemurafenib | WM-115 | 0.8000 | uM | 24 | hr | | Ki-67 | 663.63 | p-Rb(S807/S811) | 39.63 | p-cJun(S73) | 79.94 | 1 | WM115-signal3 | B4 | |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | Ki-67 | 660.32 | p-Rb(S807/S811) | 48.67 | p-cJun(S73) | 67.95 | 2 | Colo858-signal3 | C5 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 2.0000 | uM | 24 | hr | | Ki-67 | 657.65 | p-Rb(S807/S811) | 46.72 | p-cJun(S73) | 17.87 | 2 | WM115-signal3 | G3 |
| Vemurafenib | WM-115 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | Ki-67 | 637.76 | p-Rb(S807/S811) | 61.90 | p-cJun(S73) | 19.77 | 2 | WM115-signal3 | G2 |
| Vemurafenib | WM-115 | 5.0000 | uM | 24 | hr | | Ki-67 | 635.32 | p-Rb(S807/S811) | 49.14 | p-cJun(S73) | 49.50 | 2 | WM115-signal3 | C2 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | Ki-67 | 614.46 | p-Rb(S807/S811) | 62.48 | p-cJun(S73) | 20.25 | 1 | WM115-signal3 | F2 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 609.38 | p-Rb(S807/S811) | 99.97 | p-ERK(T202/Y204) | 70.02 | 2 | LOXIMVI-signal1 | G2 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 598.80 | p-Rb(S807/S811) | 103.77 | p-ERK(T202/Y204) | 63.79 | 1 | LOXIMVI-signal1 | F2 |
| Vemurafenib | COLO 858 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 597.47 | p-Rb(S807/S811) | 132.94 | p-ERK(T202/Y204) | 291.40 | 2 | Colo858-signal1 | C7 | |
| JNK-IN-8 | COLO 858 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 596.02 | p-Rb(S807/S811) | 233.59 | p-ERK(T202/Y204) | 707.32 | 1 | Colo858-signal1 | D3 | |
| JNK-IN-8 | COLO 858 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 594.04 | p-Rb(S807/S811) | 231.49 | p-ERK(T202/Y204) | 724.41 | 2 | Colo858-signal1 | E3 | |
| Vemurafenib | WM-115 | 0.3200 | uM | 24 | hr | | Ki-67 | 587.55 | p-Rb(S807/S811) | 26.27 | p-cJun(S73) | 71.93 | 2 | WM115-signal3 | C5 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 560.57 | p-Rb(S807/S811) | 82.73 | p-ERK(T202/Y204) | 44.60 | 1 | LOXIMVI-signal1 | F3 |
| Vemurafenib | COLO 858 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 555.61 | p-Rb(S807/S811) | 125.96 | p-ERK(T202/Y204) | 272.64 | 1 | Colo858-signal1 | B7 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 546.66 | p-Rb(S807/S811) | 87.40 | p-ERK(T202/Y204) | 40.47 | 1 | LOXIMVI-signal1 | F4 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 537.76 | p-Rb(S807/S811) | 125.84 | p-ERK(T202/Y204) | 268.22 | 1 | Colo858-signal1 | F7 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 533.87 | p-Rb(S807/S811) | 85.56 | p-ERK(T202/Y204) | 37.31 | 2 | LOXIMVI-signal1 | G3 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 525.79 | p-Rb(S807/S811) | 128.17 | p-S6(S235/S236 | 40.46 | 2 | Colo858-signal2 | G2 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 511.00 | p-Rb(S807/S811) | 125.23 | p-ERK(T202/Y204) | 254.95 | 2 | Colo858-signal1 | G7 |
| JNK-IN-8 | COLO 858 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 504.81 | p-Rb(S807/S811) | 190.17 | p-ERK(T202/Y204) | 719.29 | 2 | Colo858-signal1 | E4 |