Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 1101.80 | p-Rb(S807/S811) | 48.19 | p-ERK(T202/Y204) | 9.79 | 1 | Colo858-signal1 | B2 | |
| JNK-IN-8 | WM-115 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 225.01 | p-Rb(S807/S811) | 72.59 | p-ERK(T202/Y204) | 628.25 | 2 | WM115-signal1 | E11 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 317.63 | p-Rb(S807/S811) | 191.25 | p-ERK(T202/Y204) | 128.24 | 2 | LOXIMVI-signal1 | G8 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 325.15 | p-Rb(S807/S811) | 174.20 | p-ERK(T202/Y204) | 85.62 | 2 | LOXIMVI-signal1 | G7 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 348.86 | p-Rb(S807/S811) | 169.33 | p-ERK(T202/Y204) | 67.27 | 2 | LOXIMVI-signal1 | G6 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 432.08 | p-Rb(S807/S811) | 123.48 | p-ERK(T202/Y204) | 43.03 | 2 | LOXIMVI-signal1 | G5 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 458.83 | p-Rb(S807/S811) | 152.02 | p-ERK(T202/Y204) | 459.90 | 2 | Colo858-signal1 | G8 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 482.18 | p-Rb(S807/S811) | 101.92 | p-ERK(T202/Y204) | 31.10 | 2 | LOXIMVI-signal1 | G4 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 533.87 | p-Rb(S807/S811) | 85.56 | p-ERK(T202/Y204) | 37.31 | 2 | LOXIMVI-signal1 | G3 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 609.38 | p-Rb(S807/S811) | 99.97 | p-ERK(T202/Y204) | 70.02 | 2 | LOXIMVI-signal1 | G2 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 351.99 | p-Rb(S807/S811) | 215.31 | p-ERK(T202/Y204) | 217.61 | 2 | LOXIMVI-signal1 | G11 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 511.00 | p-Rb(S807/S811) | 125.23 | p-ERK(T202/Y204) | 254.95 | 2 | Colo858-signal1 | G7 |
| JNK-IN-8 | COLO 858 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 439.43 | p-Rb(S807/S811) | 169.34 | p-ERK(T202/Y204) | 657.25 | 1 | Colo858-signal1 | D8 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 303.58 | p-Rb(S807/S811) | 202.43 | p-ERK(T202/Y204) | 184.11 | 2 | LOXIMVI-signal1 | G10 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 310.13 | p-Rb(S807/S811) | 197.48 | p-ERK(T202/Y204) | 166.06 | 1 | LOXIMVI-signal1 | F9 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 304.49 | p-Rb(S807/S811) | 193.11 | p-ERK(T202/Y204) | 131.86 | 1 | LOXIMVI-signal1 | F8 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 338.89 | p-Rb(S807/S811) | 176.50 | p-ERK(T202/Y204) | 86.35 | 1 | LOXIMVI-signal1 | F7 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 743.36 | p-Rb(S807/S811) | 68.92 | p-ERK(T202/Y204) | 51.05 | 2 | Colo858-signal1 | G6 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 356.71 | p-Rb(S807/S811) | 166.33 | p-ERK(T202/Y204) | 71.63 | 1 | LOXIMVI-signal1 | F6 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 441.68 | p-Rb(S807/S811) | 125.56 | p-ERK(T202/Y204) | 43.30 | 1 | LOXIMVI-signal1 | F5 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 546.66 | p-Rb(S807/S811) | 87.40 | p-ERK(T202/Y204) | 40.47 | 1 | LOXIMVI-signal1 | F4 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 560.57 | p-Rb(S807/S811) | 82.73 | p-ERK(T202/Y204) | 44.60 | 1 | LOXIMVI-signal1 | F3 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 983.23 | p-Rb(S807/S811) | 56.72 | p-ERK(T202/Y204) | 28.24 | 2 | Colo858-signal1 | G5 |
| JNK-IN-8 | COLO 858 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 430.28 | p-Rb(S807/S811) | 171.77 | p-ERK(T202/Y204) | 673.40 | 1 | Colo858-signal1 | D7 | |
| Vemurafenib | COLO 858 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 1027.10 | p-Rb(S807/S811) | 46.02 | p-ERK(T202/Y204) | 14.33 | 2 | Colo858-signal1 | C3 |