Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | LOXIMVI | 2.0000 | uM | 24 | hr | | p27 Kip1 | 376.92 | p-Rb(S807/S811) | 258.15 | p-ERK(T202/Y204) | 235.58 | 1 | LOXIMVI-signal1 | D3 | |
| Vemurafenib | LOXIMVI | 0.0205 | uM | 24 | hr | | p27 Kip1 | 319.35 | p-Rb(S807/S811) | 195.10 | p-ERK(T202/Y204) | 133.04 | 2 | LOXIMVI-signal1 | C8 | |
| JNK-IN-8 | LOXIMVI | 0.0205 | uM | 24 | hr | | p27 Kip1 | 303.56 | p-Rb(S807/S811) | 209.54 | p-ERK(T202/Y204) | 206.46 | 1 | LOXIMVI-signal1 | D8 | |
| Vemurafenib | LOXIMVI | 0.3200 | uM | 24 | hr | | p27 Kip1 | 384.63 | p-Rb(S807/S811) | 153.53 | p-ERK(T202/Y204) | 56.47 | 2 | LOXIMVI-signal1 | C5 | |
| JNK-IN-8 | LOXIMVI | 0.0000 | uM | 24 | hr | | p27 Kip1 | 348.21 | p-Rb(S807/S811) | 217.92 | p-ERK(T202/Y204) | 232.50 | 1 | LOXIMVI-signal1 | D11 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 307.93 | p-Rb(S807/S811) | 203.95 | p-ERK(T202/Y204) | 169.30 | 2 | LOXIMVI-signal1 | G9 |
| Vemurafenib | LOXIMVI | 2.0000 | uM | 24 | hr | | p27 Kip1 | 457.12 | p-Rb(S807/S811) | 91.96 | p-ERK(T202/Y204) | 30.10 | 2 | LOXIMVI-signal1 | C3 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 310.13 | p-Rb(S807/S811) | 197.48 | p-ERK(T202/Y204) | 166.06 | 1 | LOXIMVI-signal1 | F9 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 560.57 | p-Rb(S807/S811) | 82.73 | p-ERK(T202/Y204) | 44.60 | 1 | LOXIMVI-signal1 | F3 |
| Vemurafenib | LOXIMVI | 0.0000 | uM | 24 | hr | | p27 Kip1 | 330.81 | p-Rb(S807/S811) | 208.35 | p-ERK(T202/Y204) | 212.82 | 2 | LOXIMVI-signal1 | C11 | |
| Vemurafenib | LOXIMVI | 0.0033 | uM | 24 | hr | | p27 Kip1 | 312.49 | p-Rb(S807/S811) | 203.40 | p-ERK(T202/Y204) | 185.08 | 2 | LOXIMVI-signal1 | C10 | |
| JNK-IN-8 | LOXIMVI | 0.0512 | uM | 24 | hr | | p27 Kip1 | 303.12 | p-Rb(S807/S811) | 207.96 | p-ERK(T202/Y204) | 204.10 | 2 | LOXIMVI-signal1 | E7 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 546.66 | p-Rb(S807/S811) | 87.40 | p-ERK(T202/Y204) | 40.47 | 1 | LOXIMVI-signal1 | F4 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 598.80 | p-Rb(S807/S811) | 103.77 | p-ERK(T202/Y204) | 63.79 | 1 | LOXIMVI-signal1 | F2 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 432.08 | p-Rb(S807/S811) | 123.48 | p-ERK(T202/Y204) | 43.03 | 2 | LOXIMVI-signal1 | G5 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 482.18 | p-Rb(S807/S811) | 101.92 | p-ERK(T202/Y204) | 31.10 | 2 | LOXIMVI-signal1 | G4 |
| Vemurafenib | LOXIMVI | 0.0082 | uM | 24 | hr | | p27 Kip1 | 317.72 | p-Rb(S807/S811) | 203.25 | p-ERK(T202/Y204) | 167.85 | 2 | LOXIMVI-signal1 | C9 | |
| Vemurafenib | LOXIMVI | 0.0082 | uM | 24 | hr | | p27 Kip1 | 317.67 | p-Rb(S807/S811) | 200.59 | p-ERK(T202/Y204) | 170.43 | 1 | LOXIMVI-signal1 | B9 | |
| Vemurafenib | LOXIMVI | 0.0205 | uM | 24 | hr | | p27 Kip1 | 330.03 | p-Rb(S807/S811) | 203.83 | p-ERK(T202/Y204) | 137.92 | 1 | LOXIMVI-signal1 | B8 | |
| Vemurafenib | LOXIMVI | 0.8000 | uM | 24 | hr | | p27 Kip1 | 434.48 | p-Rb(S807/S811) | 132.48 | p-ERK(T202/Y204) | 43.96 | 2 | LOXIMVI-signal1 | C4 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 348.86 | p-Rb(S807/S811) | 169.33 | p-ERK(T202/Y204) | 67.27 | 2 | LOXIMVI-signal1 | G6 |
| Vemurafenib | LOXIMVI | 0.3200 | uM | 24 | hr | | p27 Kip1 | 395.79 | p-Rb(S807/S811) | 157.15 | p-ERK(T202/Y204) | 59.04 | 1 | LOXIMVI-signal1 | B5 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 325.15 | p-Rb(S807/S811) | 174.20 | p-ERK(T202/Y204) | 85.62 | 2 | LOXIMVI-signal1 | G7 |
| JNK-IN-8 | LOXIMVI | 2.0000 | uM | 24 | hr | | p27 Kip1 | 368.98 | p-Rb(S807/S811) | 251.60 | p-ERK(T202/Y204) | 236.95 | 2 | LOXIMVI-signal1 | E3 | |
| JNK-IN-8 | LOXIMVI | 5.0000 | uM | 24 | hr | | p27 Kip1 | 464.53 | p-Rb(S807/S811) | 268.11 | p-ERK(T202/Y204) | 267.97 | 1 | LOXIMVI-signal1 | D2 |