Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | WM-115 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 227.05 | p-Rb(S807/S811) | 74.53 | p-ERK(T202/Y204) | 625.81 | 1 | WM115-signal1 | D10 | |
| Vemurafenib | WM-115 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 230.38 | p-Rb(S807/S811) | 73.32 | p-ERK(T202/Y204) | 501.50 | 2 | WM115-signal1 | C8 | |
| JNK-IN-8 | WM-115 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 235.89 | p-Rb(S807/S811) | 78.16 | p-ERK(T202/Y204) | 655.44 | 1 | WM115-signal1 | D8 | |
| Vemurafenib | WM-115 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 238.56 | p-Rb(S807/S811) | 70.44 | p-ERK(T202/Y204) | 385.55 | 2 | WM115-signal1 | C7 | |
| Vemurafenib | WM-115 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 235.53 | p-Rb(S807/S811) | 54.90 | p-ERK(T202/Y204) | 152.31 | 2 | WM115-signal1 | C6 | |
| Vemurafenib | WM-115 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 224.35 | p-Rb(S807/S811) | 70.42 | p-ERK(T202/Y204) | 575.35 | 2 | WM115-signal1 | C9 | |
| Vemurafenib | WM-115 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 318.36 | p-Rb(S807/S811) | 43.44 | p-ERK(T202/Y204) | 16.10 | 2 | WM115-signal1 | C3 | |
| Vemurafenib | WM-115 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 333.60 | p-Rb(S807/S811) | 45.80 | p-ERK(T202/Y204) | 12.42 | 2 | WM115-signal1 | C2 | |
| Vemurafenib | WM-115 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 301.62 | p-Rb(S807/S811) | 38.59 | p-ERK(T202/Y204) | 24.54 | 2 | WM115-signal1 | C4 | |
| Vemurafenib | WM-115 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 224.32 | p-Rb(S807/S811) | 72.50 | p-ERK(T202/Y204) | 614.52 | 2 | WM115-signal1 | C10 | |
| Vemurafenib | WM-115 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 230.43 | p-Rb(S807/S811) | 73.12 | p-ERK(T202/Y204) | 557.15 | 1 | WM115-signal1 | B9 | |
| Vemurafenib | WM-115 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 238.61 | p-Rb(S807/S811) | 73.09 | p-ERK(T202/Y204) | 620.90 | 2 | WM115-signal1 | C11 | |
| Vemurafenib | WM-115 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 257.65 | p-Rb(S807/S811) | 38.40 | p-ERK(T202/Y204) | 44.96 | 2 | WM115-signal1 | C5 | |
| Vemurafenib | WM-115 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 304.70 | p-Rb(S807/S811) | 38.57 | p-ERK(T202/Y204) | 23.27 | 1 | WM115-signal1 | B4 | |
| Vemurafenib | WM-115 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 325.41 | p-Rb(S807/S811) | 43.62 | p-ERK(T202/Y204) | 15.11 | 1 | WM115-signal1 | B3 | |
| Vemurafenib | WM-115 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 263.18 | p-Rb(S807/S811) | 37.97 | p-ERK(T202/Y204) | 43.14 | 1 | WM115-signal1 | B5 | |
| Vemurafenib | WM-115 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 242.95 | p-Rb(S807/S811) | 73.13 | p-ERK(T202/Y204) | 635.44 | 1 | WM115-signal1 | B11 | |
| Vemurafenib | WM-115 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 228.00 | p-Rb(S807/S811) | 72.02 | p-ERK(T202/Y204) | 575.75 | 1 | WM115-signal1 | B10 | |
| Vemurafenib | WM-115 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 333.89 | p-Rb(S807/S811) | 50.05 | p-ERK(T202/Y204) | 12.54 | 1 | WM115-signal1 | B2 | |
| Vemurafenib | WM-115 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 237.94 | p-Rb(S807/S811) | 55.20 | p-ERK(T202/Y204) | 149.42 | 1 | WM115-signal1 | B6 | |
| Vemurafenib | WM-115 | 0.0205 | uM | 24 | hr | | Ki-67 | 780.78 | p-Rb(S807/S811) | 72.14 | p-cJun(S73) | 50.93 | 1 | WM115-signal3 | B8 | |
| Vemurafenib | WM-115 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 237.09 | p-Rb(S807/S811) | 68.88 | p-ERK(T202/Y204) | 366.99 | 1 | WM115-signal1 | B7 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | Ki-67 | 796.29 | p-Rb(S807/S811) | 74.93 | p-cJun(S73) | 42.86 | 2 | WM115-signal3 | G8 |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | Ki-67 | 764.21 | p-Rb(S807/S811) | 71.74 | p-cJun(S73) | 39.60 | 2 | WM115-signal3 | G7 |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0082 | uM | 24 | hr | | Ki-67 | 732.41 | p-Rb(S807/S811) | 74.91 | p-cJun(S73) | 46.30 | 2 | WM115-signal3 | G9 |