Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 223.67 | p-Rb(S807/S811) | 74.26 | p-ERK(T202/Y204) | 488.45 | 2 | WM115-signal1 | G8 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 245.42 | p-Rb(S807/S811) | 98.27 | p-ERK(T202/Y204) | 209.11 | 2 | WM1552C-signal1 | G8 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 197.88 | p-Rb(S807/S811) | 110.31 | p-S6(S235/S236 | 410.75 | 2 | WM1552C-signal2 | G8 |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | Ki-67 | 796.29 | p-Rb(S807/S811) | 74.93 | p-cJun(S73) | 42.86 | 2 | WM115-signal3 | G8 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0205 | uM | 24 | hr | | Ki-67 | 1542.10 | p-Rb(S807/S811) | 107.52 | p-cJun(S73) | 159.51 | 2 | WM1552C-signal3 | G8 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | Ki-67 | 2064.40 | p-Rb(S807/S811) | 152.34 | p-cJun(S73) | 26.96 | 2 | Colo858-signal3 | G8 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0205 | uM | 24 | hr | | Ki-67 | 2617.50 | p-Rb(S807/S811) | 180.39 | p-cJun(S73) | 69.18 | 2 | LOXIMVI-signal3 | G8 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 327.85 | p-Rb(S807/S811) | 162.48 | p-S6(S235/S236 | 410.06 | 2 | Colo858-signal2 | G8 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 456.27 | p-Rb(S807/S811) | 159.28 | p-ERK(T202/Y204) | 556.01 | 2 | Colo858-signal1 | G9 |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 231.77 | p-Rb(S807/S811) | 74.86 | p-ERK(T202/Y204) | 564.70 | 2 | WM115-signal1 | G9 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 260.82 | p-Rb(S807/S811) | 220.20 | p-S6(S235/S236 | 459.82 | 2 | LOXIMVI-signal2 | G9 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 307.93 | p-Rb(S807/S811) | 203.95 | p-ERK(T202/Y204) | 169.30 | 2 | LOXIMVI-signal1 | G9 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0082 | uM | 24 | hr | | Ki-67 | 1581.10 | p-Rb(S807/S811) | 104.30 | p-cJun(S73) | 145.59 | 2 | WM1552C-signal3 | G9 |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 181.02 | p-Rb(S807/S811) | 67.72 | p-S6(S235/S236 | 155.98 | 2 | WM115-signal2 | G9 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 343.94 | p-Rb(S807/S811) | 170.97 | p-S6(S235/S236 | 450.82 | 2 | Colo858-signal2 | G9 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 215.02 | p-Rb(S807/S811) | 116.21 | p-S6(S235/S236 | 424.78 | 2 | WM1552C-signal2 | G9 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0082 | uM | 24 | hr | | Ki-67 | 2667.60 | p-Rb(S807/S811) | 189.57 | p-cJun(S73) | 84.40 | 2 | LOXIMVI-signal3 | G9 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 241.65 | p-Rb(S807/S811) | 104.81 | p-ERK(T202/Y204) | 245.66 | 2 | WM1552C-signal1 | G9 |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0082 | uM | 24 | hr | | Ki-67 | 732.41 | p-Rb(S807/S811) | 74.91 | p-cJun(S73) | 46.30 | 2 | WM115-signal3 | G9 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0082 | uM | 24 | hr | | Ki-67 | 2282.60 | p-Rb(S807/S811) | 152.28 | p-cJun(S73) | 28.92 | 2 | Colo858-signal3 | G9 |