Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | WM1552C | 5.0000 | uM | 24 | hr | | Ki-67 | 973.61 | p-Rb(S807/S811) | 47.01 | p-cJun(S73) | 59.51 | 1 | WM1552C-signal3 | B2 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.8000 | uM | 24 | hr | | Ki-67 | 747.98 | p-Rb(S807/S811) | 47.12 | p-cJun(S73) | 13.64 | 2 | Colo858-signal3 | G4 |
| Vemurafenib | WM1552C | JNK-IN-8 | 2.0000 | uM | 24 | hr | | Ki-67 | 1000.20 | p-Rb(S807/S811) | 47.24 | p-cJun(S73) | 12.90 | 1 | WM1552C-signal3 | F3 |
| Vemurafenib | WM-115 | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 261.27 | p-Rb(S807/S811) | 47.45 | p-S6(S235/S236 | 41.16 | 1 | WM115-signal2 | F3 |
| Vemurafenib | WM-115 | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 339.45 | p-Rb(S807/S811) | 47.47 | p-ERK(T202/Y204) | 20.55 | 2 | WM115-signal1 | G3 |
| Vemurafenib | WM-115 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 210.36 | p-Rb(S807/S811) | 47.67 | p-S6(S235/S236 | 54.23 | 2 | WM115-signal2 | C3 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 1176.20 | p-Rb(S807/S811) | 47.97 | p-ERK(T202/Y204) | 25.00 | 1 | Colo858-signal1 | F4 |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 1014.60 | p-Rb(S807/S811) | 48.01 | p-ERK(T202/Y204) | 18.67 | 2 | Colo858-signal1 | C4 | |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | Ki-67 | 708.08 | p-Rb(S807/S811) | 48.09 | p-cJun(S73) | 72.08 | 1 | Colo858-signal3 | B5 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 478.70 | p-Rb(S807/S811) | 48.18 | p-ERK(T202/Y204) | 22.45 | 1 | WM1552C-signal1 | F3 |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 1101.80 | p-Rb(S807/S811) | 48.19 | p-ERK(T202/Y204) | 9.79 | 1 | Colo858-signal1 | B2 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 1044.90 | p-Rb(S807/S811) | 48.21 | p-ERK(T202/Y204) | 22.38 | 2 | Colo858-signal1 | G4 |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 1063.20 | p-Rb(S807/S811) | 48.29 | p-ERK(T202/Y204) | 17.48 | 1 | Colo858-signal1 | B4 | |
| Vemurafenib | WM-115 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 209.18 | p-Rb(S807/S811) | 48.45 | p-S6(S235/S236 | 30.86 | 2 | WM115-signal2 | C2 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.8000 | uM | 24 | hr | | Ki-67 | 807.26 | p-Rb(S807/S811) | 48.49 | p-cJun(S73) | 14.54 | 1 | Colo858-signal3 | F4 |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | Ki-67 | 660.32 | p-Rb(S807/S811) | 48.67 | p-cJun(S73) | 67.95 | 2 | Colo858-signal3 | C5 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 1024.70 | p-Rb(S807/S811) | 48.87 | p-ERK(T202/Y204) | 30.22 | 1 | Colo858-signal1 | F5 |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 1028.10 | p-Rb(S807/S811) | 48.93 | p-ERK(T202/Y204) | 27.49 | 2 | Colo858-signal1 | C5 | |
| Vemurafenib | WM1552C | 0.8000 | uM | 24 | hr | | p27 Kip1 | 440.30 | p-Rb(S807/S811) | 48.95 | p-ERK(T202/Y204) | 28.75 | 2 | WM1552C-signal1 | C4 | |
| Vemurafenib | WM1552C | 5.0000 | uM | 24 | hr | | Ki-67 | 955.89 | p-Rb(S807/S811) | 49.08 | p-cJun(S73) | 59.90 | 2 | WM1552C-signal3 | C2 | |
| Vemurafenib | WM-115 | 5.0000 | uM | 24 | hr | | Ki-67 | 635.32 | p-Rb(S807/S811) | 49.14 | p-cJun(S73) | 49.50 | 2 | WM115-signal3 | C2 | |
| Vemurafenib | WM-115 | 5.0000 | uM | 24 | hr | | Ki-67 | 691.81 | p-Rb(S807/S811) | 49.35 | p-cJun(S73) | 49.25 | 1 | WM115-signal3 | B2 | |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 1100.60 | p-Rb(S807/S811) | 49.89 | p-ERK(T202/Y204) | 9.41 | 2 | Colo858-signal1 | C2 | |
| Vemurafenib | WM-115 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 333.89 | p-Rb(S807/S811) | 50.05 | p-ERK(T202/Y204) | 12.54 | 1 | WM115-signal1 | B2 | |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 964.47 | p-Rb(S807/S811) | 50.08 | p-ERK(T202/Y204) | 25.09 | 1 | Colo858-signal1 | B5 |