Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | LOXIMVI | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 291.53 | p-Rb(S807/S811) | 246.99 | p-S6(S235/S236 | 502.86 | 1 | LOXIMVI-signal2 | D5 | |
| JNK-IN-8 | LOXIMVI | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 344.33 | p-Rb(S807/S811) | 248.42 | p-S6(S235/S236 | 524.11 | 1 | LOXIMVI-signal2 | D4 | |
| JNK-IN-8 | COLO 858 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 738.89 | p-Rb(S807/S811) | 249.29 | p-ERK(T202/Y204) | 766.04 | 1 | Colo858-signal1 | D2 | |
| JNK-IN-8 | LOXIMVI | 2.0000 | uM | 24 | hr | | Ki-67 | 3091.80 | p-Rb(S807/S811) | 251.41 | p-cJun(S73) | 53.20 | 2 | LOXIMVI-signal3 | E3 | |
| JNK-IN-8 | LOXIMVI | 2.0000 | uM | 24 | hr | | p27 Kip1 | 368.98 | p-Rb(S807/S811) | 251.60 | p-ERK(T202/Y204) | 236.95 | 2 | LOXIMVI-signal1 | E3 | |
| JNK-IN-8 | COLO 858 | 5.0000 | uM | 24 | hr | | Ki-67 | 2634.30 | p-Rb(S807/S811) | 252.91 | p-cJun(S73) | 39.50 | 2 | Colo858-signal3 | E2 | |
| JNK-IN-8 | COLO 858 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 315.08 | p-Rb(S807/S811) | 253.54 | p-S6(S235/S236 | 620.64 | 1 | Colo858-signal2 | D2 | |
| JNK-IN-8 | COLO 858 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 839.15 | p-Rb(S807/S811) | 256.43 | p-ERK(T202/Y204) | 789.95 | 2 | Colo858-signal1 | E2 | |
| JNK-IN-8 | LOXIMVI | 2.0000 | uM | 24 | hr | | Ki-67 | 3123.80 | p-Rb(S807/S811) | 257.55 | p-cJun(S73) | 54.72 | 1 | LOXIMVI-signal3 | D3 | |
| JNK-IN-8 | LOXIMVI | 2.0000 | uM | 24 | hr | | p27 Kip1 | 376.92 | p-Rb(S807/S811) | 258.15 | p-ERK(T202/Y204) | 235.58 | 1 | LOXIMVI-signal1 | D3 | |
| JNK-IN-8 | LOXIMVI | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 297.60 | p-Rb(S807/S811) | 258.35 | p-S6(S235/S236 | 550.62 | 2 | LOXIMVI-signal2 | E4 | |
| JNK-IN-8 | COLO 858 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 370.03 | p-Rb(S807/S811) | 260.57 | p-S6(S235/S236 | 614.60 | 2 | Colo858-signal2 | E2 | |
| JNK-IN-8 | LOXIMVI | 5.0000 | uM | 24 | hr | | p27 Kip1 | 464.53 | p-Rb(S807/S811) | 268.11 | p-ERK(T202/Y204) | 267.97 | 1 | LOXIMVI-signal1 | D2 | |
| JNK-IN-8 | LOXIMVI | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 368.60 | p-Rb(S807/S811) | 272.58 | p-S6(S235/S236 | 627.74 | 1 | LOXIMVI-signal2 | D3 | |
| JNK-IN-8 | LOXIMVI | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 377.73 | p-Rb(S807/S811) | 274.50 | p-S6(S235/S236 | 619.52 | 2 | LOXIMVI-signal2 | E3 | |
| JNK-IN-8 | LOXIMVI | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 388.24 | p-Rb(S807/S811) | 277.19 | p-S6(S235/S236 | 582.84 | 1 | LOXIMVI-signal2 | D2 | |
| JNK-IN-8 | LOXIMVI | 5.0000 | uM | 24 | hr | | p27 Kip1 | 472.18 | p-Rb(S807/S811) | 278.69 | p-ERK(T202/Y204) | 272.54 | 2 | LOXIMVI-signal1 | E2 | |
| JNK-IN-8 | LOXIMVI | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 394.33 | p-Rb(S807/S811) | 290.15 | p-S6(S235/S236 | 433.67 | 2 | LOXIMVI-signal2 | E2 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | p-Rb(S807/S811) | p-S6(S235/S236 | 1 | WM115-signal2 | F8 | |||
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | p-Rb(S807/S811) | p-S6(S235/S236 | 1 | WM115-signal2 | F9 |