Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | COLO 858 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 438.30 | p-Rb(S807/S811) | 160.10 | p-ERK(T202/Y204) | 626.02 | 1 | Colo858-signal1 | D10 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 459.60 | p-Rb(S807/S811) | 160.13 | p-ERK(T202/Y204) | 573.25 | 1 | Colo858-signal1 | F10 |
| JNK-IN-8 | COLO 858 | 0.0512 | uM | 24 | hr | | Ki-67 | 2172.00 | p-Rb(S807/S811) | 160.38 | p-cJun(S73) | 29.06 | 2 | Colo858-signal3 | E7 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 311.80 | p-Rb(S807/S811) | 160.43 | p-S6(S235/S236 | 378.06 | 1 | Colo858-signal2 | F9 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 461.45 | p-Rb(S807/S811) | 160.46 | p-ERK(T202/Y204) | 599.65 | 1 | Colo858-signal1 | F9 |
| Vemurafenib | COLO 858 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 489.20 | p-Rb(S807/S811) | 160.66 | p-ERK(T202/Y204) | 628.14 | 2 | Colo858-signal1 | C10 | |
| JNK-IN-8 | COLO 858 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 321.22 | p-Rb(S807/S811) | 160.77 | p-S6(S235/S236 | 385.74 | 2 | Colo858-signal2 | E10 | |
| JNK-IN-8 | COLO 858 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 442.59 | p-Rb(S807/S811) | 160.97 | p-ERK(T202/Y204) | 615.32 | 2 | Colo858-signal1 | E10 | |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 377.25 | p-Rb(S807/S811) | 161.02 | p-S6(S235/S236 | 380.19 | 2 | Colo858-signal2 | C11 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 447.88 | p-Rb(S807/S811) | 161.30 | p-ERK(T202/Y204) | 568.39 | 2 | Colo858-signal1 | G11 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 336.86 | p-Rb(S807/S811) | 161.69 | p-S6(S235/S236 | 384.38 | 1 | Colo858-signal2 | F11 |
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 307.92 | p-Rb(S807/S811) | 162.01 | p-S6(S235/S236 | 403.15 | 1 | Colo858-signal2 | B8 | |
| Vemurafenib | COLO 858 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 326.58 | p-Rb(S807/S811) | 162.30 | p-S6(S235/S236 | 402.84 | 1 | Colo858-signal2 | B10 | |
| JNK-IN-8 | COLO 858 | 0.1280 | uM | 24 | hr | | Ki-67 | 2233.20 | p-Rb(S807/S811) | 162.30 | p-cJun(S73) | 29.52 | 2 | Colo858-signal3 | E6 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 327.85 | p-Rb(S807/S811) | 162.48 | p-S6(S235/S236 | 410.06 | 2 | Colo858-signal2 | G8 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 300.67 | p-Rb(S807/S811) | 162.97 | p-S6(S235/S236 | 433.52 | 2 | Colo858-signal2 | G10 |
| Vemurafenib | COLO 858 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 461.92 | p-Rb(S807/S811) | 163.29 | p-ERK(T202/Y204) | 613.35 | 2 | Colo858-signal1 | C9 | |
| JNK-IN-8 | COLO 858 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 373.77 | p-Rb(S807/S811) | 163.58 | p-S6(S235/S236 | 386.62 | 2 | Colo858-signal2 | E9 | |
| Vemurafenib | COLO 858 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 254.30 | p-Rb(S807/S811) | 163.98 | p-S6(S235/S236 | 398.31 | 2 | Colo858-signal2 | C10 | |
| Vemurafenib | COLO 858 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 301.75 | p-Rb(S807/S811) | 164.37 | p-S6(S235/S236 | 394.95 | 2 | Colo858-signal2 | C9 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 376.71 | p-Rb(S807/S811) | 165.48 | p-S6(S235/S236 | 397.13 | 2 | Colo858-signal2 | G11 |
| JNK-IN-8 | COLO 858 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 323.83 | p-Rb(S807/S811) | 165.88 | p-S6(S235/S236 | 385.36 | 1 | Colo858-signal2 | D11 | |
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 349.40 | p-Rb(S807/S811) | 166.03 | p-S6(S235/S236 | 385.58 | 2 | Colo858-signal2 | C8 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 356.71 | p-Rb(S807/S811) | 166.33 | p-ERK(T202/Y204) | 71.63 | 1 | LOXIMVI-signal1 | F6 |
| JNK-IN-8 | COLO 858 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 311.93 | p-Rb(S807/S811) | 166.54 | p-S6(S235/S236 | 387.41 | 2 | Colo858-signal2 | E8 |