Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | COLO 858 | 0.0512 | uM | 24 | hr | | Ki-67 | 2200.30 | p-Rb(S807/S811) | 153.90 | p-cJun(S73) | 30.11 | 1 | Colo858-signal3 | D7 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 462.46 | p-Rb(S807/S811) | 154.40 | p-ERK(T202/Y204) | 499.08 | 1 | Colo858-signal1 | F8 |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | Ki-67 | 2259.80 | p-Rb(S807/S811) | 154.64 | p-cJun(S73) | 29.83 | 2 | Colo858-signal3 | C11 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 421.06 | p-Rb(S807/S811) | 154.67 | p-ERK(T202/Y204) | 537.34 | 2 | Colo858-signal1 | G10 |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 452.92 | p-Rb(S807/S811) | 154.74 | p-ERK(T202/Y204) | 648.89 | 2 | Colo858-signal1 | C11 | |
| Vemurafenib | LOXIMVI | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 239.52 | p-Rb(S807/S811) | 154.83 | p-S6(S235/S236 | 375.76 | 1 | LOXIMVI-signal2 | B5 | |
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 483.48 | p-Rb(S807/S811) | 154.86 | p-ERK(T202/Y204) | 515.79 | 2 | Colo858-signal1 | C8 | |
| JNK-IN-8 | COLO 858 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 436.36 | p-Rb(S807/S811) | 155.67 | p-ERK(T202/Y204) | 648.15 | 1 | Colo858-signal1 | D9 | |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 358.86 | p-Rb(S807/S811) | 155.99 | p-S6(S235/S236 | 377.96 | 1 | Colo858-signal2 | B11 | |
| JNK-IN-8 | COLO 858 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 438.93 | p-Rb(S807/S811) | 156.13 | p-ERK(T202/Y204) | 620.57 | 2 | Colo858-signal1 | E9 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 337.75 | p-Rb(S807/S811) | 156.22 | p-S6(S235/S236 | 357.37 | 1 | Colo858-signal2 | F8 |
| JNK-IN-8 | COLO 858 | 0.0205 | uM | 24 | hr | | Ki-67 | 2267.30 | p-Rb(S807/S811) | 156.38 | p-cJun(S73) | 31.07 | 1 | Colo858-signal3 | D8 | |
| JNK-IN-8 | COLO 858 | 0.0205 | uM | 24 | hr | | Ki-67 | 2269.90 | p-Rb(S807/S811) | 156.97 | p-cJun(S73) | 29.44 | 2 | Colo858-signal3 | E8 | |
| Vemurafenib | LOXIMVI | 0.3200 | uM | 24 | hr | | p27 Kip1 | 395.79 | p-Rb(S807/S811) | 157.15 | p-ERK(T202/Y204) | 59.04 | 1 | LOXIMVI-signal1 | B5 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 453.65 | p-Rb(S807/S811) | 157.18 | p-ERK(T202/Y204) | 608.40 | 1 | Colo858-signal1 | F11 |
| Vemurafenib | LOXIMVI | 0.1280 | uM | 24 | hr | | Ki-67 | 2570.00 | p-Rb(S807/S811) | 157.19 | p-cJun(S73) | 85.99 | 2 | LOXIMVI-signal3 | C6 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.1280 | uM | 24 | hr | | Ki-67 | 2553.90 | p-Rb(S807/S811) | 157.20 | p-cJun(S73) | 41.82 | 1 | LOXIMVI-signal3 | F6 |
| JNK-IN-8 | COLO 858 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 445.15 | p-Rb(S807/S811) | 158.36 | p-ERK(T202/Y204) | 601.12 | 2 | Colo858-signal1 | E11 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0033 | uM | 24 | hr | | Ki-67 | 2368.00 | p-Rb(S807/S811) | 158.88 | p-cJun(S73) | 29.06 | 2 | Colo858-signal3 | G10 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0000 | uM | 24 | hr | | Ki-67 | 2392.50 | p-Rb(S807/S811) | 158.97 | p-cJun(S73) | 29.83 | 2 | Colo858-signal3 | G11 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 456.27 | p-Rb(S807/S811) | 159.28 | p-ERK(T202/Y204) | 556.01 | 2 | Colo858-signal1 | G9 |
| JNK-IN-8 | COLO 858 | 0.1280 | uM | 24 | hr | | Ki-67 | 2238.80 | p-Rb(S807/S811) | 159.49 | p-cJun(S73) | 30.74 | 1 | Colo858-signal3 | D6 | |
| Vemurafenib | COLO 858 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 452.40 | p-Rb(S807/S811) | 159.51 | p-ERK(T202/Y204) | 579.26 | 1 | Colo858-signal1 | B10 | |
| JNK-IN-8 | COLO 858 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 454.18 | p-Rb(S807/S811) | 159.65 | p-ERK(T202/Y204) | 628.56 | 1 | Colo858-signal1 | D11 | |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 479.59 | p-Rb(S807/S811) | 159.95 | p-ERK(T202/Y204) | 654.45 | 1 | Colo858-signal1 | B11 |