Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
Download: Excel (XLSX), Comma-separated (CSV)
| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | WM1552C | 2.0000 | uM | 24 | hr | | p27 Kip1 | 472.02 | p-Rb(S807/S811) | 42.22 | p-ERK(T202/Y204) | 19.71 | 2 | WM1552C-signal1 | C3 | |
| Vemurafenib | WM-115 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 227.79 | p-Rb(S807/S811) | 42.29 | p-S6(S235/S236 | 56.17 | 1 | WM115-signal2 | B3 | |
| Vemurafenib | WM1552C | 2.0000 | uM | 24 | hr | | p27 Kip1 | 473.39 | p-Rb(S807/S811) | 42.61 | p-ERK(T202/Y204) | 22.62 | 1 | WM1552C-signal1 | B3 | |
| Vemurafenib | COLO 858 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 1016.50 | p-Rb(S807/S811) | 42.64 | p-ERK(T202/Y204) | 14.04 | 1 | Colo858-signal1 | B3 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 277.38 | p-Rb(S807/S811) | 42.72 | p-ERK(T202/Y204) | 54.07 | 2 | WM115-signal1 | G5 |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.8000 | uM | 24 | hr | | Ki-67 | 744.48 | p-Rb(S807/S811) | 42.82 | p-cJun(S73) | 20.07 | 1 | WM115-signal3 | F4 |
| Vemurafenib | WM-115 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 318.36 | p-Rb(S807/S811) | 43.44 | p-ERK(T202/Y204) | 16.10 | 2 | WM115-signal1 | C3 | |
| Vemurafenib | WM-115 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 325.41 | p-Rb(S807/S811) | 43.62 | p-ERK(T202/Y204) | 15.11 | 1 | WM115-signal1 | B3 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 315.18 | p-Rb(S807/S811) | 43.87 | p-ERK(T202/Y204) | 29.26 | 1 | WM115-signal1 | F4 |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | Ki-67 | 801.46 | p-Rb(S807/S811) | 44.01 | p-cJun(S73) | 45.24 | 1 | Colo858-signal3 | B2 | |
| Vemurafenib | WM-115 | 2.0000 | uM | 24 | hr | | Ki-67 | 688.19 | p-Rb(S807/S811) | 44.09 | p-cJun(S73) | 69.41 | 2 | WM115-signal3 | C3 | |
| Vemurafenib | WM1552C | 2.0000 | uM | 24 | hr | | Ki-67 | 885.89 | p-Rb(S807/S811) | 44.19 | p-cJun(S73) | 99.31 | 2 | WM1552C-signal3 | C3 | |
| Vemurafenib | WM-115 | 2.0000 | uM | 24 | hr | | Ki-67 | 671.20 | p-Rb(S807/S811) | 44.63 | p-cJun(S73) | 68.10 | 1 | WM115-signal3 | B3 | |
| Vemurafenib | COLO 858 | 2.0000 | uM | 24 | hr | | Ki-67 | 772.11 | p-Rb(S807/S811) | 44.73 | p-cJun(S73) | 66.84 | 1 | Colo858-signal3 | B3 | |
| Vemurafenib | WM1552C | 5.0000 | uM | 24 | hr | | p27 Kip1 | 446.61 | p-Rb(S807/S811) | 44.76 | p-ERK(T202/Y204) | 19.95 | 1 | WM1552C-signal1 | B2 | |
| Vemurafenib | COLO 858 | 2.0000 | uM | 24 | hr | | Ki-67 | 782.90 | p-Rb(S807/S811) | 45.43 | p-cJun(S73) | 59.46 | 2 | Colo858-signal3 | C3 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 241.43 | p-Rb(S807/S811) | 45.71 | p-S6(S235/S236 | 39.27 | 2 | WM115-signal2 | G3 |
| Vemurafenib | WM-115 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 231.89 | p-Rb(S807/S811) | 45.77 | p-S6(S235/S236 | 35.99 | 1 | WM115-signal2 | B2 | |
| Vemurafenib | WM-115 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 333.60 | p-Rb(S807/S811) | 45.80 | p-ERK(T202/Y204) | 12.42 | 2 | WM115-signal1 | C2 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 337.11 | p-Rb(S807/S811) | 45.84 | p-ERK(T202/Y204) | 20.19 | 1 | WM115-signal1 | F3 |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | Ki-67 | 678.24 | p-Rb(S807/S811) | 45.98 | p-cJun(S73) | 72.90 | 1 | Colo858-signal3 | B4 | |
| Vemurafenib | COLO 858 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 1027.10 | p-Rb(S807/S811) | 46.02 | p-ERK(T202/Y204) | 14.33 | 2 | Colo858-signal1 | C3 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 479.59 | p-Rb(S807/S811) | 46.48 | p-ERK(T202/Y204) | 22.78 | 2 | WM1552C-signal1 | G3 |
| Vemurafenib | WM-115 | JNK-IN-8 | 2.0000 | uM | 24 | hr | | Ki-67 | 657.65 | p-Rb(S807/S811) | 46.72 | p-cJun(S73) | 17.87 | 2 | WM115-signal3 | G3 |
| Vemurafenib | WM1552C | JNK-IN-8 | 2.0000 | uM | 24 | hr | | Ki-67 | 974.64 | p-Rb(S807/S811) | 46.90 | p-cJun(S73) | 13.63 | 2 | WM1552C-signal3 | G3 |