Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | LOXIMVI | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 184.72 | p-Rb(S807/S811) | 84.06 | p-S6(S235/S236 | 180.02 | 2 | LOXIMVI-signal2 | C3 | |
| JNK-IN-8 | WM-115 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 232.47 | p-Rb(S807/S811) | 84.23 | p-ERK(T202/Y204) | 633.23 | 2 | WM115-signal1 | E5 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.1280 | uM | 24 | hr | | Ki-67 | 1090.60 | p-Rb(S807/S811) | 84.26 | p-cJun(S73) | 33.13 | 1 | Colo858-signal3 | F6 |
| JNK-IN-8 | WM-115 | 2.0000 | uM | 24 | hr | | Ki-67 | 815.32 | p-Rb(S807/S811) | 84.33 | p-cJun(S73) | 22.27 | 2 | WM115-signal3 | E3 | |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | p27 Kip1 | 487.55 | p-Rb(S807/S811) | 84.67 | p-ERK(T202/Y204) | 31.63 | 2 | LOXIMVI-signal1 | C2 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 194.38 | p-Rb(S807/S811) | 84.75 | p-S6(S235/S236 | 196.38 | 2 | Colo858-signal2 | G6 |
| JNK-IN-8 | WM-115 | 0.8000 | uM | 24 | hr | | Ki-67 | 800.51 | p-Rb(S807/S811) | 84.77 | p-cJun(S73) | 21.81 | 2 | WM115-signal3 | E4 | |
| JNK-IN-8 | WM-115 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 250.36 | p-Rb(S807/S811) | 85.23 | p-ERK(T202/Y204) | 658.54 | 1 | WM115-signal1 | D6 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.1280 | uM | 24 | hr | | Ki-67 | 1366.10 | p-Rb(S807/S811) | 85.28 | p-cJun(S73) | 140.57 | 2 | WM1552C-signal3 | G6 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 533.87 | p-Rb(S807/S811) | 85.56 | p-ERK(T202/Y204) | 37.31 | 2 | LOXIMVI-signal1 | G3 |
| JNK-IN-8 | WM-115 | 5.0000 | uM | 24 | hr | | Ki-67 | 867.38 | p-Rb(S807/S811) | 85.79 | p-cJun(S73) | 23.05 | 1 | WM115-signal3 | D2 | |
| Vemurafenib | WM1552C | 0.1280 | uM | 24 | hr | | Ki-67 | 1348.40 | p-Rb(S807/S811) | 86.19 | p-cJun(S73) | 162.31 | 2 | WM1552C-signal3 | C6 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 546.66 | p-Rb(S807/S811) | 87.40 | p-ERK(T202/Y204) | 40.47 | 1 | LOXIMVI-signal1 | F4 |
| JNK-IN-8 | WM-115 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 254.69 | p-Rb(S807/S811) | 87.41 | p-ERK(T202/Y204) | 724.06 | 1 | WM115-signal1 | D3 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 279.48 | p-Rb(S807/S811) | 87.64 | p-ERK(T202/Y204) | 117.21 | 1 | WM1552C-signal1 | F6 |
| Vemurafenib | WM1552C | 0.1280 | uM | 24 | hr | | Ki-67 | 1391.60 | p-Rb(S807/S811) | 87.70 | p-cJun(S73) | 154.40 | 1 | WM1552C-signal3 | B6 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.1280 | uM | 24 | hr | | Ki-67 | 1358.60 | p-Rb(S807/S811) | 88.45 | p-cJun(S73) | 136.95 | 1 | WM1552C-signal3 | F6 |
| JNK-IN-8 | WM-115 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 250.02 | p-Rb(S807/S811) | 88.53 | p-ERK(T202/Y204) | 714.03 | 2 | WM115-signal1 | E3 | |
| Vemurafenib | WM1552C | 0.1280 | uM | 24 | hr | | p27 Kip1 | 271.43 | p-Rb(S807/S811) | 88.61 | p-ERK(T202/Y204) | 136.09 | 2 | WM1552C-signal1 | C6 | |
| Vemurafenib | WM1552C | 0.1280 | uM | 24 | hr | | p27 Kip1 | 284.96 | p-Rb(S807/S811) | 88.69 | p-ERK(T202/Y204) | 119.29 | 1 | WM1552C-signal1 | B6 | |
| JNK-IN-8 | WM-115 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 235.37 | p-Rb(S807/S811) | 88.77 | p-ERK(T202/Y204) | 657.24 | 2 | WM115-signal1 | E4 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 284.31 | p-Rb(S807/S811) | 88.80 | p-ERK(T202/Y204) | 123.78 | 2 | WM1552C-signal1 | G6 |
| JNK-IN-8 | WM-115 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 243.86 | p-Rb(S807/S811) | 88.86 | p-ERK(T202/Y204) | 663.72 | 1 | WM115-signal1 | D4 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 268.49 | p-Rb(S807/S811) | 89.43 | p-ERK(T202/Y204) | 154.75 | 1 | WM1552C-signal1 | F7 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 2.0000 | uM | 24 | hr | | Ki-67 | 2231.10 | p-Rb(S807/S811) | 90.56 | p-cJun(S73) | 23.75 | 2 | LOXIMVI-signal3 | G3 |