Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | WM-115 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 228.02 | p-Rb(S807/S811) | 77.98 | p-ERK(T202/Y204) | 657.81 | 2 | WM115-signal1 | E8 | |
| JNK-IN-8 | WM-115 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 232.27 | p-Rb(S807/S811) | 78.02 | p-S6(S235/S236 | 185.29 | 2 | WM115-signal2 | E4 | |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | Ki-67 | 2047.60 | p-Rb(S807/S811) | 78.14 | p-cJun(S73) | 80.02 | 1 | LOXIMVI-signal3 | B2 | |
| JNK-IN-8 | WM-115 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 235.89 | p-Rb(S807/S811) | 78.16 | p-ERK(T202/Y204) | 655.44 | 1 | WM115-signal1 | D8 | |
| JNK-IN-8 | WM-115 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 235.96 | p-Rb(S807/S811) | 79.36 | p-S6(S235/S236 | 186.75 | 1 | WM115-signal2 | D4 | |
| Vemurafenib | COLO 858 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 849.44 | p-Rb(S807/S811) | 79.57 | p-ERK(T202/Y204) | 75.64 | 1 | Colo858-signal1 | B6 | |
| JNK-IN-8 | WM-115 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 257.79 | p-Rb(S807/S811) | 79.63 | p-S6(S235/S236 | 207.22 | 2 | WM115-signal2 | E3 | |
| JNK-IN-8 | WM-115 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 267.33 | p-Rb(S807/S811) | 79.84 | p-ERK(T202/Y204) | 786.46 | 2 | WM115-signal1 | E2 | |
| JNK-IN-8 | WM-115 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 238.95 | p-Rb(S807/S811) | 79.96 | p-S6(S235/S236 | 176.83 | 1 | WM115-signal2 | D6 | |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 210.99 | p-Rb(S807/S811) | 80.53 | p-S6(S235/S236 | 102.92 | 1 | LOXIMVI-signal2 | B2 | |
| JNK-IN-8 | WM-115 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 238.07 | p-Rb(S807/S811) | 80.65 | p-S6(S235/S236 | 200.44 | 1 | WM115-signal2 | D3 | |
| JNK-IN-8 | WM-115 | 0.0512 | uM | 24 | hr | | Ki-67 | 765.62 | p-Rb(S807/S811) | 80.82 | p-cJun(S73) | 36.31 | 2 | WM115-signal3 | E7 | |
| JNK-IN-8 | WM-115 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 235.79 | p-Rb(S807/S811) | 81.08 | p-ERK(T202/Y204) | 651.65 | 2 | WM115-signal1 | E6 | |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | p27 Kip1 | 499.44 | p-Rb(S807/S811) | 81.11 | p-ERK(T202/Y204) | 31.87 | 1 | LOXIMVI-signal1 | B2 | |
| JNK-IN-8 | WM-115 | 0.3200 | uM | 24 | hr | | Ki-67 | 814.81 | p-Rb(S807/S811) | 81.21 | p-cJun(S73) | 22.95 | 1 | WM115-signal3 | D5 | |
| JNK-IN-8 | WM-115 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 237.86 | p-Rb(S807/S811) | 81.40 | p-ERK(T202/Y204) | 668.60 | 2 | WM115-signal1 | E7 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 738.58 | p-Rb(S807/S811) | 81.58 | p-ERK(T202/Y204) | 80.67 | 1 | Colo858-signal1 | F6 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 191.90 | p-Rb(S807/S811) | 81.85 | p-S6(S235/S236 | 199.11 | 1 | Colo858-signal2 | F6 |
| JNK-IN-8 | WM-115 | 0.8000 | uM | 24 | hr | | Ki-67 | 812.54 | p-Rb(S807/S811) | 82.01 | p-cJun(S73) | 21.32 | 1 | WM115-signal3 | D4 | |
| Vemurafenib | COLO 858 | 0.1280 | uM | 24 | hr | | Ki-67 | 1050.90 | p-Rb(S807/S811) | 82.58 | p-cJun(S73) | 62.66 | 2 | Colo858-signal3 | C6 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 560.57 | p-Rb(S807/S811) | 82.73 | p-ERK(T202/Y204) | 44.60 | 1 | LOXIMVI-signal1 | F3 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 214.37 | p-Rb(S807/S811) | 82.91 | p-S6(S235/S236 | 372.87 | 1 | WM1552C-signal2 | F9 |
| JNK-IN-8 | WM-115 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 242.63 | p-Rb(S807/S811) | 82.99 | p-ERK(T202/Y204) | 649.81 | 1 | WM115-signal1 | D7 | |
| JNK-IN-8 | WM-115 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 236.84 | p-Rb(S807/S811) | 83.75 | p-ERK(T202/Y204) | 640.13 | 1 | WM115-signal1 | D5 | |
| Vemurafenib | COLO 858 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 204.59 | p-Rb(S807/S811) | 83.78 | p-S6(S235/S236 | 220.01 | 1 | Colo858-signal2 | B6 |