Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
Download: Excel (XLSX), Comma-separated (CSV)
| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | LOXIMVI | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 290.41 | p-Rb(S807/S811) | 222.56 | p-S6(S235/S236 | 435.26 | 1 | LOXIMVI-signal2 | B10 | |
| JNK-IN-8 | LOXIMVI | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 291.53 | p-Rb(S807/S811) | 246.99 | p-S6(S235/S236 | 502.86 | 1 | LOXIMVI-signal2 | D5 | |
| JNK-IN-8 | LOXIMVI | 0.0033 | uM | 24 | hr | | p27 Kip1 | 293.36 | p-Rb(S807/S811) | 201.67 | p-ERK(T202/Y204) | 200.98 | 2 | LOXIMVI-signal1 | E10 | |
| JNK-IN-8 | COLO 858 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 293.86 | p-Rb(S807/S811) | 167.77 | p-S6(S235/S236 | 396.05 | 1 | Colo858-signal2 | D9 | |
| JNK-IN-8 | LOXIMVI | 0.1280 | uM | 24 | hr | | p27 Kip1 | 297.55 | p-Rb(S807/S811) | 211.55 | p-ERK(T202/Y204) | 210.25 | 2 | LOXIMVI-signal1 | E6 | |
| JNK-IN-8 | LOXIMVI | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 297.60 | p-Rb(S807/S811) | 258.35 | p-S6(S235/S236 | 550.62 | 2 | LOXIMVI-signal2 | E4 | |
| Vemurafenib | LOXIMVI | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 298.38 | p-Rb(S807/S811) | 219.79 | p-S6(S235/S236 | 438.42 | 1 | LOXIMVI-signal2 | B8 | |
| JNK-IN-8 | LOXIMVI | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 299.84 | p-Rb(S807/S811) | 230.92 | p-S6(S235/S236 | 450.38 | 1 | LOXIMVI-signal2 | D8 | |
| JNK-IN-8 | LOXIMVI | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 299.94 | p-Rb(S807/S811) | 232.62 | p-S6(S235/S236 | 413.74 | 1 | LOXIMVI-signal2 | D11 | |
| JNK-IN-8 | LOXIMVI | 0.0082 | uM | 24 | hr | | p27 Kip1 | 300.54 | p-Rb(S807/S811) | 201.98 | p-ERK(T202/Y204) | 205.87 | 2 | LOXIMVI-signal1 | E9 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 300.67 | p-Rb(S807/S811) | 162.97 | p-S6(S235/S236 | 433.52 | 2 | Colo858-signal2 | G10 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 300.97 | p-Rb(S807/S811) | 216.71 | p-S6(S235/S236 | 433.18 | 2 | LOXIMVI-signal2 | G10 |
| Vemurafenib | WM-115 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 301.62 | p-Rb(S807/S811) | 38.59 | p-ERK(T202/Y204) | 24.54 | 2 | WM115-signal1 | C4 | |
| Vemurafenib | COLO 858 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 301.75 | p-Rb(S807/S811) | 164.37 | p-S6(S235/S236 | 394.95 | 2 | Colo858-signal2 | C9 | |
| JNK-IN-8 | COLO 858 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 302.14 | p-Rb(S807/S811) | 172.24 | p-S6(S235/S236 | 407.88 | 1 | Colo858-signal2 | D7 | |
| JNK-IN-8 | COLO 858 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 302.44 | p-Rb(S807/S811) | 181.09 | p-S6(S235/S236 | 433.45 | 2 | Colo858-signal2 | E5 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 302.92 | p-Rb(S807/S811) | 39.51 | p-ERK(T202/Y204) | 28.13 | 2 | WM115-signal1 | G4 |
| JNK-IN-8 | LOXIMVI | 0.0512 | uM | 24 | hr | | p27 Kip1 | 303.12 | p-Rb(S807/S811) | 207.96 | p-ERK(T202/Y204) | 204.10 | 2 | LOXIMVI-signal1 | E7 | |
| JNK-IN-8 | LOXIMVI | 0.0205 | uM | 24 | hr | | p27 Kip1 | 303.56 | p-Rb(S807/S811) | 209.54 | p-ERK(T202/Y204) | 206.46 | 1 | LOXIMVI-signal1 | D8 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 303.58 | p-Rb(S807/S811) | 202.43 | p-ERK(T202/Y204) | 184.11 | 2 | LOXIMVI-signal1 | G10 |
| Vemurafenib | LOXIMVI | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 304.49 | p-Rb(S807/S811) | 234.76 | p-S6(S235/S236 | 449.34 | 1 | LOXIMVI-signal2 | B11 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 304.49 | p-Rb(S807/S811) | 193.11 | p-ERK(T202/Y204) | 131.86 | 1 | LOXIMVI-signal1 | F8 |
| JNK-IN-8 | WM1552C | 5.0000 | uM | 24 | hr | | p27 Kip1 | 304.56 | p-Rb(S807/S811) | 111.24 | p-ERK(T202/Y204) | 317.45 | 1 | WM1552C-signal1 | D2 | |
| Vemurafenib | WM-115 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 304.70 | p-Rb(S807/S811) | 38.57 | p-ERK(T202/Y204) | 23.27 | 1 | WM115-signal1 | B4 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 306.56 | p-Rb(S807/S811) | 232.04 | p-S6(S235/S236 | 424.09 | 1 | LOXIMVI-signal2 | F11 |