Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | LOXIMVI | 5.0000 | uM | 24 | hr | | Ki-67 | 3004.40 | p-Rb(S807/S811) | 244.06 | p-cJun(S73) | 51.77 | 1 | LOXIMVI-signal3 | D2 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | Ki-67 | 2211.10 | p-Rb(S807/S811) | 96.70 | p-cJun(S73) | 24.08 | 2 | Colo858-signal3 | G2 |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 1101.80 | p-Rb(S807/S811) | 48.19 | p-ERK(T202/Y204) | 9.79 | 1 | Colo858-signal1 | B2 | |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 1100.60 | p-Rb(S807/S811) | 49.89 | p-ERK(T202/Y204) | 9.41 | 2 | Colo858-signal1 | C2 | |
| JNK-IN-8 | WM1552C | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 285.27 | p-Rb(S807/S811) | 112.32 | p-S6(S235/S236 | 447.61 | 2 | WM1552C-signal2 | E2 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | Ki-67 | 637.76 | p-Rb(S807/S811) | 61.90 | p-cJun(S73) | 19.77 | 2 | WM115-signal3 | G2 |
| JNK-IN-8 | LOXIMVI | 5.0000 | uM | 24 | hr | | Ki-67 | 2973.70 | p-Rb(S807/S811) | 240.23 | p-cJun(S73) | 48.04 | 2 | LOXIMVI-signal3 | E2 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | Ki-67 | 1967.00 | p-Rb(S807/S811) | 99.41 | p-cJun(S73) | 22.51 | 1 | Colo858-signal3 | F2 |
| JNK-IN-8 | LOXIMVI | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 388.24 | p-Rb(S807/S811) | 277.19 | p-S6(S235/S236 | 582.84 | 1 | LOXIMVI-signal2 | D2 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 397.69 | p-Rb(S807/S811) | 59.60 | p-ERK(T202/Y204) | 26.88 | 1 | WM1552C-signal1 | F2 |
| JNK-IN-8 | WM1552C | 5.0000 | uM | 24 | hr | | Ki-67 | 1547.50 | p-Rb(S807/S811) | 115.39 | p-cJun(S73) | 22.53 | 1 | WM1552C-signal3 | D2 | |
| Vemurafenib | WM1552C | 5.0000 | uM | 24 | hr | | Ki-67 | 973.61 | p-Rb(S807/S811) | 47.01 | p-cJun(S73) | 59.51 | 1 | WM1552C-signal3 | B2 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 5.0000 | uM | 24 | hr | | Ki-67 | 1101.80 | p-Rb(S807/S811) | 60.49 | p-cJun(S73) | 15.34 | 2 | WM1552C-signal3 | G2 |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 218.07 | p-Rb(S807/S811) | 73.50 | p-S6(S235/S236 | 96.41 | 2 | LOXIMVI-signal2 | C2 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 218.10 | p-Rb(S807/S811) | 64.53 | p-S6(S235/S236 | 16.11 | 1 | WM1552C-signal2 | F2 |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | p27 Kip1 | 499.44 | p-Rb(S807/S811) | 81.11 | p-ERK(T202/Y204) | 31.87 | 1 | LOXIMVI-signal1 | B2 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 5.0000 | uM | 24 | hr | | Ki-67 | 2814.00 | p-Rb(S807/S811) | 96.96 | p-cJun(S73) | 30.63 | 1 | LOXIMVI-signal3 | F2 |
| Vemurafenib | WM-115 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 349.44 | p-Rb(S807/S811) | 64.09 | p-ERK(T202/Y204) | 21.49 | 1 | WM115-signal1 | F2 |
| Vemurafenib | WM-115 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 209.18 | p-Rb(S807/S811) | 48.45 | p-S6(S235/S236 | 30.86 | 2 | WM115-signal2 | C2 | |
| JNK-IN-8 | WM1552C | 5.0000 | uM | 24 | hr | | p27 Kip1 | 313.64 | p-Rb(S807/S811) | 105.04 | p-ERK(T202/Y204) | 304.05 | 2 | WM1552C-signal1 | E2 |