Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | WM-115 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 230.19 | p-Rb(S807/S811) | 77.30 | p-ERK(T202/Y204) | 802.24 | 1 | WM115-signal1 | D2 | |
| Vemurafenib | WM1552C | 5.0000 | uM | 24 | hr | | p27 Kip1 | 446.61 | p-Rb(S807/S811) | 44.76 | p-ERK(T202/Y204) | 19.95 | 1 | WM1552C-signal1 | B2 | |
| JNK-IN-8 | WM1552C | 5.0000 | uM | 24 | hr | | Ki-67 | 1507.90 | p-Rb(S807/S811) | 107.69 | p-cJun(S73) | 21.50 | 2 | WM1552C-signal3 | E2 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 416.80 | p-Rb(S807/S811) | 107.61 | p-S6(S235/S236 | 19.41 | 1 | Colo858-signal2 | F2 |
| Vemurafenib | WM-115 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 231.89 | p-Rb(S807/S811) | 45.77 | p-S6(S235/S236 | 35.99 | 1 | WM115-signal2 | B2 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 5.0000 | uM | 24 | hr | | Ki-67 | 1057.80 | p-Rb(S807/S811) | 58.43 | p-cJun(S73) | 14.63 | 1 | WM1552C-signal3 | F2 |
| JNK-IN-8 | WM-115 | 5.0000 | uM | 24 | hr | | Ki-67 | 808.69 | p-Rb(S807/S811) | 76.27 | p-cJun(S73) | 22.54 | 2 | WM115-signal3 | E2 | |
| JNK-IN-8 | LOXIMVI | 5.0000 | uM | 24 | hr | | p27 Kip1 | 464.53 | p-Rb(S807/S811) | 268.11 | p-ERK(T202/Y204) | 267.97 | 1 | LOXIMVI-signal1 | D2 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 351.16 | p-Rb(S807/S811) | 100.40 | p-S6(S235/S236 | 22.18 | 2 | LOXIMVI-signal2 | G2 |
| Vemurafenib | WM1552C | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 184.35 | p-Rb(S807/S811) | 50.13 | p-S6(S235/S236 | 228.77 | 2 | WM1552C-signal2 | C2 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 1646.10 | p-Rb(S807/S811) | 108.76 | p-ERK(T202/Y204) | 29.73 | 1 | Colo858-signal1 | F2 |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | Ki-67 | 2047.60 | p-Rb(S807/S811) | 78.14 | p-cJun(S73) | 80.02 | 1 | LOXIMVI-signal3 | B2 | |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | Ki-67 | 947.93 | p-Rb(S807/S811) | 51.31 | p-cJun(S73) | 41.01 | 2 | Colo858-signal3 | C2 | |
| Vemurafenib | WM1552C | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 174.85 | p-Rb(S807/S811) | 52.10 | p-S6(S235/S236 | 261.40 | 1 | WM1552C-signal2 | B2 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 1526.80 | p-Rb(S807/S811) | 101.09 | p-ERK(T202/Y204) | 29.24 | 2 | Colo858-signal1 | G2 |
| JNK-IN-8 | WM-115 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 248.53 | p-Rb(S807/S811) | 77.96 | p-S6(S235/S236 | 209.55 | 1 | WM115-signal2 | D2 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 358.85 | p-Rb(S807/S811) | 104.29 | p-S6(S235/S236 | 19.60 | 1 | LOXIMVI-signal2 | F2 |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | Ki-67 | 1979.00 | p-Rb(S807/S811) | 73.51 | p-cJun(S73) | 77.14 | 2 | LOXIMVI-signal3 | C2 | |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | p27 Kip1 | 487.55 | p-Rb(S807/S811) | 84.67 | p-ERK(T202/Y204) | 31.63 | 2 | LOXIMVI-signal1 | C2 | |
| JNK-IN-8 | WM1552C | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 236.28 | p-Rb(S807/S811) | 113.21 | p-S6(S235/S236 | 450.52 | 1 | WM1552C-signal2 | D2 | |
| JNK-IN-8 | WM-115 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 267.33 | p-Rb(S807/S811) | 79.84 | p-ERK(T202/Y204) | 786.46 | 2 | WM115-signal1 | E2 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 418.63 | p-Rb(S807/S811) | 52.14 | p-ERK(T202/Y204) | 23.96 | 2 | WM1552C-signal1 | G2 |
| Vemurafenib | WM-115 | 5.0000 | uM | 24 | hr | | Ki-67 | 635.32 | p-Rb(S807/S811) | 49.14 | p-cJun(S73) | 49.50 | 2 | WM115-signal3 | C2 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 525.79 | p-Rb(S807/S811) | 128.17 | p-S6(S235/S236 | 40.46 | 2 | Colo858-signal2 | G2 |
| JNK-IN-8 | LOXIMVI | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 394.33 | p-Rb(S807/S811) | 290.15 | p-S6(S235/S236 | 433.67 | 2 | LOXIMVI-signal2 | E2 |