Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | LOXIMVI | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 297.60 | p-Rb(S807/S811) | 258.35 | p-S6(S235/S236 | 550.62 | 2 | LOXIMVI-signal2 | E4 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 229.22 | p-Rb(S807/S811) | 100.85 | p-S6(S235/S236 | 225.60 | 1 | LOXIMVI-signal2 | F4 |
| Vemurafenib | LOXIMVI | 0.8000 | uM | 24 | hr | | Ki-67 | 2357.40 | p-Rb(S807/S811) | 128.12 | p-cJun(S73) | 82.54 | 1 | LOXIMVI-signal3 | B4 | |
| JNK-IN-8 | WM-115 | 0.8000 | uM | 24 | hr | | Ki-67 | 800.51 | p-Rb(S807/S811) | 84.77 | p-cJun(S73) | 21.81 | 2 | WM115-signal3 | E4 | |
| JNK-IN-8 | COLO 858 | 0.8000 | uM | 24 | hr | | Ki-67 | 2394.30 | p-Rb(S807/S811) | 184.89 | p-cJun(S73) | 30.67 | 2 | Colo858-signal3 | E4 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 1044.90 | p-Rb(S807/S811) | 48.21 | p-ERK(T202/Y204) | 22.38 | 2 | Colo858-signal1 | G4 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.8000 | uM | 24 | hr | | Ki-67 | 1036.20 | p-Rb(S807/S811) | 55.60 | p-cJun(S73) | 34.11 | 2 | WM1552C-signal3 | G4 |
| Vemurafenib | WM1552C | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 153.80 | p-Rb(S807/S811) | 53.66 | p-S6(S235/S236 | 392.09 | 1 | WM1552C-signal2 | B4 | |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 152.45 | p-Rb(S807/S811) | 55.46 | p-S6(S235/S236 | 57.68 | 2 | Colo858-signal2 | C4 | |
| Vemurafenib | WM1552C | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 177.12 | p-Rb(S807/S811) | 56.01 | p-S6(S235/S236 | 356.27 | 2 | WM1552C-signal2 | C4 | |
| Vemurafenib | LOXIMVI | 0.8000 | uM | 24 | hr | | p27 Kip1 | 437.65 | p-Rb(S807/S811) | 139.21 | p-ERK(T202/Y204) | 46.29 | 1 | LOXIMVI-signal1 | B4 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 258.89 | p-Rb(S807/S811) | 123.29 | p-S6(S235/S236 | 272.97 | 2 | LOXIMVI-signal2 | G4 |
| Vemurafenib | LOXIMVI | 0.8000 | uM | 24 | hr | | p27 Kip1 | 434.48 | p-Rb(S807/S811) | 132.48 | p-ERK(T202/Y204) | 43.96 | 2 | LOXIMVI-signal1 | C4 | |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 1014.60 | p-Rb(S807/S811) | 48.01 | p-ERK(T202/Y204) | 18.67 | 2 | Colo858-signal1 | C4 | |
| JNK-IN-8 | WM-115 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 243.86 | p-Rb(S807/S811) | 88.86 | p-ERK(T202/Y204) | 663.72 | 1 | WM115-signal1 | D4 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 168.97 | p-Rb(S807/S811) | 57.67 | p-S6(S235/S236 | 47.25 | 1 | Colo858-signal2 | F4 |
| Vemurafenib | WM-115 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 179.29 | p-Rb(S807/S811) | 38.89 | p-S6(S235/S236 | 65.75 | 2 | WM115-signal2 | C4 | |
| JNK-IN-8 | LOXIMVI | 0.8000 | uM | 24 | hr | | p27 Kip1 | 336.96 | p-Rb(S807/S811) | 231.40 | p-ERK(T202/Y204) | 222.11 | 1 | LOXIMVI-signal1 | D4 | |
| JNK-IN-8 | WM1552C | 0.8000 | uM | 24 | hr | | Ki-67 | 1751.50 | p-Rb(S807/S811) | 114.25 | p-cJun(S73) | 56.72 | 2 | WM1552C-signal3 | E4 | |
| Vemurafenib | WM1552C | 0.8000 | uM | 24 | hr | | p27 Kip1 | 442.40 | p-Rb(S807/S811) | 51.64 | p-ERK(T202/Y204) | 26.95 | 1 | WM1552C-signal1 | B4 | |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | Ki-67 | 678.24 | p-Rb(S807/S811) | 45.98 | p-cJun(S73) | 72.90 | 1 | Colo858-signal3 | B4 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 302.92 | p-Rb(S807/S811) | 39.51 | p-ERK(T202/Y204) | 28.13 | 2 | WM115-signal1 | G4 |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 165.33 | p-Rb(S807/S811) | 58.60 | p-S6(S235/S236 | 48.12 | 1 | Colo858-signal2 | B4 | |
| JNK-IN-8 | WM-115 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 235.96 | p-Rb(S807/S811) | 79.36 | p-S6(S235/S236 | 186.75 | 1 | WM115-signal2 | D4 | |
| Vemurafenib | WM-115 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 301.62 | p-Rb(S807/S811) | 38.59 | p-ERK(T202/Y204) | 24.54 | 2 | WM115-signal1 | C4 |