Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 125.76 | p-Rb(S807/S811) | 58.82 | p-S6(S235/S236 | 110.32 | 2 | Colo858-signal2 | G5 |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 150.91 | p-Rb(S807/S811) | 54.62 | p-S6(S235/S236 | 93.11 | 2 | Colo858-signal2 | C5 | |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | Ki-67 | 708.08 | p-Rb(S807/S811) | 48.09 | p-cJun(S73) | 72.08 | 1 | Colo858-signal3 | B5 | |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | Ki-67 | 660.32 | p-Rb(S807/S811) | 48.67 | p-cJun(S73) | 67.95 | 2 | Colo858-signal3 | C5 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 182.61 | p-Rb(S807/S811) | 38.78 | p-S6(S235/S236 | 51.74 | 2 | WM115-signal2 | G4 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.8000 | uM | 24 | hr | | Ki-67 | 2249.30 | p-Rb(S807/S811) | 93.65 | p-cJun(S73) | 24.47 | 1 | LOXIMVI-signal3 | F4 |
| JNK-IN-8 | WM-115 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 235.37 | p-Rb(S807/S811) | 88.77 | p-ERK(T202/Y204) | 657.24 | 2 | WM115-signal1 | E4 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 158.30 | p-Rb(S807/S811) | 60.54 | p-S6(S235/S236 | 363.25 | 1 | WM1552C-signal2 | F4 |
| Vemurafenib | LOXIMVI | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 248.35 | p-Rb(S807/S811) | 129.91 | p-S6(S235/S236 | 357.36 | 2 | LOXIMVI-signal2 | C4 | |
| JNK-IN-8 | LOXIMVI | 0.8000 | uM | 24 | hr | | Ki-67 | 3017.80 | p-Rb(S807/S811) | 223.34 | p-cJun(S73) | 56.18 | 2 | LOXIMVI-signal3 | E4 | |
| Vemurafenib | WM1552C | 0.8000 | uM | 24 | hr | | Ki-67 | 1017.40 | p-Rb(S807/S811) | 54.97 | p-cJun(S73) | 137.70 | 2 | WM1552C-signal3 | C4 | |
| JNK-IN-8 | WM-115 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 232.27 | p-Rb(S807/S811) | 78.02 | p-S6(S235/S236 | 185.29 | 2 | WM115-signal2 | E4 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 428.03 | p-Rb(S807/S811) | 51.23 | p-ERK(T202/Y204) | 26.59 | 1 | WM1552C-signal1 | F4 |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | Ki-67 | 680.51 | p-Rb(S807/S811) | 40.34 | p-cJun(S73) | 71.07 | 2 | Colo858-signal3 | C4 | |
| JNK-IN-8 | COLO 858 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 488.74 | p-Rb(S807/S811) | 190.14 | p-ERK(T202/Y204) | 694.83 | 1 | Colo858-signal1 | D4 | |
| JNK-IN-8 | WM1552C | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 204.00 | p-Rb(S807/S811) | 111.59 | p-S6(S235/S236 | 385.25 | 2 | WM1552C-signal2 | E4 | |
| JNK-IN-8 | LOXIMVI | 0.8000 | uM | 24 | hr | | Ki-67 | 2971.40 | p-Rb(S807/S811) | 219.05 | p-cJun(S73) | 57.05 | 1 | LOXIMVI-signal3 | D4 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 164.14 | p-Rb(S807/S811) | 55.90 | p-S6(S235/S236 | 45.82 | 2 | Colo858-signal2 | G4 |
| JNK-IN-8 | LOXIMVI | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 344.33 | p-Rb(S807/S811) | 248.42 | p-S6(S235/S236 | 524.11 | 1 | LOXIMVI-signal2 | D4 | |
| Vemurafenib | LOXIMVI | 0.8000 | uM | 24 | hr | | Ki-67 | 2448.30 | p-Rb(S807/S811) | 126.79 | p-cJun(S73) | 81.56 | 2 | LOXIMVI-signal3 | C4 | |
| JNK-IN-8 | WM1552C | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 262.28 | p-Rb(S807/S811) | 113.37 | p-S6(S235/S236 | 413.92 | 1 | WM1552C-signal2 | D4 | |
| JNK-IN-8 | COLO 858 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 504.81 | p-Rb(S807/S811) | 190.17 | p-ERK(T202/Y204) | 719.29 | 2 | Colo858-signal1 | E4 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 421.07 | p-Rb(S807/S811) | 53.69 | p-ERK(T202/Y204) | 24.84 | 2 | WM1552C-signal1 | G4 |
| Vemurafenib | WM1552C | 0.8000 | uM | 24 | hr | | Ki-67 | 1044.70 | p-Rb(S807/S811) | 52.94 | p-cJun(S73) | 128.12 | 1 | WM1552C-signal3 | B4 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.8000 | uM | 24 | hr | | Ki-67 | 682.82 | p-Rb(S807/S811) | 40.64 | p-cJun(S73) | 20.31 | 2 | WM115-signal3 | G4 |