Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | LOXIMVI | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 260.69 | p-Rb(S807/S811) | 241.93 | p-S6(S235/S236 | 501.67 | 2 | LOXIMVI-signal2 | E5 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 983.23 | p-Rb(S807/S811) | 56.72 | p-ERK(T202/Y204) | 28.24 | 2 | Colo858-signal1 | G5 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 237.24 | p-Rb(S807/S811) | 135.99 | p-S6(S235/S236 | 359.46 | 1 | LOXIMVI-signal2 | F5 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 233.71 | p-Rb(S807/S811) | 136.67 | p-S6(S235/S236 | 375.36 | 2 | LOXIMVI-signal2 | G5 |
| Vemurafenib | LOXIMVI | 0.3200 | uM | 24 | hr | | Ki-67 | 2470.00 | p-Rb(S807/S811) | 138.22 | p-cJun(S73) | 75.91 | 1 | LOXIMVI-signal3 | B5 | |
| Vemurafenib | LOXIMVI | 0.3200 | uM | 24 | hr | | Ki-67 | 2387.10 | p-Rb(S807/S811) | 134.09 | p-cJun(S73) | 83.10 | 2 | LOXIMVI-signal3 | C5 | |
| JNK-IN-8 | LOXIMVI | 0.3200 | uM | 24 | hr | | Ki-67 | 2939.70 | p-Rb(S807/S811) | 217.05 | p-cJun(S73) | 57.83 | 1 | LOXIMVI-signal3 | D5 | |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 964.47 | p-Rb(S807/S811) | 50.08 | p-ERK(T202/Y204) | 25.09 | 1 | Colo858-signal1 | B5 | |
| JNK-IN-8 | LOXIMVI | 0.3200 | uM | 24 | hr | | Ki-67 | 2848.50 | p-Rb(S807/S811) | 207.66 | p-cJun(S73) | 57.32 | 2 | LOXIMVI-signal3 | E5 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.3200 | uM | 24 | hr | | Ki-67 | 2320.10 | p-Rb(S807/S811) | 113.12 | p-cJun(S73) | 29.40 | 1 | LOXIMVI-signal3 | F5 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.3200 | uM | 24 | hr | | Ki-67 | 2301.20 | p-Rb(S807/S811) | 115.21 | p-cJun(S73) | 27.54 | 2 | LOXIMVI-signal3 | G5 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 1024.70 | p-Rb(S807/S811) | 48.87 | p-ERK(T202/Y204) | 30.22 | 1 | Colo858-signal1 | F5 |
| Vemurafenib | WM-115 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 263.18 | p-Rb(S807/S811) | 37.97 | p-ERK(T202/Y204) | 43.14 | 1 | WM115-signal1 | B5 | |
| JNK-IN-8 | WM1552C | 0.3200 | uM | 24 | hr | | Ki-67 | 1583.90 | p-Rb(S807/S811) | 110.94 | p-cJun(S73) | 105.82 | 1 | WM1552C-signal3 | D5 | |
| Vemurafenib | WM-115 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 257.65 | p-Rb(S807/S811) | 38.40 | p-ERK(T202/Y204) | 44.96 | 2 | WM115-signal1 | C5 | |
| JNK-IN-8 | WM-115 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 236.84 | p-Rb(S807/S811) | 83.75 | p-ERK(T202/Y204) | 640.13 | 1 | WM115-signal1 | D5 | |
| JNK-IN-8 | WM-115 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 232.47 | p-Rb(S807/S811) | 84.23 | p-ERK(T202/Y204) | 633.23 | 2 | WM115-signal1 | E5 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 277.07 | p-Rb(S807/S811) | 41.08 | p-ERK(T202/Y204) | 52.64 | 1 | WM115-signal1 | F5 |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 277.38 | p-Rb(S807/S811) | 42.72 | p-ERK(T202/Y204) | 54.07 | 2 | WM115-signal1 | G5 |
| Vemurafenib | WM-115 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 180.39 | p-Rb(S807/S811) | 38.47 | p-S6(S235/S236 | 77.58 | 1 | WM115-signal2 | B5 | |
| Vemurafenib | WM-115 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 148.64 | p-Rb(S807/S811) | 35.95 | p-S6(S235/S236 | 64.00 | 2 | WM115-signal2 | C5 | |
| JNK-IN-8 | COLO 858 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 445.16 | p-Rb(S807/S811) | 182.28 | p-ERK(T202/Y204) | 673.10 | 2 | Colo858-signal1 | E5 | |
| JNK-IN-8 | WM-115 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 205.29 | p-Rb(S807/S811) | 76.65 | p-S6(S235/S236 | 177.08 | 1 | WM115-signal2 | D5 | |
| JNK-IN-8 | WM-115 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 260.22 | p-Rb(S807/S811) | 76.23 | p-S6(S235/S236 | 189.51 | 2 | WM115-signal2 | E5 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 206.61 | p-Rb(S807/S811) | 41.17 | p-S6(S235/S236 | 77.73 | 1 | WM115-signal2 | F5 |