Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | LOXIMVI | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 281.63 | p-Rb(S807/S811) | 231.63 | p-S6(S235/S236 | 455.97 | 2 | LOXIMVI-signal2 | E8 | |
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | Ki-67 | 2138.80 | p-Rb(S807/S811) | 144.45 | p-cJun(S73) | 30.43 | 2 | Colo858-signal3 | C8 | |
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 307.92 | p-Rb(S807/S811) | 162.01 | p-S6(S235/S236 | 403.15 | 1 | Colo858-signal2 | B8 | |
| Vemurafenib | WM-115 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 230.38 | p-Rb(S807/S811) | 73.32 | p-ERK(T202/Y204) | 501.50 | 2 | WM115-signal1 | C8 | |
| Vemurafenib | WM1552C | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 223.26 | p-Rb(S807/S811) | 102.60 | p-S6(S235/S236 | 381.13 | 2 | WM1552C-signal2 | C8 | |
| JNK-IN-8 | LOXIMVI | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 299.84 | p-Rb(S807/S811) | 230.92 | p-S6(S235/S236 | 450.38 | 1 | LOXIMVI-signal2 | D8 | |
| Vemurafenib | WM-115 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 196.19 | p-Rb(S807/S811) | 67.83 | p-S6(S235/S236 | 199.48 | 1 | WM115-signal2 | B8 | |
| JNK-IN-8 | WM-115 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 216.44 | p-Rb(S807/S811) | 71.32 | p-S6(S235/S236 | 187.08 | 1 | WM115-signal2 | D8 | |
| Vemurafenib | LOXIMVI | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 265.14 | p-Rb(S807/S811) | 204.08 | p-S6(S235/S236 | 419.00 | 2 | LOXIMVI-signal2 | C8 | |
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | Ki-67 | 2103.40 | p-Rb(S807/S811) | 149.11 | p-cJun(S73) | 30.17 | 1 | Colo858-signal3 | B8 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | Ki-67 | 795.90 | p-Rb(S807/S811) | 74.91 | p-cJun(S73) | 44.16 | 1 | WM115-signal3 | F8 |
| Vemurafenib | WM1552C | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 181.99 | p-Rb(S807/S811) | 102.95 | p-S6(S235/S236 | 423.14 | 1 | WM1552C-signal2 | B8 | |
| JNK-IN-8 | WM1552C | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 244.72 | p-Rb(S807/S811) | 115.35 | p-S6(S235/S236 | 425.96 | 1 | WM1552C-signal2 | D8 | |
| Vemurafenib | LOXIMVI | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 255.47 | p-Rb(S807/S811) | 192.91 | p-S6(S235/S236 | 440.25 | 1 | LOXIMVI-signal2 | B7 | |
| Vemurafenib | WM-115 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 237.09 | p-Rb(S807/S811) | 68.88 | p-ERK(T202/Y204) | 366.99 | 1 | WM115-signal1 | B7 | |
| JNK-IN-8 | LOXIMVI | 0.0512 | uM | 24 | hr | | Ki-67 | 2947.60 | p-Rb(S807/S811) | 217.27 | p-cJun(S73) | 73.06 | 1 | LOXIMVI-signal3 | D7 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 325.15 | p-Rb(S807/S811) | 174.20 | p-ERK(T202/Y204) | 85.62 | 2 | LOXIMVI-signal1 | G7 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 239.08 | p-Rb(S807/S811) | 140.42 | p-S6(S235/S236 | 380.85 | 2 | Colo858-signal2 | G7 |
| Vemurafenib | WM-115 | 0.0512 | uM | 24 | hr | | Ki-67 | 753.40 | p-Rb(S807/S811) | 69.46 | p-cJun(S73) | 57.69 | 1 | WM115-signal3 | B7 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 235.54 | p-Rb(S807/S811) | 72.02 | p-ERK(T202/Y204) | 366.04 | 2 | WM115-signal1 | G7 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 511.00 | p-Rb(S807/S811) | 125.23 | p-ERK(T202/Y204) | 254.95 | 2 | Colo858-signal1 | G7 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 268.49 | p-Rb(S807/S811) | 89.43 | p-ERK(T202/Y204) | 154.75 | 1 | WM1552C-signal1 | F7 |
| JNK-IN-8 | WM1552C | 0.0512 | uM | 24 | hr | | p27 Kip1 | 242.80 | p-Rb(S807/S811) | 108.49 | p-ERK(T202/Y204) | 267.00 | 2 | WM1552C-signal1 | E7 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 338.89 | p-Rb(S807/S811) | 176.50 | p-ERK(T202/Y204) | 86.35 | 1 | LOXIMVI-signal1 | F7 |
| JNK-IN-8 | COLO 858 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 430.28 | p-Rb(S807/S811) | 171.77 | p-ERK(T202/Y204) | 673.40 | 1 | Colo858-signal1 | D7 |