Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | Ki-67 | 2064.40 | p-Rb(S807/S811) | 152.34 | p-cJun(S73) | 26.96 | 2 | Colo858-signal3 | G8 |
| JNK-IN-8 | COLO 858 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 288.25 | p-Rb(S807/S811) | 170.31 | p-S6(S235/S236 | 401.43 | 1 | Colo858-signal2 | D8 | |
| Vemurafenib | WM1552C | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 223.26 | p-Rb(S807/S811) | 102.60 | p-S6(S235/S236 | 381.13 | 2 | WM1552C-signal2 | C8 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 245.42 | p-Rb(S807/S811) | 98.27 | p-ERK(T202/Y204) | 209.11 | 2 | WM1552C-signal1 | G8 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | Ki-67 | 2146.20 | p-Rb(S807/S811) | 150.60 | p-cJun(S73) | 28.79 | 1 | Colo858-signal3 | F8 |
| Vemurafenib | LOXIMVI | 0.0205 | uM | 24 | hr | | Ki-67 | 2858.70 | p-Rb(S807/S811) | 191.23 | p-cJun(S73) | 102.21 | 2 | LOXIMVI-signal3 | C8 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0205 | uM | 24 | hr | | Ki-67 | 2617.50 | p-Rb(S807/S811) | 180.39 | p-cJun(S73) | 69.18 | 2 | LOXIMVI-signal3 | G8 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 267.75 | p-Rb(S807/S811) | 208.98 | p-S6(S235/S236 | 429.59 | 1 | LOXIMVI-signal2 | F8 |
| Vemurafenib | WM-115 | 0.0205 | uM | 24 | hr | | Ki-67 | 780.78 | p-Rb(S807/S811) | 72.14 | p-cJun(S73) | 50.93 | 1 | WM115-signal3 | B8 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 223.67 | p-Rb(S807/S811) | 74.26 | p-ERK(T202/Y204) | 488.45 | 2 | WM115-signal1 | G8 |
| JNK-IN-8 | COLO 858 | 0.0205 | uM | 24 | hr | | Ki-67 | 2269.90 | p-Rb(S807/S811) | 156.97 | p-cJun(S73) | 29.44 | 2 | Colo858-signal3 | E8 | |
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 349.40 | p-Rb(S807/S811) | 166.03 | p-S6(S235/S236 | 385.58 | 2 | Colo858-signal2 | C8 | |
| JNK-IN-8 | COLO 858 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 437.19 | p-Rb(S807/S811) | 168.12 | p-ERK(T202/Y204) | 644.13 | 2 | Colo858-signal1 | E8 | |
| JNK-IN-8 | WM1552C | 0.0205 | uM | 24 | hr | | p27 Kip1 | 245.39 | p-Rb(S807/S811) | 107.49 | p-ERK(T202/Y204) | 270.03 | 2 | WM1552C-signal1 | E8 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 227.51 | p-Rb(S807/S811) | 77.75 | p-ERK(T202/Y204) | 515.73 | 1 | WM115-signal1 | F8 |
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 483.48 | p-Rb(S807/S811) | 154.86 | p-ERK(T202/Y204) | 515.79 | 2 | Colo858-signal1 | C8 | |
| JNK-IN-8 | COLO 858 | 0.0205 | uM | 24 | hr | | Ki-67 | 2267.30 | p-Rb(S807/S811) | 156.38 | p-cJun(S73) | 31.07 | 1 | Colo858-signal3 | D8 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | p-Rb(S807/S811) | p-S6(S235/S236 | 1 | WM115-signal2 | F8 | |||
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 202.11 | p-Rb(S807/S811) | 70.44 | p-S6(S235/S236 | 160.70 | 2 | WM115-signal2 | G8 |
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 496.15 | p-Rb(S807/S811) | 149.24 | p-ERK(T202/Y204) | 495.70 | 1 | Colo858-signal1 | B8 | |
| JNK-IN-8 | WM-115 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 228.02 | p-Rb(S807/S811) | 77.98 | p-ERK(T202/Y204) | 657.81 | 2 | WM115-signal1 | E8 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 248.11 | p-Rb(S807/S811) | 100.68 | p-ERK(T202/Y204) | 212.80 | 1 | WM1552C-signal1 | F8 |
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | Ki-67 | 2138.80 | p-Rb(S807/S811) | 144.45 | p-cJun(S73) | 30.43 | 2 | Colo858-signal3 | C8 | |
| JNK-IN-8 | WM1552C | 0.0205 | uM | 24 | hr | | p27 Kip1 | 252.37 | p-Rb(S807/S811) | 110.39 | p-ERK(T202/Y204) | 260.36 | 1 | WM1552C-signal1 | D8 | |
| Vemurafenib | WM1552C | 0.0205 | uM | 24 | hr | | p27 Kip1 | 249.99 | p-Rb(S807/S811) | 102.20 | p-ERK(T202/Y204) | 212.75 | 2 | WM1552C-signal1 | C8 |