Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 304.49 | p-Rb(S807/S811) | 193.11 | p-ERK(T202/Y204) | 131.86 | 1 | LOXIMVI-signal1 | F8 |
| JNK-IN-8 | WM-115 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 228.02 | p-Rb(S807/S811) | 77.98 | p-ERK(T202/Y204) | 657.81 | 2 | WM115-signal1 | E8 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0205 | uM | 24 | hr | | Ki-67 | 2734.80 | p-Rb(S807/S811) | 188.75 | p-cJun(S73) | 65.47 | 1 | LOXIMVI-signal3 | F8 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 245.42 | p-Rb(S807/S811) | 98.27 | p-ERK(T202/Y204) | 209.11 | 2 | WM1552C-signal1 | G8 |
| JNK-IN-8 | LOXIMVI | 0.0205 | uM | 24 | hr | | p27 Kip1 | 286.32 | p-Rb(S807/S811) | 204.47 | p-ERK(T202/Y204) | 198.65 | 2 | LOXIMVI-signal1 | E8 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 337.75 | p-Rb(S807/S811) | 156.22 | p-S6(S235/S236 | 357.37 | 1 | Colo858-signal2 | F8 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 248.11 | p-Rb(S807/S811) | 100.68 | p-ERK(T202/Y204) | 212.80 | 1 | WM1552C-signal1 | F8 |
| JNK-IN-8 | LOXIMVI | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 299.84 | p-Rb(S807/S811) | 230.92 | p-S6(S235/S236 | 450.38 | 1 | LOXIMVI-signal2 | D8 | |
| JNK-IN-8 | WM-115 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 235.89 | p-Rb(S807/S811) | 78.16 | p-ERK(T202/Y204) | 655.44 | 1 | WM115-signal1 | D8 | |
| JNK-IN-8 | WM1552C | 0.0205 | uM | 24 | hr | | p27 Kip1 | 245.39 | p-Rb(S807/S811) | 107.49 | p-ERK(T202/Y204) | 270.03 | 2 | WM1552C-signal1 | E8 | |
| JNK-IN-8 | LOXIMVI | 0.0205 | uM | 24 | hr | | p27 Kip1 | 303.56 | p-Rb(S807/S811) | 209.54 | p-ERK(T202/Y204) | 206.46 | 1 | LOXIMVI-signal1 | D8 | |
| JNK-IN-8 | WM1552C | 0.0205 | uM | 24 | hr | | p27 Kip1 | 252.37 | p-Rb(S807/S811) | 110.39 | p-ERK(T202/Y204) | 260.36 | 1 | WM1552C-signal1 | D8 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 317.63 | p-Rb(S807/S811) | 191.25 | p-ERK(T202/Y204) | 128.24 | 2 | LOXIMVI-signal1 | G8 |
| Vemurafenib | LOXIMVI | 0.0205 | uM | 24 | hr | | p27 Kip1 | 319.35 | p-Rb(S807/S811) | 195.10 | p-ERK(T202/Y204) | 133.04 | 2 | LOXIMVI-signal1 | C8 | |
| JNK-IN-8 | COLO 858 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 311.93 | p-Rb(S807/S811) | 166.54 | p-S6(S235/S236 | 387.41 | 2 | Colo858-signal2 | E8 | |
| Vemurafenib | WM-115 | 0.0205 | uM | 24 | hr | | Ki-67 | 761.10 | p-Rb(S807/S811) | 71.98 | p-cJun(S73) | 49.80 | 2 | WM115-signal3 | C8 | |
| JNK-IN-8 | LOXIMVI | 0.0205 | uM | 24 | hr | | Ki-67 | 2981.20 | p-Rb(S807/S811) | 212.09 | p-cJun(S73) | 82.93 | 2 | LOXIMVI-signal3 | E8 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 462.46 | p-Rb(S807/S811) | 154.40 | p-ERK(T202/Y204) | 499.08 | 1 | Colo858-signal1 | F8 |
| JNK-IN-8 | WM-115 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 223.09 | p-Rb(S807/S811) | 70.57 | p-S6(S235/S236 | 184.86 | 2 | WM115-signal2 | E8 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | p-Rb(S807/S811) | p-S6(S235/S236 | 1 | WM115-signal2 | F8 | |||
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 258.76 | p-Rb(S807/S811) | 210.04 | p-S6(S235/S236 | 429.29 | 2 | LOXIMVI-signal2 | G8 |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 202.11 | p-Rb(S807/S811) | 70.44 | p-S6(S235/S236 | 160.70 | 2 | WM115-signal2 | G8 |
| Vemurafenib | WM1552C | 0.0205 | uM | 24 | hr | | p27 Kip1 | 252.92 | p-Rb(S807/S811) | 100.14 | p-ERK(T202/Y204) | 207.93 | 1 | WM1552C-signal1 | B8 | |
| Vemurafenib | LOXIMVI | 0.0205 | uM | 24 | hr | | p27 Kip1 | 330.03 | p-Rb(S807/S811) | 203.83 | p-ERK(T202/Y204) | 137.92 | 1 | LOXIMVI-signal1 | B8 | |
| JNK-IN-8 | WM-115 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 216.44 | p-Rb(S807/S811) | 71.32 | p-S6(S235/S236 | 187.08 | 1 | WM115-signal2 | D8 |