Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | COLO 858 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 357.02 | p-Rb(S807/S811) | 174.95 | p-S6(S235/S236 | 420.53 | 2 | Colo858-signal2 | E11 | |
| Vemurafenib | COLO 858 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 1027.10 | p-Rb(S807/S811) | 46.02 | p-ERK(T202/Y204) | 14.33 | 2 | Colo858-signal1 | C3 | |
| JNK-IN-8 | COLO 858 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 343.78 | p-Rb(S807/S811) | 196.27 | p-S6(S235/S236 | 463.89 | 2 | Colo858-signal2 | E4 | |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 1014.60 | p-Rb(S807/S811) | 48.01 | p-ERK(T202/Y204) | 18.67 | 2 | Colo858-signal1 | C4 | |
| JNK-IN-8 | COLO 858 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 442.59 | p-Rb(S807/S811) | 160.97 | p-ERK(T202/Y204) | 615.32 | 2 | Colo858-signal1 | E10 | |
| JNK-IN-8 | COLO 858 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 445.15 | p-Rb(S807/S811) | 158.36 | p-ERK(T202/Y204) | 601.12 | 2 | Colo858-signal1 | E11 | |
| JNK-IN-8 | COLO 858 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 839.15 | p-Rb(S807/S811) | 256.43 | p-ERK(T202/Y204) | 789.95 | 2 | Colo858-signal1 | E2 | |
| JNK-IN-8 | COLO 858 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 594.04 | p-Rb(S807/S811) | 231.49 | p-ERK(T202/Y204) | 724.41 | 2 | Colo858-signal1 | E3 | |
| JNK-IN-8 | COLO 858 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 504.81 | p-Rb(S807/S811) | 190.17 | p-ERK(T202/Y204) | 719.29 | 2 | Colo858-signal1 | E4 | |
| JNK-IN-8 | COLO 858 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 445.16 | p-Rb(S807/S811) | 182.28 | p-ERK(T202/Y204) | 673.10 | 2 | Colo858-signal1 | E5 | |
| JNK-IN-8 | COLO 858 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 429.89 | p-Rb(S807/S811) | 170.46 | p-ERK(T202/Y204) | 675.31 | 2 | Colo858-signal1 | E6 | |
| JNK-IN-8 | COLO 858 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 429.12 | p-Rb(S807/S811) | 167.32 | p-ERK(T202/Y204) | 661.59 | 2 | Colo858-signal1 | E7 | |
| JNK-IN-8 | COLO 858 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 437.19 | p-Rb(S807/S811) | 168.12 | p-ERK(T202/Y204) | 644.13 | 2 | Colo858-signal1 | E8 | |
| JNK-IN-8 | COLO 858 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 438.93 | p-Rb(S807/S811) | 156.13 | p-ERK(T202/Y204) | 620.57 | 2 | Colo858-signal1 | E9 | |
| Vemurafenib | COLO 858 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 461.92 | p-Rb(S807/S811) | 163.29 | p-ERK(T202/Y204) | 613.35 | 2 | Colo858-signal1 | C9 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 1044.90 | p-Rb(S807/S811) | 48.21 | p-ERK(T202/Y204) | 22.38 | 2 | Colo858-signal1 | G4 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 983.23 | p-Rb(S807/S811) | 56.72 | p-ERK(T202/Y204) | 28.24 | 2 | Colo858-signal1 | G5 |
| Vemurafenib | COLO 858 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 489.20 | p-Rb(S807/S811) | 160.66 | p-ERK(T202/Y204) | 628.14 | 2 | Colo858-signal1 | C10 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 458.83 | p-Rb(S807/S811) | 152.02 | p-ERK(T202/Y204) | 459.90 | 2 | Colo858-signal1 | G8 |
| Vemurafenib | COLO 858 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 254.30 | p-Rb(S807/S811) | 163.98 | p-S6(S235/S236 | 398.31 | 2 | Colo858-signal2 | C10 | |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 377.25 | p-Rb(S807/S811) | 161.02 | p-S6(S235/S236 | 380.19 | 2 | Colo858-signal2 | C11 | |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 452.92 | p-Rb(S807/S811) | 154.74 | p-ERK(T202/Y204) | 648.89 | 2 | Colo858-signal1 | C11 | |
| Vemurafenib | COLO 858 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 174.16 | p-Rb(S807/S811) | 73.24 | p-S6(S235/S236 | 166.46 | 2 | Colo858-signal2 | C6 | |
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 349.40 | p-Rb(S807/S811) | 166.03 | p-S6(S235/S236 | 385.58 | 2 | Colo858-signal2 | C8 | |
| JNK-IN-8 | COLO 858 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 373.77 | p-Rb(S807/S811) | 163.58 | p-S6(S235/S236 | 386.62 | 2 | Colo858-signal2 | E9 |