Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | WM-115 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 235.79 | p-Rb(S807/S811) | 81.08 | p-ERK(T202/Y204) | 651.65 | 2 | WM115-signal1 | E6 | |
| JNK-IN-8 | WM-115 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 242.63 | p-Rb(S807/S811) | 82.99 | p-ERK(T202/Y204) | 649.81 | 1 | WM115-signal1 | D7 | |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 452.92 | p-Rb(S807/S811) | 154.74 | p-ERK(T202/Y204) | 648.89 | 2 | Colo858-signal1 | C11 | |
| JNK-IN-8 | COLO 858 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 436.36 | p-Rb(S807/S811) | 155.67 | p-ERK(T202/Y204) | 648.15 | 1 | Colo858-signal1 | D9 | |
| JNK-IN-8 | COLO 858 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 437.19 | p-Rb(S807/S811) | 168.12 | p-ERK(T202/Y204) | 644.13 | 2 | Colo858-signal1 | E8 | |
| JNK-IN-8 | WM-115 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 236.84 | p-Rb(S807/S811) | 83.75 | p-ERK(T202/Y204) | 640.13 | 1 | WM115-signal1 | D5 | |
| JNK-IN-8 | WM-115 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 222.05 | p-Rb(S807/S811) | 76.04 | p-ERK(T202/Y204) | 636.67 | 2 | WM115-signal1 | E10 | |
| Vemurafenib | WM-115 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 242.95 | p-Rb(S807/S811) | 73.13 | p-ERK(T202/Y204) | 635.44 | 1 | WM115-signal1 | B11 | |
| JNK-IN-8 | WM-115 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 232.47 | p-Rb(S807/S811) | 84.23 | p-ERK(T202/Y204) | 633.23 | 2 | WM115-signal1 | E5 | |
| JNK-IN-8 | WM-115 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 238.03 | p-Rb(S807/S811) | 74.48 | p-ERK(T202/Y204) | 630.06 | 1 | WM115-signal1 | D11 | |
| JNK-IN-8 | COLO 858 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 454.18 | p-Rb(S807/S811) | 159.65 | p-ERK(T202/Y204) | 628.56 | 1 | Colo858-signal1 | D11 | |
| JNK-IN-8 | WM-115 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 225.01 | p-Rb(S807/S811) | 72.59 | p-ERK(T202/Y204) | 628.25 | 2 | WM115-signal1 | E11 | |
| Vemurafenib | COLO 858 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 489.20 | p-Rb(S807/S811) | 160.66 | p-ERK(T202/Y204) | 628.14 | 2 | Colo858-signal1 | C10 | |
| JNK-IN-8 | LOXIMVI | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 368.60 | p-Rb(S807/S811) | 272.58 | p-S6(S235/S236 | 627.74 | 1 | LOXIMVI-signal2 | D3 | |
| JNK-IN-8 | COLO 858 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 438.30 | p-Rb(S807/S811) | 160.10 | p-ERK(T202/Y204) | 626.02 | 1 | Colo858-signal1 | D10 | |
| JNK-IN-8 | WM-115 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 227.05 | p-Rb(S807/S811) | 74.53 | p-ERK(T202/Y204) | 625.81 | 1 | WM115-signal1 | D10 | |
| Vemurafenib | WM-115 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 238.61 | p-Rb(S807/S811) | 73.09 | p-ERK(T202/Y204) | 620.90 | 2 | WM115-signal1 | C11 | |
| JNK-IN-8 | COLO 858 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 315.08 | p-Rb(S807/S811) | 253.54 | p-S6(S235/S236 | 620.64 | 1 | Colo858-signal2 | D2 | |
| JNK-IN-8 | COLO 858 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 438.93 | p-Rb(S807/S811) | 156.13 | p-ERK(T202/Y204) | 620.57 | 2 | Colo858-signal1 | E9 | |
| JNK-IN-8 | LOXIMVI | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 377.73 | p-Rb(S807/S811) | 274.50 | p-S6(S235/S236 | 619.52 | 2 | LOXIMVI-signal2 | E3 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 228.13 | p-Rb(S807/S811) | 74.57 | p-ERK(T202/Y204) | 615.48 | 1 | WM115-signal1 | F11 |
| JNK-IN-8 | COLO 858 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 442.59 | p-Rb(S807/S811) | 160.97 | p-ERK(T202/Y204) | 615.32 | 2 | Colo858-signal1 | E10 | |
| JNK-IN-8 | COLO 858 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 370.03 | p-Rb(S807/S811) | 260.57 | p-S6(S235/S236 | 614.60 | 2 | Colo858-signal2 | E2 | |
| Vemurafenib | WM-115 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 224.32 | p-Rb(S807/S811) | 72.50 | p-ERK(T202/Y204) | 614.52 | 2 | WM115-signal1 | C10 | |
| Vemurafenib | COLO 858 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 461.92 | p-Rb(S807/S811) | 163.29 | p-ERK(T202/Y204) | 613.35 | 2 | Colo858-signal1 | C9 |