Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 228.13 | p-Rb(S807/S811) | 74.57 | p-ERK(T202/Y204) | 615.48 | 1 | WM115-signal1 | F11 |
| JNK-IN-8 | WM-115 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 227.05 | p-Rb(S807/S811) | 74.53 | p-ERK(T202/Y204) | 625.81 | 1 | WM115-signal1 | D10 | |
| JNK-IN-8 | WM-115 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 238.03 | p-Rb(S807/S811) | 74.48 | p-ERK(T202/Y204) | 630.06 | 1 | WM115-signal1 | D11 | |
| JNK-IN-8 | WM-115 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 223.47 | p-Rb(S807/S811) | 74.36 | p-ERK(T202/Y204) | 609.12 | 1 | WM115-signal1 | D9 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 223.67 | p-Rb(S807/S811) | 74.26 | p-ERK(T202/Y204) | 488.45 | 2 | WM115-signal1 | G8 |
| Vemurafenib | WM1552C | 0.3200 | uM | 24 | hr | | Ki-67 | 1222.80 | p-Rb(S807/S811) | 74.23 | p-cJun(S73) | 158.69 | 2 | WM1552C-signal3 | C5 | |
| Vemurafenib | WM-115 | 0.0000 | uM | 24 | hr | | Ki-67 | 699.08 | p-Rb(S807/S811) | 74.05 | p-cJun(S73) | 48.96 | 2 | WM115-signal3 | C11 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 231.17 | p-Rb(S807/S811) | 73.86 | p-ERK(T202/Y204) | 586.73 | 2 | WM115-signal1 | G11 |
| Vemurafenib | WM-115 | 0.0000 | uM | 24 | hr | | Ki-67 | 738.43 | p-Rb(S807/S811) | 73.77 | p-cJun(S73) | 49.75 | 1 | WM115-signal3 | B11 | |
| JNK-IN-8 | WM-115 | 0.0082 | uM | 24 | hr | | Ki-67 | 787.98 | p-Rb(S807/S811) | 73.62 | p-cJun(S73) | 42.75 | 2 | WM115-signal3 | E9 | |
| Vemurafenib | WM-115 | 0.0082 | uM | 24 | hr | | Ki-67 | 788.63 | p-Rb(S807/S811) | 73.58 | p-cJun(S73) | 49.70 | 1 | WM115-signal3 | B9 | |
| Vemurafenib | WM1552C | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 167.82 | p-Rb(S807/S811) | 73.52 | p-S6(S235/S236 | 355.93 | 2 | WM1552C-signal2 | C5 | |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | Ki-67 | 1979.00 | p-Rb(S807/S811) | 73.51 | p-cJun(S73) | 77.14 | 2 | LOXIMVI-signal3 | C2 | |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 218.07 | p-Rb(S807/S811) | 73.50 | p-S6(S235/S236 | 96.41 | 2 | LOXIMVI-signal2 | C2 | |
| JNK-IN-8 | WM-115 | 0.0082 | uM | 24 | hr | | Ki-67 | 780.27 | p-Rb(S807/S811) | 73.45 | p-cJun(S73) | 43.18 | 1 | WM115-signal3 | D9 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 235.46 | p-Rb(S807/S811) | 73.36 | p-ERK(T202/Y204) | 378.29 | 1 | WM115-signal1 | F7 |
| Vemurafenib | WM-115 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 230.38 | p-Rb(S807/S811) | 73.32 | p-ERK(T202/Y204) | 501.50 | 2 | WM115-signal1 | C8 | |
| Vemurafenib | COLO 858 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 174.16 | p-Rb(S807/S811) | 73.24 | p-S6(S235/S236 | 166.46 | 2 | Colo858-signal2 | C6 | |
| Vemurafenib | WM-115 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 242.95 | p-Rb(S807/S811) | 73.13 | p-ERK(T202/Y204) | 635.44 | 1 | WM115-signal1 | B11 | |
| Vemurafenib | WM-115 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 230.43 | p-Rb(S807/S811) | 73.12 | p-ERK(T202/Y204) | 557.15 | 1 | WM115-signal1 | B9 | |
| Vemurafenib | WM-115 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 238.61 | p-Rb(S807/S811) | 73.09 | p-ERK(T202/Y204) | 620.90 | 2 | WM115-signal1 | C11 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 224.00 | p-Rb(S807/S811) | 72.74 | p-ERK(T202/Y204) | 584.05 | 1 | WM115-signal1 | F10 |
| JNK-IN-8 | WM-115 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 225.01 | p-Rb(S807/S811) | 72.59 | p-ERK(T202/Y204) | 628.25 | 2 | WM115-signal1 | E11 | |
| Vemurafenib | WM-115 | 0.0033 | uM | 24 | hr | | Ki-67 | 734.74 | p-Rb(S807/S811) | 72.53 | p-cJun(S73) | 47.91 | 1 | WM115-signal3 | B10 | |
| Vemurafenib | WM-115 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 224.32 | p-Rb(S807/S811) | 72.50 | p-ERK(T202/Y204) | 614.52 | 2 | WM115-signal1 | C10 |