Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | LOXIMVI | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 282.86 | p-Rb(S807/S811) | 236.08 | p-S6(S235/S236 | 480.10 | 2 | LOXIMVI-signal2 | E6 | |
| Vemurafenib | LOXIMVI | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 304.49 | p-Rb(S807/S811) | 234.76 | p-S6(S235/S236 | 449.34 | 1 | LOXIMVI-signal2 | B11 | |
| JNK-IN-8 | COLO 858 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 355.48 | p-Rb(S807/S811) | 234.13 | p-S6(S235/S236 | 567.56 | 1 | Colo858-signal2 | D3 | |
| JNK-IN-8 | COLO 858 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 596.02 | p-Rb(S807/S811) | 233.59 | p-ERK(T202/Y204) | 707.32 | 1 | Colo858-signal1 | D3 | |
| JNK-IN-8 | LOXIMVI | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 299.94 | p-Rb(S807/S811) | 232.62 | p-S6(S235/S236 | 413.74 | 1 | LOXIMVI-signal2 | D11 | |
| JNK-IN-8 | LOXIMVI | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 285.05 | p-Rb(S807/S811) | 232.52 | p-S6(S235/S236 | 459.23 | 1 | LOXIMVI-signal2 | D7 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 306.56 | p-Rb(S807/S811) | 232.04 | p-S6(S235/S236 | 424.09 | 1 | LOXIMVI-signal2 | F11 |
| JNK-IN-8 | LOXIMVI | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 254.16 | p-Rb(S807/S811) | 231.99 | p-S6(S235/S236 | 458.18 | 2 | LOXIMVI-signal2 | E9 | |
| JNK-IN-8 | COLO 858 | 5.0000 | uM | 24 | hr | | Ki-67 | 2447.10 | p-Rb(S807/S811) | 231.66 | p-cJun(S73) | 37.00 | 1 | Colo858-signal3 | D2 | |
| JNK-IN-8 | LOXIMVI | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 281.63 | p-Rb(S807/S811) | 231.63 | p-S6(S235/S236 | 455.97 | 2 | LOXIMVI-signal2 | E8 | |
| JNK-IN-8 | COLO 858 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 594.04 | p-Rb(S807/S811) | 231.49 | p-ERK(T202/Y204) | 724.41 | 2 | Colo858-signal1 | E3 | |
| JNK-IN-8 | LOXIMVI | 0.8000 | uM | 24 | hr | | p27 Kip1 | 336.96 | p-Rb(S807/S811) | 231.40 | p-ERK(T202/Y204) | 222.11 | 1 | LOXIMVI-signal1 | D4 | |
| JNK-IN-8 | LOXIMVI | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 299.84 | p-Rb(S807/S811) | 230.92 | p-S6(S235/S236 | 450.38 | 1 | LOXIMVI-signal2 | D8 | |
| Vemurafenib | LOXIMVI | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 318.16 | p-Rb(S807/S811) | 230.07 | p-S6(S235/S236 | 423.94 | 2 | LOXIMVI-signal2 | C11 | |
| JNK-IN-8 | LOXIMVI | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 283.60 | p-Rb(S807/S811) | 227.06 | p-S6(S235/S236 | 453.19 | 2 | LOXIMVI-signal2 | E7 | |
| JNK-IN-8 | LOXIMVI | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 242.93 | p-Rb(S807/S811) | 227.00 | p-S6(S235/S236 | 449.29 | 2 | LOXIMVI-signal2 | E10 | |
| JNK-IN-8 | COLO 858 | 2.0000 | uM | 24 | hr | | Ki-67 | 2641.30 | p-Rb(S807/S811) | 226.22 | p-cJun(S73) | 34.79 | 2 | Colo858-signal3 | E3 | |
| JNK-IN-8 | LOXIMVI | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 267.83 | p-Rb(S807/S811) | 225.75 | p-S6(S235/S236 | 426.66 | 1 | LOXIMVI-signal2 | D9 | |
| JNK-IN-8 | LOXIMVI | 0.3200 | uM | 24 | hr | | p27 Kip1 | 312.28 | p-Rb(S807/S811) | 224.98 | p-ERK(T202/Y204) | 217.75 | 1 | LOXIMVI-signal1 | D5 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 269.36 | p-Rb(S807/S811) | 224.35 | p-S6(S235/S236 | 447.46 | 1 | LOXIMVI-signal2 | F10 |
| JNK-IN-8 | LOXIMVI | 0.0205 | uM | 24 | hr | | Ki-67 | 3052.30 | p-Rb(S807/S811) | 224.01 | p-cJun(S73) | 90.47 | 1 | LOXIMVI-signal3 | D8 | |
| JNK-IN-8 | LOXIMVI | 0.8000 | uM | 24 | hr | | p27 Kip1 | 330.87 | p-Rb(S807/S811) | 223.53 | p-ERK(T202/Y204) | 212.37 | 2 | LOXIMVI-signal1 | E4 | |
| JNK-IN-8 | LOXIMVI | 0.8000 | uM | 24 | hr | | Ki-67 | 3017.80 | p-Rb(S807/S811) | 223.34 | p-cJun(S73) | 56.18 | 2 | LOXIMVI-signal3 | E4 | |
| JNK-IN-8 | LOXIMVI | 0.0000 | uM | 24 | hr | | p27 Kip1 | 351.82 | p-Rb(S807/S811) | 222.97 | p-ERK(T202/Y204) | 225.84 | 2 | LOXIMVI-signal1 | E11 | |
| Vemurafenib | LOXIMVI | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 248.94 | p-Rb(S807/S811) | 222.89 | p-S6(S235/S236 | 425.05 | 2 | LOXIMVI-signal2 | C10 |