Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 458.83 | p-Rb(S807/S811) | 152.02 | p-ERK(T202/Y204) | 459.90 | 2 | Colo858-signal1 | G8 |
| Vemurafenib | COLO 858 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 458.31 | p-Rb(S807/S811) | 151.87 | p-ERK(T202/Y204) | 580.80 | 1 | Colo858-signal1 | B9 | |
| Vemurafenib | COLO 858 | 0.0082 | uM | 24 | hr | | Ki-67 | 2239.40 | p-Rb(S807/S811) | 151.71 | p-cJun(S73) | 29.36 | 1 | Colo858-signal3 | B9 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0082 | uM | 24 | hr | | Ki-67 | 2177.30 | p-Rb(S807/S811) | 151.57 | p-cJun(S73) | 28.39 | 1 | Colo858-signal3 | F9 |
| JNK-IN-8 | COLO 858 | 0.0082 | uM | 24 | hr | | Ki-67 | 2294.40 | p-Rb(S807/S811) | 151.40 | p-cJun(S73) | 29.18 | 2 | Colo858-signal3 | E9 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0000 | uM | 24 | hr | | Ki-67 | 2257.00 | p-Rb(S807/S811) | 151.09 | p-cJun(S73) | 29.94 | 1 | Colo858-signal3 | F11 |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | Ki-67 | 2209.00 | p-Rb(S807/S811) | 151.07 | p-cJun(S73) | 28.62 | 1 | Colo858-signal3 | B11 | |
| JNK-IN-8 | COLO 858 | 0.0082 | uM | 24 | hr | | Ki-67 | 2226.10 | p-Rb(S807/S811) | 150.62 | p-cJun(S73) | 29.95 | 1 | Colo858-signal3 | D9 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | Ki-67 | 2146.20 | p-Rb(S807/S811) | 150.60 | p-cJun(S73) | 28.79 | 1 | Colo858-signal3 | F8 |
| JNK-IN-8 | COLO 858 | 0.0033 | uM | 24 | hr | | Ki-67 | 2257.20 | p-Rb(S807/S811) | 149.91 | p-cJun(S73) | 29.66 | 1 | Colo858-signal3 | D10 | |
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 496.15 | p-Rb(S807/S811) | 149.24 | p-ERK(T202/Y204) | 495.70 | 1 | Colo858-signal1 | B8 | |
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | Ki-67 | 2103.40 | p-Rb(S807/S811) | 149.11 | p-cJun(S73) | 30.17 | 1 | Colo858-signal3 | B8 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.1280 | uM | 24 | hr | | Ki-67 | 2465.90 | p-Rb(S807/S811) | 148.93 | p-cJun(S73) | 37.40 | 2 | LOXIMVI-signal3 | G6 |
| Vemurafenib | LOXIMVI | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 249.36 | p-Rb(S807/S811) | 148.83 | p-S6(S235/S236 | 383.67 | 2 | LOXIMVI-signal2 | C5 | |
| JNK-IN-8 | COLO 858 | 0.0000 | uM | 24 | hr | | Ki-67 | 2186.70 | p-Rb(S807/S811) | 147.06 | p-cJun(S73) | 28.84 | 1 | Colo858-signal3 | D11 | |
| Vemurafenib | COLO 858 | 0.0082 | uM | 24 | hr | | Ki-67 | 2183.20 | p-Rb(S807/S811) | 146.71 | p-cJun(S73) | 28.18 | 2 | Colo858-signal3 | C9 | |
| Vemurafenib | COLO 858 | 0.0033 | uM | 24 | hr | | Ki-67 | 2154.80 | p-Rb(S807/S811) | 145.55 | p-cJun(S73) | 28.64 | 1 | Colo858-signal3 | B10 | |
| Vemurafenib | COLO 858 | 0.0033 | uM | 24 | hr | | Ki-67 | 2240.50 | p-Rb(S807/S811) | 144.95 | p-cJun(S73) | 28.17 | 2 | Colo858-signal3 | C10 | |
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | Ki-67 | 2138.80 | p-Rb(S807/S811) | 144.45 | p-cJun(S73) | 30.43 | 2 | Colo858-signal3 | C8 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 219.70 | p-Rb(S807/S811) | 143.66 | p-S6(S235/S236 | 371.59 | 1 | Colo858-signal2 | F7 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 239.08 | p-Rb(S807/S811) | 140.42 | p-S6(S235/S236 | 380.85 | 2 | Colo858-signal2 | G7 |
| Vemurafenib | COLO 858 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 274.60 | p-Rb(S807/S811) | 140.30 | p-S6(S235/S236 | 340.20 | 2 | Colo858-signal2 | C7 | |
| Vemurafenib | COLO 858 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 284.31 | p-Rb(S807/S811) | 139.79 | p-S6(S235/S236 | 349.95 | 1 | Colo858-signal2 | B7 | |
| Vemurafenib | LOXIMVI | 0.8000 | uM | 24 | hr | | p27 Kip1 | 437.65 | p-Rb(S807/S811) | 139.21 | p-ERK(T202/Y204) | 46.29 | 1 | LOXIMVI-signal1 | B4 | |
| Vemurafenib | LOXIMVI | 0.3200 | uM | 24 | hr | | Ki-67 | 2470.00 | p-Rb(S807/S811) | 138.22 | p-cJun(S73) | 75.91 | 1 | LOXIMVI-signal3 | B5 |