Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 452.92 | p-Rb(S807/S811) | 154.74 | p-ERK(T202/Y204) | 648.89 | 2 | Colo858-signal1 | C11 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0000 | uM | 24 | hr | | Ki-67 | 1640.70 | p-Rb(S807/S811) | 107.50 | p-cJun(S73) | 145.58 | 1 | WM1552C-signal3 | F11 |
| JNK-IN-8 | COLO 858 | 0.0000 | uM | 24 | hr | | Ki-67 | 2266.10 | p-Rb(S807/S811) | 152.55 | p-cJun(S73) | 28.74 | 2 | Colo858-signal3 | E11 | |
| JNK-IN-8 | COLO 858 | 0.0000 | uM | 24 | hr | | Ki-67 | 2186.70 | p-Rb(S807/S811) | 147.06 | p-cJun(S73) | 28.84 | 1 | Colo858-signal3 | D11 | |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | Ki-67 | 2259.80 | p-Rb(S807/S811) | 154.64 | p-cJun(S73) | 29.83 | 2 | Colo858-signal3 | C11 | |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | Ki-67 | 2209.00 | p-Rb(S807/S811) | 151.07 | p-cJun(S73) | 28.62 | 1 | Colo858-signal3 | B11 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 376.71 | p-Rb(S807/S811) | 165.48 | p-S6(S235/S236 | 397.13 | 2 | Colo858-signal2 | G11 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 336.86 | p-Rb(S807/S811) | 161.69 | p-S6(S235/S236 | 384.38 | 1 | Colo858-signal2 | F11 |
| JNK-IN-8 | COLO 858 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 357.02 | p-Rb(S807/S811) | 174.95 | p-S6(S235/S236 | 420.53 | 2 | Colo858-signal2 | E11 | |
| JNK-IN-8 | COLO 858 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 323.83 | p-Rb(S807/S811) | 165.88 | p-S6(S235/S236 | 385.36 | 1 | Colo858-signal2 | D11 | |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 479.59 | p-Rb(S807/S811) | 159.95 | p-ERK(T202/Y204) | 654.45 | 1 | Colo858-signal1 | B11 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0000 | uM | 24 | hr | | Ki-67 | 1730.30 | p-Rb(S807/S811) | 108.00 | p-cJun(S73) | 145.49 | 2 | WM1552C-signal3 | G11 |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 377.25 | p-Rb(S807/S811) | 161.02 | p-S6(S235/S236 | 380.19 | 2 | Colo858-signal2 | C11 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 208.77 | p-Rb(S807/S811) | 116.05 | p-S6(S235/S236 | 419.33 | 1 | WM1552C-signal2 | F11 |
| JNK-IN-8 | WM1552C | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 200.92 | p-Rb(S807/S811) | 113.95 | p-S6(S235/S236 | 415.95 | 2 | WM1552C-signal2 | E11 | |
| JNK-IN-8 | WM1552C | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 234.24 | p-Rb(S807/S811) | 115.31 | p-S6(S235/S236 | 377.79 | 1 | WM1552C-signal2 | D11 | |
| Vemurafenib | WM1552C | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 216.59 | p-Rb(S807/S811) | 117.74 | p-S6(S235/S236 | 410.72 | 2 | WM1552C-signal2 | C11 | |
| Vemurafenib | WM1552C | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 221.69 | p-Rb(S807/S811) | 117.69 | p-S6(S235/S236 | 423.70 | 1 | WM1552C-signal2 | B11 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 251.50 | p-Rb(S807/S811) | 108.70 | p-ERK(T202/Y204) | 256.82 | 2 | WM1552C-signal1 | G11 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 239.37 | p-Rb(S807/S811) | 107.35 | p-ERK(T202/Y204) | 253.29 | 1 | WM1552C-signal1 | F11 |