Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | WM-115 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 242.95 | p-Rb(S807/S811) | 73.13 | p-ERK(T202/Y204) | 635.44 | 1 | WM115-signal1 | B11 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0000 | uM | 24 | hr | | Ki-67 | 2934.10 | p-Rb(S807/S811) | 203.26 | p-cJun(S73) | 120.51 | 2 | LOXIMVI-signal3 | G11 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0000 | uM | 24 | hr | | Ki-67 | 3032.30 | p-Rb(S807/S811) | 209.50 | p-cJun(S73) | 127.94 | 1 | LOXIMVI-signal3 | F11 |
| JNK-IN-8 | LOXIMVI | 0.0000 | uM | 24 | hr | | Ki-67 | 3030.30 | p-Rb(S807/S811) | 205.20 | p-cJun(S73) | 122.59 | 2 | LOXIMVI-signal3 | E11 | |
| JNK-IN-8 | LOXIMVI | 0.0000 | uM | 24 | hr | | Ki-67 | 3098.40 | p-Rb(S807/S811) | 213.41 | p-cJun(S73) | 128.11 | 1 | LOXIMVI-signal3 | D11 | |
| JNK-IN-8 | COLO 858 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 445.15 | p-Rb(S807/S811) | 158.36 | p-ERK(T202/Y204) | 601.12 | 2 | Colo858-signal1 | E11 | |
| JNK-IN-8 | WM1552C | 0.0000 | uM | 24 | hr | | Ki-67 | 1603.40 | p-Rb(S807/S811) | 107.85 | p-cJun(S73) | 142.51 | 1 | WM1552C-signal3 | D11 | |
| Vemurafenib | LOXIMVI | 0.0000 | uM | 24 | hr | | Ki-67 | 3001.50 | p-Rb(S807/S811) | 195.98 | p-cJun(S73) | 118.37 | 2 | LOXIMVI-signal3 | C11 | |
| Vemurafenib | LOXIMVI | 0.0000 | uM | 24 | hr | | Ki-67 | 3070.70 | p-Rb(S807/S811) | 206.62 | p-cJun(S73) | 122.85 | 1 | LOXIMVI-signal3 | B11 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 325.81 | p-Rb(S807/S811) | 222.34 | p-S6(S235/S236 | 438.90 | 2 | LOXIMVI-signal2 | G11 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 306.56 | p-Rb(S807/S811) | 232.04 | p-S6(S235/S236 | 424.09 | 1 | LOXIMVI-signal2 | F11 |
| JNK-IN-8 | LOXIMVI | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 312.66 | p-Rb(S807/S811) | 236.29 | p-S6(S235/S236 | 442.43 | 2 | LOXIMVI-signal2 | E11 | |
| JNK-IN-8 | LOXIMVI | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 299.94 | p-Rb(S807/S811) | 232.62 | p-S6(S235/S236 | 413.74 | 1 | LOXIMVI-signal2 | D11 | |
| Vemurafenib | LOXIMVI | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 318.16 | p-Rb(S807/S811) | 230.07 | p-S6(S235/S236 | 423.94 | 2 | LOXIMVI-signal2 | C11 | |
| Vemurafenib | LOXIMVI | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 304.49 | p-Rb(S807/S811) | 234.76 | p-S6(S235/S236 | 449.34 | 1 | LOXIMVI-signal2 | B11 | |
| JNK-IN-8 | COLO 858 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 454.18 | p-Rb(S807/S811) | 159.65 | p-ERK(T202/Y204) | 628.56 | 1 | Colo858-signal1 | D11 | |
| JNK-IN-8 | WM1552C | 0.0000 | uM | 24 | hr | | Ki-67 | 1658.40 | p-Rb(S807/S811) | 108.24 | p-cJun(S73) | 142.64 | 2 | WM1552C-signal3 | E11 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 351.99 | p-Rb(S807/S811) | 215.31 | p-ERK(T202/Y204) | 217.61 | 2 | LOXIMVI-signal1 | G11 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 362.35 | p-Rb(S807/S811) | 212.69 | p-ERK(T202/Y204) | 226.97 | 1 | LOXIMVI-signal1 | F11 |
| JNK-IN-8 | LOXIMVI | 0.0000 | uM | 24 | hr | | p27 Kip1 | 351.82 | p-Rb(S807/S811) | 222.97 | p-ERK(T202/Y204) | 225.84 | 2 | LOXIMVI-signal1 | E11 | |
| JNK-IN-8 | LOXIMVI | 0.0000 | uM | 24 | hr | | p27 Kip1 | 348.21 | p-Rb(S807/S811) | 217.92 | p-ERK(T202/Y204) | 232.50 | 1 | LOXIMVI-signal1 | D11 | |
| Vemurafenib | LOXIMVI | 0.0000 | uM | 24 | hr | | p27 Kip1 | 330.81 | p-Rb(S807/S811) | 208.35 | p-ERK(T202/Y204) | 212.82 | 2 | LOXIMVI-signal1 | C11 | |
| Vemurafenib | LOXIMVI | 0.0000 | uM | 24 | hr | | p27 Kip1 | 346.49 | p-Rb(S807/S811) | 215.22 | p-ERK(T202/Y204) | 215.11 | 1 | LOXIMVI-signal1 | B11 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0000 | uM | 24 | hr | | Ki-67 | 2392.50 | p-Rb(S807/S811) | 158.97 | p-cJun(S73) | 29.83 | 2 | Colo858-signal3 | G11 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0000 | uM | 24 | hr | | Ki-67 | 2257.00 | p-Rb(S807/S811) | 151.09 | p-cJun(S73) | 29.94 | 1 | Colo858-signal3 | F11 |