Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | WM-115 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 231.96 | p-Rb(S807/S811) | 70.30 | p-ERK(T202/Y204) | 503.00 | 1 | WM115-signal1 | B8 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 245.42 | p-Rb(S807/S811) | 98.27 | p-ERK(T202/Y204) | 209.11 | 2 | WM1552C-signal1 | G8 |
| Vemurafenib | WM-115 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 196.19 | p-Rb(S807/S811) | 67.83 | p-S6(S235/S236 | 199.48 | 1 | WM115-signal2 | B8 | |
| JNK-IN-8 | WM1552C | 0.0205 | uM | 24 | hr | | Ki-67 | 1616.70 | p-Rb(S807/S811) | 107.97 | p-cJun(S73) | 148.06 | 1 | WM1552C-signal3 | D8 | |
| Vemurafenib | WM1552C | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 217.23 | p-Rb(S807/S811) | 107.32 | p-S6(S235/S236 | 400.59 | 1 | WM1552C-signal2 | B9 | |
| JNK-IN-8 | WM1552C | 0.0082 | uM | 24 | hr | | Ki-67 | 1563.50 | p-Rb(S807/S811) | 102.65 | p-cJun(S73) | 151.31 | 1 | WM1552C-signal3 | D9 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0082 | uM | 24 | hr | | Ki-67 | 1581.10 | p-Rb(S807/S811) | 104.30 | p-cJun(S73) | 145.59 | 2 | WM1552C-signal3 | G9 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 307.93 | p-Rb(S807/S811) | 203.95 | p-ERK(T202/Y204) | 169.30 | 2 | LOXIMVI-signal1 | G9 |
| JNK-IN-8 | LOXIMVI | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 267.83 | p-Rb(S807/S811) | 225.75 | p-S6(S235/S236 | 426.66 | 1 | LOXIMVI-signal2 | D9 | |
| Vemurafenib | WM1552C | 0.0082 | uM | 24 | hr | | Ki-67 | 1538.30 | p-Rb(S807/S811) | 102.40 | p-cJun(S73) | 142.93 | 2 | WM1552C-signal3 | C9 | |
| JNK-IN-8 | LOXIMVI | 0.0082 | uM | 24 | hr | | p27 Kip1 | 300.54 | p-Rb(S807/S811) | 201.98 | p-ERK(T202/Y204) | 205.87 | 2 | LOXIMVI-signal1 | E9 | |
| Vemurafenib | WM-115 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 248.41 | p-Rb(S807/S811) | 70.27 | p-S6(S235/S236 | 185.43 | 2 | WM115-signal2 | C9 | |
| JNK-IN-8 | COLO 858 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 373.77 | p-Rb(S807/S811) | 163.58 | p-S6(S235/S236 | 386.62 | 2 | Colo858-signal2 | E9 | |
| Vemurafenib | WM1552C | 0.0082 | uM | 24 | hr | | p27 Kip1 | 242.69 | p-Rb(S807/S811) | 101.68 | p-ERK(T202/Y204) | 236.72 | 1 | WM1552C-signal1 | B9 | |
| JNK-IN-8 | WM-115 | 0.0082 | uM | 24 | hr | | Ki-67 | 780.27 | p-Rb(S807/S811) | 73.45 | p-cJun(S73) | 43.18 | 1 | WM115-signal3 | D9 | |
| JNK-IN-8 | WM1552C | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 184.24 | p-Rb(S807/S811) | 113.51 | p-S6(S235/S236 | 409.57 | 1 | WM1552C-signal2 | D9 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0082 | uM | 24 | hr | | Ki-67 | 2667.60 | p-Rb(S807/S811) | 189.57 | p-cJun(S73) | 84.40 | 2 | LOXIMVI-signal3 | G9 |
| Vemurafenib | WM1552C | 0.0082 | uM | 24 | hr | | p27 Kip1 | 241.99 | p-Rb(S807/S811) | 106.90 | p-ERK(T202/Y204) | 245.11 | 2 | WM1552C-signal1 | C9 | |
| JNK-IN-8 | WM-115 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 226.04 | p-Rb(S807/S811) | 77.90 | p-ERK(T202/Y204) | 670.95 | 2 | WM115-signal1 | E9 | |
| JNK-IN-8 | COLO 858 | 0.0082 | uM | 24 | hr | | Ki-67 | 2226.10 | p-Rb(S807/S811) | 150.62 | p-cJun(S73) | 29.95 | 1 | Colo858-signal3 | D9 | |
| Vemurafenib | WM1552C | 0.0082 | uM | 24 | hr | | Ki-67 | 1588.00 | p-Rb(S807/S811) | 105.77 | p-cJun(S73) | 146.98 | 1 | WM1552C-signal3 | B9 | |
| JNK-IN-8 | WM-115 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 222.98 | p-Rb(S807/S811) | 70.29 | p-S6(S235/S236 | 190.36 | 1 | WM115-signal2 | D9 | |
| Vemurafenib | LOXIMVI | 0.0082 | uM | 24 | hr | | Ki-67 | 2983.70 | p-Rb(S807/S811) | 203.05 | p-cJun(S73) | 109.97 | 2 | LOXIMVI-signal3 | C9 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0082 | uM | 24 | hr | | Ki-67 | 732.41 | p-Rb(S807/S811) | 74.91 | p-cJun(S73) | 46.30 | 2 | WM115-signal3 | G9 |
| Vemurafenib | COLO 858 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 458.31 | p-Rb(S807/S811) | 151.87 | p-ERK(T202/Y204) | 580.80 | 1 | Colo858-signal1 | B9 |