Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | WM1552C | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 167.82 | p-Rb(S807/S811) | 73.52 | p-S6(S235/S236 | 355.93 | 2 | WM1552C-signal2 | C5 | |
| Vemurafenib | WM1552C | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 144.13 | p-Rb(S807/S811) | 66.51 | p-S6(S235/S236 | 373.65 | 1 | WM1552C-signal2 | B5 | |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 1028.10 | p-Rb(S807/S811) | 48.93 | p-ERK(T202/Y204) | 27.49 | 2 | Colo858-signal1 | C5 | |
| JNK-IN-8 | LOXIMVI | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 260.69 | p-Rb(S807/S811) | 241.93 | p-S6(S235/S236 | 501.67 | 2 | LOXIMVI-signal2 | E5 | |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | Ki-67 | 660.32 | p-Rb(S807/S811) | 48.67 | p-cJun(S73) | 67.95 | 2 | Colo858-signal3 | C5 | |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 144.45 | p-Rb(S807/S811) | 54.34 | p-S6(S235/S236 | 77.03 | 1 | Colo858-signal2 | B5 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 277.07 | p-Rb(S807/S811) | 41.08 | p-ERK(T202/Y204) | 52.64 | 1 | WM115-signal1 | F5 |
| JNK-IN-8 | LOXIMVI | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 291.53 | p-Rb(S807/S811) | 246.99 | p-S6(S235/S236 | 502.86 | 1 | LOXIMVI-signal2 | D5 | |
| Vemurafenib | LOXIMVI | 0.3200 | uM | 24 | hr | | Ki-67 | 2470.00 | p-Rb(S807/S811) | 138.22 | p-cJun(S73) | 75.91 | 1 | LOXIMVI-signal3 | B5 | |
| Vemurafenib | WM-115 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 257.65 | p-Rb(S807/S811) | 38.40 | p-ERK(T202/Y204) | 44.96 | 2 | WM115-signal1 | C5 | |
| JNK-IN-8 | WM-115 | 0.3200 | uM | 24 | hr | | Ki-67 | 814.81 | p-Rb(S807/S811) | 81.21 | p-cJun(S73) | 22.95 | 1 | WM115-signal3 | D5 | |
| JNK-IN-8 | WM-115 | 0.3200 | uM | 24 | hr | | Ki-67 | 785.96 | p-Rb(S807/S811) | 71.52 | p-cJun(S73) | 21.74 | 2 | WM115-signal3 | E5 | |
| Vemurafenib | LOXIMVI | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 249.36 | p-Rb(S807/S811) | 148.83 | p-S6(S235/S236 | 383.67 | 2 | LOXIMVI-signal2 | C5 | |
| Vemurafenib | COLO 858 | 0.1280 | uM | 24 | hr | | Ki-67 | 972.60 | p-Rb(S807/S811) | 76.98 | p-cJun(S73) | 64.88 | 1 | Colo858-signal3 | B6 | |
| Vemurafenib | WM-115 | 0.1280 | uM | 24 | hr | | Ki-67 | 784.48 | p-Rb(S807/S811) | 55.88 | p-cJun(S73) | 67.92 | 1 | WM115-signal3 | B6 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 279.48 | p-Rb(S807/S811) | 87.64 | p-ERK(T202/Y204) | 117.21 | 1 | WM1552C-signal1 | F6 |
| Vemurafenib | LOXIMVI | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 282.64 | p-Rb(S807/S811) | 182.20 | p-S6(S235/S236 | 400.81 | 1 | LOXIMVI-signal2 | B6 | |
| Vemurafenib | WM-115 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 237.94 | p-Rb(S807/S811) | 55.20 | p-ERK(T202/Y204) | 149.42 | 1 | WM115-signal1 | B6 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 245.83 | p-Rb(S807/S811) | 61.24 | p-ERK(T202/Y204) | 166.19 | 2 | WM115-signal1 | G6 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 348.86 | p-Rb(S807/S811) | 169.33 | p-ERK(T202/Y204) | 67.27 | 2 | LOXIMVI-signal1 | G6 |
| JNK-IN-8 | WM1552C | 0.1280 | uM | 24 | hr | | p27 Kip1 | 230.98 | p-Rb(S807/S811) | 107.78 | p-ERK(T202/Y204) | 265.42 | 2 | WM1552C-signal1 | E6 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 194.38 | p-Rb(S807/S811) | 84.75 | p-S6(S235/S236 | 196.38 | 2 | Colo858-signal2 | G6 |
| JNK-IN-8 | LOXIMVI | 0.1280 | uM | 24 | hr | | Ki-67 | 2922.80 | p-Rb(S807/S811) | 202.58 | p-cJun(S73) | 68.33 | 1 | LOXIMVI-signal3 | D6 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 356.71 | p-Rb(S807/S811) | 166.33 | p-ERK(T202/Y204) | 71.63 | 1 | LOXIMVI-signal1 | F6 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 743.36 | p-Rb(S807/S811) | 68.92 | p-ERK(T202/Y204) | 51.05 | 2 | Colo858-signal1 | G6 |