Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 1028.10 | p-Rb(S807/S811) | 48.93 | p-ERK(T202/Y204) | 27.49 | 2 | Colo858-signal1 | C5 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | Ki-67 | 756.62 | p-Rb(S807/S811) | 56.61 | p-cJun(S73) | 22.24 | 2 | Colo858-signal3 | G5 |
| Vemurafenib | WM1552C | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 144.13 | p-Rb(S807/S811) | 66.51 | p-S6(S235/S236 | 373.65 | 1 | WM1552C-signal2 | B5 | |
| JNK-IN-8 | COLO 858 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 367.65 | p-Rb(S807/S811) | 192.54 | p-S6(S235/S236 | 440.93 | 1 | Colo858-signal2 | D5 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 336.58 | p-Rb(S807/S811) | 69.57 | p-ERK(T202/Y204) | 57.54 | 2 | WM1552C-signal1 | G5 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | Ki-67 | 797.23 | p-Rb(S807/S811) | 51.78 | p-cJun(S73) | 20.45 | 1 | Colo858-signal3 | F5 |
| JNK-IN-8 | WM-115 | 0.3200 | uM | 24 | hr | | Ki-67 | 814.81 | p-Rb(S807/S811) | 81.21 | p-cJun(S73) | 22.95 | 1 | WM115-signal3 | D5 | |
| Vemurafenib | LOXIMVI | 0.3200 | uM | 24 | hr | | Ki-67 | 2387.10 | p-Rb(S807/S811) | 134.09 | p-cJun(S73) | 83.10 | 2 | LOXIMVI-signal3 | C5 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 343.75 | p-Rb(S807/S811) | 67.08 | p-ERK(T202/Y204) | 51.17 | 1 | WM1552C-signal1 | F5 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 237.24 | p-Rb(S807/S811) | 135.99 | p-S6(S235/S236 | 359.46 | 1 | LOXIMVI-signal2 | F5 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.3200 | uM | 24 | hr | | Ki-67 | 2301.20 | p-Rb(S807/S811) | 115.21 | p-cJun(S73) | 27.54 | 2 | LOXIMVI-signal3 | G5 |
| Vemurafenib | WM-115 | 0.3200 | uM | 24 | hr | | Ki-67 | 587.55 | p-Rb(S807/S811) | 26.27 | p-cJun(S73) | 71.93 | 2 | WM115-signal3 | C5 | |
| Vemurafenib | WM1552C | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 167.82 | p-Rb(S807/S811) | 73.52 | p-S6(S235/S236 | 355.93 | 2 | WM1552C-signal2 | C5 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 173.97 | p-Rb(S807/S811) | 36.66 | p-S6(S235/S236 | 72.75 | 2 | WM115-signal2 | G5 |
| JNK-IN-8 | COLO 858 | 0.3200 | uM | 24 | hr | | Ki-67 | 2300.70 | p-Rb(S807/S811) | 172.32 | p-cJun(S73) | 30.64 | 2 | Colo858-signal3 | E5 | |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 150.91 | p-Rb(S807/S811) | 54.62 | p-S6(S235/S236 | 93.11 | 2 | Colo858-signal2 | C5 | |
| Vemurafenib | WM-115 | 0.3200 | uM | 24 | hr | | Ki-67 | 721.37 | p-Rb(S807/S811) | 38.52 | p-cJun(S73) | 78.48 | 1 | WM115-signal3 | B5 | |
| JNK-IN-8 | COLO 858 | 0.3200 | uM | 24 | hr | | Ki-67 | 2279.00 | p-Rb(S807/S811) | 170.95 | p-cJun(S73) | 30.51 | 1 | Colo858-signal3 | D5 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 277.07 | p-Rb(S807/S811) | 41.08 | p-ERK(T202/Y204) | 52.64 | 1 | WM115-signal1 | F5 |
| JNK-IN-8 | COLO 858 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 445.16 | p-Rb(S807/S811) | 182.28 | p-ERK(T202/Y204) | 673.10 | 2 | Colo858-signal1 | E5 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.3200 | uM | 24 | hr | | Ki-67 | 2320.10 | p-Rb(S807/S811) | 113.12 | p-cJun(S73) | 29.40 | 1 | LOXIMVI-signal3 | F5 |
| JNK-IN-8 | WM-115 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 232.47 | p-Rb(S807/S811) | 84.23 | p-ERK(T202/Y204) | 633.23 | 2 | WM115-signal1 | E5 | |
| JNK-IN-8 | WM1552C | 0.3200 | uM | 24 | hr | | p27 Kip1 | 238.39 | p-Rb(S807/S811) | 107.84 | p-ERK(T202/Y204) | 264.88 | 2 | WM1552C-signal1 | E5 | |
| JNK-IN-8 | WM1552C | 0.3200 | uM | 24 | hr | | p27 Kip1 | 235.99 | p-Rb(S807/S811) | 105.87 | p-ERK(T202/Y204) | 263.93 | 1 | WM1552C-signal1 | D5 | |
| Vemurafenib | WM1552C | 0.3200 | uM | 24 | hr | | p27 Kip1 | 349.70 | p-Rb(S807/S811) | 71.79 | p-ERK(T202/Y204) | 64.17 | 2 | WM1552C-signal1 | C5 |