Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Gefitinib | HCC1954 | 31.6 | nM | 24 | hr | 271.88 | 133.88 | p-4EBP1 | 138.34 | |
| Torkinib | HCC1954 | 7.5 | nM | 6 | hr | 264.08 | 62.91 | p-4EBP1 | 67.81 | |
| Torkinib | HCC1954 | 7.5 | nM | 24 | hr | 266.85 | 111.10 | p-4EBP1 | 127.79 | |
| GSK1059615 | HCC1954 | 31.6 | nM | 6 | hr | 429.75 | 93.39 | p-4EBP1 | 123.31 | |
| GSK1059615 | HCC1954 | 31.6 | nM | 24 | hr | 277.08 | 115.06 | p-4EBP1 | 140.49 | |
| MK2206 | HCC1954 | 31.6 | nM | 6 | hr | 287.15 | 82.63 | p-4EBP1 | 114.54 | |
| MK2206 | HCC1954 | 31.6 | nM | 24 | hr | 279.26 | 123.89 | p-4EBP1 | 146.54 | |
| Triciribine | HCC1954 | 31.6 | nM | 6 | hr | 257.88 | 87.64 | p-4EBP1 | 119.50 | |
| Triciribine | HCC1954 | 31.6 | nM | 24 | hr | 283.10 | 118.84 | p-4EBP1 | 123.98 | |
| Dactolisib | HCC1954 | 31.6 | nM | 6 | hr | 184.57 | 70.58 | p-4EBP1 | 27.57 | |
| Dactolisib | HCC1954 | 31.6 | nM | 24 | hr | 258.30 | 89.82 | p-4EBP1 | 37.14 | |
| Gefitinib | MCF 10A | 31.6 | nM | 6 | hr | 397.92 | 167.11 | p-4EBP1 | 286.87 | |
| Gefitinib | MCF 10A | 31.6 | nM | 24 | hr | 296.01 | 168.37 | p-4EBP1 | 199.27 | |
| Torkinib | MCF 10A | 7.5 | nM | 6 | hr | 454.63 | 172.76 | p-4EBP1 | 304.51 | |
| Torkinib | MCF 10A | 7.5 | nM | 24 | hr | 392.70 | 173.87 | p-4EBP1 | 178.68 | |
| GSK1059615 | MCF 10A | 31.6 | nM | 6 | hr | 404.32 | 181.97 | p-4EBP1 | 362.50 | |
| GSK1059615 | MCF 10A | 31.6 | nM | 24 | hr | 325.92 | 153.57 | p-4EBP1 | 239.72 | |
| MK2206 | MCF 10A | 31.6 | nM | 6 | hr | 352.68 | 159.86 | p-4EBP1 | 348.74 | |
| MK2206 | MCF 10A | 31.6 | nM | 24 | hr | 272.79 | 163.64 | p-4EBP1 | 208.49 | |
| Triciribine | MCF 10A | 31.6 | nM | 6 | hr | 355.42 | 160.76 | p-4EBP1 | 350.19 | |
| Triciribine | MCF 10A | 31.6 | nM | 24 | hr | 240.58 | 161.23 | p-4EBP1 | 197.24 | |
| Dactolisib | MCF 10A | 31.6 | nM | 6 | hr | 371.08 | 143.77 | p-4EBP1 | 198.52 | |
| Dactolisib | MCF 10A | 31.6 | nM | 24 | hr | 259.65 | 147.71 | p-4EBP1 | 83.58 | |
| Gefitinib | T47D | 31.6 | nM | 6 | hr | 171.24 | 91.49 | p-4EBP1 | 465.53 | |
| Gefitinib | T47D | 31.6 | nM | 24 | hr | 153.72 | 115.23 | p-4EBP1 | 404.00 | |