Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Dactolisib | AU565 | 10.0 | nM | 24 | hr | 458.05 | 99.05 | p-4EBP1 | 135.30 | |
| Gefitinib | HCC1954 | 10.0 | nM | 6 | hr | 275.41 | 73.18 | p-4EBP1 | 80.92 | |
| Gefitinib | HCC1954 | 10.0 | nM | 24 | hr | 288.71 | 138.39 | p-4EBP1 | 147.90 | |
| Torkinib | HCC1954 | 1.78 | nM | 6 | hr | 293.07 | 65.74 | p-4EBP1 | 74.89 | |
| Torkinib | HCC1954 | 1.78 | nM | 24 | hr | 279.49 | 117.73 | p-4EBP1 | 127.32 | |
| GSK1059615 | HCC1954 | 10.0 | nM | 6 | hr | 371.06 | 87.71 | p-4EBP1 | 105.89 | |
| GSK1059615 | HCC1954 | 10.0 | nM | 24 | hr | 299.57 | 120.02 | p-4EBP1 | 137.52 | |
| MK2206 | HCC1954 | 10.0 | nM | 6 | hr | 296.60 | 84.33 | p-4EBP1 | 119.17 | |
| MK2206 | HCC1954 | 10.0 | nM | 24 | hr | 268.66 | 124.88 | p-4EBP1 | 149.28 | |
| Triciribine | HCC1954 | 10.0 | nM | 6 | hr | 179.66 | 55.22 | p-4EBP1 | 83.92 | |
| Triciribine | HCC1954 | 10.0 | nM | 24 | hr | 296.63 | 121.16 | p-4EBP1 | 129.91 | |
| Dactolisib | HCC1954 | 10.0 | nM | 6 | hr | 190.63 | 71.18 | p-4EBP1 | 60.40 | |
| Dactolisib | HCC1954 | 10.0 | nM | 24 | hr | 283.72 | 103.87 | p-4EBP1 | 92.54 | |
| Gefitinib | MCF 10A | 10.0 | nM | 6 | hr | 410.41 | 173.88 | p-4EBP1 | 293.08 | |
| Gefitinib | MCF 10A | 10.0 | nM | 24 | hr | 294.37 | 172.89 | p-4EBP1 | 220.40 | |
| Torkinib | MCF 10A | 1.78 | nM | 6 | hr | 452.12 | 168.36 | p-4EBP1 | 335.74 | |
| Torkinib | MCF 10A | 1.78 | nM | 24 | hr | 378.01 | 169.49 | p-4EBP1 | 228.91 | |
| GSK1059615 | MCF 10A | 10.0 | nM | 6 | hr | 397.02 | 180.42 | p-4EBP1 | 352.80 | |
| GSK1059615 | MCF 10A | 10.0 | nM | 24 | hr | 310.71 | 152.34 | p-4EBP1 | 239.86 | |
| MK2206 | MCF 10A | 10.0 | nM | 6 | hr | 379.18 | 154.93 | p-4EBP1 | 370.05 | |
| MK2206 | MCF 10A | 10.0 | nM | 24 | hr | 302.75 | 167.85 | p-4EBP1 | 219.96 | |
| Triciribine | MCF 10A | 10.0 | nM | 6 | hr | 383.05 | 155.63 | p-4EBP1 | 371.60 | |
| Triciribine | MCF 10A | 10.0 | nM | 24 | hr | 239.76 | 149.40 | p-4EBP1 | 223.58 | |
| Dactolisib | MCF 10A | 10.0 | nM | 6 | hr | 332.66 | 147.91 | p-4EBP1 | 311.40 | |
| Dactolisib | MCF 10A | 10.0 | nM | 24 | hr | 230.99 | 147.76 | p-4EBP1 | 178.76 | |