Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Triciribine | MCF 10A | 1.0 | nM | 6 | hr | 468.07 | 156.41 | p-4EBP1 | 394.50 | |
| Triciribine | MCF 10A | 1.0 | nM | 24 | hr | 271.13 | 139.86 | p-4EBP1 | 293.74 | |
| Dactolisib | MCF 10A | 1.0 | nM | 6 | hr | 422.75 | 163.30 | p-4EBP1 | 379.78 | |
| Dactolisib | MCF 10A | 1.0 | nM | 24 | hr | 262.81 | 138.59 | p-4EBP1 | 280.35 | |
| Gefitinib | T47D | 1.0 | nM | 6 | hr | 190.48 | 96.43 | p-4EBP1 | 457.97 | |
| Gefitinib | T47D | 1.0 | nM | 24 | hr | 165.45 | 112.53 | p-4EBP1 | 413.72 | |
| Torkinib | T47D | 0.1 | nM | 6 | hr | 175.05 | 87.63 | p-4EBP1 | 497.19 | |
| Torkinib | T47D | 0.1 | nM | 24 | hr | 148.84 | 105.12 | p-4EBP1 | 393.53 | |
| GSK1059615 | T47D | 1.0 | nM | 6 | hr | 166.53 | 87.01 | p-4EBP1 | 465.64 | |
| GSK1059615 | T47D | 1.0 | nM | 24 | hr | 157.62 | 101.03 | p-4EBP1 | 404.11 | |
| MK2206 | T47D | 1.0 | nM | 6 | hr | 88.69 | 32.40 | p-4EBP1 | 70.25 | |
| MK2206 | T47D | 1.0 | nM | 24 | hr | 144.87 | 107.62 | p-4EBP1 | 379.86 | |
| Triciribine | T47D | 1.0 | nM | 6 | hr | 99.51 | 46.00 | p-4EBP1 | 135.71 | |
| Triciribine | T47D | 1.0 | nM | 24 | hr | 149.52 | 106.88 | p-4EBP1 | 369.04 | |
| Dactolisib | T47D | 1.0 | nM | 6 | hr | 92.26 | 27.93 | p-4EBP1 | 75.18 | |
| Dactolisib | T47D | 1.0 | nM | 24 | hr | 143.56 | 106.16 | p-4EBP1 | 333.82 | |
| Gefitinib | AU565 | 3.16 | nM | 6 | hr | 226.81 | 47.26 | p-4EBP1 | 50.71 | |
| Gefitinib | AU565 | 3.16 | nM | 24 | hr | 578.56 | 134.27 | p-4EBP1 | 156.00 | |
| Torkinib | AU565 | 0.422 | nM | 6 | hr | 372.97 | 73.36 | p-4EBP1 | 96.01 | |
| Torkinib | AU565 | 0.422 | nM | 24 | hr | 462.74 | 121.07 | p-4EBP1 | 200.89 | |
| GSK1059615 | AU565 | 3.16 | nM | 6 | hr | 315.62 | 78.83 | p-4EBP1 | 96.10 | |
| GSK1059615 | AU565 | 3.16 | nM | 24 | hr | 440.71 | 126.76 | p-4EBP1 | 187.62 | |
| MK2206 | AU565 | 3.16 | nM | 6 | hr | 355.42 | 84.04 | p-4EBP1 | 122.33 | |
| MK2206 | AU565 | 3.16 | nM | 24 | hr | 421.91 | 130.26 | p-4EBP1 | 190.28 | |
| Triciribine | AU565 | 3.16 | nM | 6 | hr | 315.25 | 77.31 | p-4EBP1 | 111.67 | |