HMS LINCS Project

Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)

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Small molecules	Cells
10057:MK2206 10098:Gefitinib 10172:GSK1059615 10232:Dactolisib 10280:Triciribine 10345:Torkinib	50091:AU565 50213:HCC1954 50541:T47D 50583:MCF 10A

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Small Molecule	Cell Line	Drug Concentration	Conc Units	Timepoint	Timepoint Units	Measured pERK (Alexa 488)	Measured p-Rb (Alexa 568)	Alexa Fluor 647	Measured p-Akt/p-4EBP1/p-S6 (Alexa 647)	Image
Gefitinib	AU565	100.0	nM	6	hr	215.94	45.64	p-S6	67.64
Gefitinib	AU565	100.0	nM	24	hr	494.19	112.43	p-S6	486.83
Torkinib	AU565	31.6	nM	6	hr	280.20	51.81	p-S6	13.25
Torkinib	AU565	31.6	nM	24	hr	427.57	74.48	p-S6	39.34
GSK1059615	AU565	100.0	nM	6	hr	205.39	49.10	p-S6	81.31
GSK1059615	AU565	100.0	nM	24	hr	479.81	110.27	p-S6	446.19
MK2206	AU565	100.0	nM	6	hr	265.59	64.05	p-S6	81.69
MK2206	AU565	100.0	nM	24	hr	378.47	105.02	p-S6	348.62
Triciribine	AU565	100.0	nM	6	hr	298.70	77.47	p-S6	170.69
Triciribine	AU565	100.0	nM	24	hr	594.21	121.58	p-S6	442.44
Dactolisib	AU565	100.0	nM	6	hr	225.60	44.84	p-S6	12.00
Dactolisib	AU565	100.0	nM	24	hr	448.03	61.93	p-S6	14.23
Gefitinib	HCC1954	100.0	nM	6	hr	200.47	72.75	p-S6	137.48
Gefitinib	HCC1954	100.0	nM	24	hr	269.73	119.60	p-S6	313.66
Torkinib	HCC1954	31.6	nM	6	hr	150.95	28.84	p-S6	27.08
Torkinib	HCC1954	31.6	nM	24	hr	248.45	102.30	p-S6	448.86
GSK1059615	HCC1954	100.0	nM	6	hr	419.11	103.44	p-S6	388.82
GSK1059615	HCC1954	100.0	nM	24	hr	306.81	108.19	p-S6	369.67
MK2206	HCC1954	100.0	nM	6	hr	183.77	54.57	p-S6	114.13
MK2206	HCC1954	100.0	nM	24	hr	251.11	108.59	p-S6	282.07
Triciribine	HCC1954	100.0	nM	6	hr	247.62	90.12	p-S6	371.41
Triciribine	HCC1954	100.0	nM	24	hr	278.15	105.10	p-S6	296.54
Dactolisib	HCC1954	100.0	nM	6	hr	161.80	35.80	p-S6	8.52
Dactolisib	HCC1954	100.0	nM	24	hr	277.05	85.11	p-S6	17.62
Gefitinib	MCF 10A	100.0	nM	6	hr	352.15	168.35	p-S6	446.69

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