Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| MK2206 | HCC1954 | 10.0 | uM | 6 | hr | 174.46 | 37.76 | p-Akt | 13.98 | |
| MK2206 | HCC1954 | 10.0 | uM | 24 | hr | 255.54 | 59.13 | p-Akt | 18.86 | |
| Triciribine | HCC1954 | 10.0 | uM | 6 | hr | 210.36 | 67.80 | p-Akt | 22.65 | |
| Triciribine | HCC1954 | 10.0 | uM | 24 | hr | 244.11 | 78.00 | p-Akt | 24.77 | |
| Dactolisib | HCC1954 | 10.0 | uM | 6 | hr | 262.10 | 75.51 | p-Akt | 16.71 | |
| Dactolisib | HCC1954 | 10.0 | uM | 24 | hr | 288.74 | 69.44 | p-Akt | 18.00 | |
| Gefitinib | MCF 10A | 10.0 | uM | 6 | hr | 192.75 | 129.16 | p-Akt | 29.39 | |
| Gefitinib | MCF 10A | 10.0 | uM | 24 | hr | 144.97 | 21.29 | p-Akt | 18.43 | |
| Torkinib | MCF 10A | 10.0 | uM | 6 | hr | 534.74 | 132.52 | p-Akt | 15.37 | |
| Torkinib | MCF 10A | 10.0 | uM | 24 | hr | 385.16 | 67.00 | p-Akt | 11.02 | |
| GSK1059615 | MCF 10A | 10.0 | uM | 6 | hr | 194.32 | 116.59 | p-Akt | 14.24 | |
| GSK1059615 | MCF 10A | 10.0 | uM | 24 | hr | 197.93 | 71.11 | p-Akt | 12.70 | |
| MK2206 | MCF 10A | 10.0 | uM | 6 | hr | 378.59 | 145.24 | p-Akt | 17.51 | |
| MK2206 | MCF 10A | 10.0 | uM | 24 | hr | 355.11 | 80.95 | p-Akt | 14.03 | |
| Triciribine | MCF 10A | 10.0 | uM | 6 | hr | 379.41 | 146.11 | p-Akt | 17.50 | |
| Triciribine | MCF 10A | 10.0 | uM | 24 | hr | 302.76 | 85.00 | p-Akt | 21.02 | |
| Dactolisib | MCF 10A | 10.0 | uM | 6 | hr | 308.31 | 134.66 | p-Akt | 16.90 | |
| Dactolisib | MCF 10A | 10.0 | uM | 24 | hr | 432.47 | 63.35 | p-Akt | 21.43 | |
| Gefitinib | T47D | 10.0 | uM | 6 | hr | 153.89 | 90.74 | p-Akt | 104.14 | |
| Gefitinib | T47D | 10.0 | uM | 24 | hr | 145.66 | 74.25 | p-Akt | 69.13 | |
| Torkinib | T47D | 10.0 | uM | 6 | hr | 157.33 | 73.56 | p-Akt | 15.39 | |
| Torkinib | T47D | 10.0 | uM | 24 | hr | 125.57 | 36.99 | p-Akt | 13.71 | |
| GSK1059615 | T47D | 10.0 | uM | 6 | hr | 164.72 | 61.51 | p-Akt | 15.01 | |
| GSK1059615 | T47D | 10.0 | uM | 24 | hr | 204.70 | 67.63 | p-Akt | 16.97 | |
| MK2206 | T47D | 10.0 | uM | 6 | hr | 118.02 | 33.64 | p-Akt | 6.86 | |